Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
by capillary electrophoresis in
an ABI Prism 310 Genetic Analyzer
under denaturing conditions.
One microliter of each PCR reaction
or 1 ml of a mixture of PCR
reactions was combined with 9 ml
formamide and 0.51 ml of ROX
1000 size marker. Samples were
then heated to 94 C for 5 minutes and flash-cooled in an ice slurry.
Samples were loaded onto the
machine and electrophoresed at
15 kV, 60 C for 35-45 minutes
using POP4 polymer and filter set
D. The default injection parameters
of 15 kV, 5 seconds were
used. The fragment analysis software
GeneScan (Applied Biosystems,
Inc., Foster City, CA,) was
used to determine peak sizes in
base pairs and peak heights which
were used to calculate NT/CT
ratios. No size correction was
performed since each DNA molecule
was tagged with one fluorescent
marker from one labeled
primer. The area under the curve
values for each NT and CT was
used for calculations.
All statistical analyses were conducted
using SAS version 6.11
(SAS Institute, Cary, NC). A GLM
test followed by Duncan analysis
was used to test for significant differences
in gene expression values
obtained by the three methods of
detection within the same cDNA.
Differences were considered statistically
significant if the P value
was less than 0.05.
Comparison of three methods
Gene expression values for 15
genes were obtained by three
methods of electrophoresis from
the same cDNA, as described. Figure
1 on page 5 depicts representative
results obtained by each of
the three electrophoresis methods.
The agarose gel shows the familiar
band pattern (figure 1A). Although
some of the bands are distorted a
good resolution and quantification
can be obtain
Source:Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
(Date:3/27/2015)... ROCKVILLE, Md. , March 27, 2015 CASI ... dedicated to the acquisition, development and commercialization of innovative ... the global market with a commercial focus on ... the three and 12 months ended December 31, 2014. ... of ($1.6 million), or ($0.05) per share, for the ...
(Date:3/26/2015)... SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: SQD; ... including the appointment of auditors, its annual and special ... "Meeting") was adjourned, as previously disclosed on March 25, ... Toronto time). The date of the ... The Meeting will reconvene at the offices of the ...
(Date:3/26/2015)... March 26, 2015 Neogen Corporation (NASDAQ: ... the third quarter of fiscal 2015, which ended Feb. ... prior year,s $6,575,000. Earnings per share in the current ... Current year-to-date net income increased 17% over prior year ... or $0.56 per share, for the same period a ...
(Date:3/26/2015)... Ale Gicqueau, Founder and President of ... Affairs at Align Technology, on “ Conducting Postmarket Registries ... 2015 Global Conference and Exhibition on April 25th through ... During the poster presentation, Ale Gicqueau and Victor Chen ... Postmarket Registry, the requirements and resources to conduct the ... Breaking Biology Technology:CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 3CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 4CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 5CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 6SQI Diagnostics Inc. Announces Adjournment of Meeting 2Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3
... announced today that the Company will host an investor ... PM Eastern Time to present an,analysis of the data ... IT-R-006) for the treatment of nasolabial folds, or,wrinkles. On ... results from these two studies, which met all primary ...
... BRUNSWICK, N.J., Oct. 20 Senesco,Technologies, Inc. ("Senesco" ... that Catherine Taylor, one of the Company,s funded,researchers, ... multiple myeloma data at the 20th EORTC-NCI-AACR Symposium ... a member of the,Department of Biology at the ...
... -- China Yingxia,International, Inc. (OTC Bulletin Board: ... provider in the health and nutritional food ... of nutritional,food products, dietary supplements, and raw ... ("PRC"), today announced that the Company,s Chief,Financial ...Cached Biology Technology:Isolagen to Host Investor Conference Call to Present an Analysis of the Data from Phase III Wrinkle Studies 2Senesco's Preclinical Multiple Myeloma Data to be Presented at the 20th EORTC-NCI-AACR Symposium on 'Molecular Targets and Cancer Therapeutics' 2CFO of China Yingxia Emphasizes Growth in Organic and Health Food Segment in Interview by The Wall Street Transcript 2CFO of China Yingxia Emphasizes Growth in Organic and Health Food Segment in Interview by The Wall Street Transcript 3
(Date:3/12/2015)... , March 12, 2015 The mascots ... Brenner Children,s Hospital, part of Wake Forest Baptist Medical ... initiative for the 2015 ACC Men,s Basketball Tournament. Young ... with them and getting their autographs. Brenner ... Baptist. It is the only children,s hospital in northwest ...
(Date:3/11/2015)... The Sync Project™ today announced ... and harness music to improve health. The platform ... measurements of physiology, enabling the study of the ... populations. It is designed for medical and health ... studies and accelerate the discovery of the clinical ...
(Date:3/10/2015)... , March 10, 2015 NXT-ID, Inc. (NASDAQ: ... the "Company"), a biometric authentication company focused on ... Wocket™ smart wallet has been named the "Number ... Modern. Rethink Modern ( http://www.rethinkmodern.com/ ) ... for your home and lifestyle that have a unique ... Breaking Biology News(10 mins):Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 2Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 4
... having the right genetic mix as it is to ... at the University of Edinburgh working with researchers at ... happiness is partly determined by personality traits and that ... a framework which psychologists use to rate personalities, called ...
... announced the creation of five new Centers of ... study border security and immigration; explosives detection, mitigation ... disasters, coastal infrastructure and emergency management; and transportation ... Southeastern Tropical Advanced Remote Sensing (CSTARS) will serve ...
... 10 percent each year from 2010 to 2050 to ... but this is an unsustainable prospect. According to a ... Economy and Ecology such a large growth rate will ... each new power plant will simply cannibalize the energy ...Cached Biology News:CSTARS to form part of Department of Homeland Security's Center of Excellence for Maritime Security 2Nuclear cannibals 2