Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
StaRT-PCR was performed using
previously published protocols
with G.E.N.E. system 1a gene
expression kit (Gene Express
National Enterprises, Inc.,
Huntsville, AL). Briefly, a master
mixture containing buffer, MgCl2,
dNTPs, cDNA from normal human
bronchial epithelial cells (lot
17378, American Type Culture Collection,
Rockville, MD), CT mixture
from G.E.N.E. system 1a kit
and Taq polymerase (Promega,
Madison, WI) was prepared.
Aliquots of this mixture were dispensed
into tubes containing genespecific
primers and cycled either
in a Rapidcycler (Idaho Technology,
Inc., Idaho Falls, ID) or PTC-
100 block thermal cycler with
heated lid (MJ Research, Inc.,
Incline Village, NV) for 35 cycles.
In each protocol the denaturation
temperature was 94 C, the
annealing temperature was 58 C,
and the elongation temperature
was 72 C. All StaRT-PCR reagents
had been optimized for the same
PCR conditions. NT and CT PCR
products from the Rapidcycler
were separated and evaluated on
an agarose gel or in the Agilent
2100 bioanalyzer as described
below. Primer pairs used for
amplification in the PTC-100 block
thermal cycler consisted of one
fluorescently labeled primer and
one unlabeled primer for each
gene. These NT and CT PCR products
were separated and analyzed
in an ABI Prism 310 Genetic Analyzer.
Agilent 2100 bioanalyzer
For analysis with the Agilent 2100
bioanalyzer, the DNA 7500
LabChip kit was used. Following
amplification in the Rapidcycler,
1 l of each 10 l PCR reaction
was loaded into a well of a chip
prepared according to protocol
supplied with the DNA 7500
LabChip kit. Briefly, 9 l gel-dye
matrix was loaded into the chip in
one well and the chips were pressurized
for 30 seconds. Two additional
wells were filled with geld
Source:Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
(Date:7/29/2014)... MD (PRWEB) July 29, 2014 ... a vaccine pioneer in the biotechnology industry, announced ... Newport Coast Securities, Inc. to explore financing opportunities ... LAMP-vax technology, a breakthrough vaccine platform with the ... market. The company exclusively licensed the well-established LAMP ...
(Date:7/29/2014)... 29, 2014 DisasterTalk is a texting app ... cell phone masts or other transmitters are down. The app ... away without the need for any Wi-Fi, and can also ... The new app has launched as a housing report ... very high risk of being affected by natural disasters. With ...
(Date:7/29/2014)... The first product launch from start-up ... to robotics. What sets Droidles completely apart from other ... allows them to communicate, share code with each other ... , “The invention is the system,” said Hurley Research ... life of their own, both physically and on the ...
(Date:7/29/2014)... White papers by Clinovo on ... technologies, have been published by leading pharmaceutical publications Data ... line of white papers by Clinovo to have been ... Pharmaceutical Online, and OpenHealthNews. , At the request of ... Marc Desgrousilliers, 'The Five Essentials of Cloud Computing ... Breaking Biology Technology:Immunomic Therapeutics Engages Newport Coast Securities, Inc. 2Immunomic Therapeutics Engages Newport Coast Securities, Inc. 3DisasterTalk App Could Save Lives as Report Reveals 8% of US Homes at Very High Risk of Natural Disaster 2Droidles, the Social Network of Robots and Open Source Platform for the Internet of Things, Launches for Crowd Funding on IndieGoGo.com 2Clinovo White Papers Published in Summer 2014 Editions of Leading Medical Device & Pharmaceutical Publications 2Clinovo White Papers Published in Summer 2014 Editions of Leading Medical Device & Pharmaceutical Publications 3
... marketing for the biotechnology sector, she began to notice a ... product could find the business world to be a much ... required to succeed. , ,"Along the way, I realized that ... make or break a product just as effectively as a ...
... Corp. of suburban Madison will gain early review and ... that Wisconsin Alumni Research Foundation (WARF) has available for ... parties, Promega announced Tuesday. , ,If Promega ... the agreement makes it easier to license the technology ...
... is launching Prospera , an online personnel ... 13,000 human resources professionals, the company announced Tuesday. The ... of concern for human resources staffs: plan, staff, compensate, ... affect a company's bottom line; then it illustrates, step-by-step, ...Cached Biology Technology:Can tech people be business leaders? Upcoming seminar may have the answer 2Can tech people be business leaders? Upcoming seminar may have the answer 3Promega to get early access to WARF technologies under new agreement 2J.J. Keller, Modine Manufacturing, Gustave A. Larson Co., Esker, Firstlogic, Software One 2
(Date:7/28/2014)... The genome of Solanum pennellii , a wild ... an international group of researchers including the labs headed ... UC Davis Department of Plant Biology. The new genome ... , The work, published July 27 in the journal ... and colleagues at Aachen University in Germany. The UC ...
(Date:7/28/2014)... no cure for Alzheimer,s disease and other forms of ... to finding treatment. , University of Washington bioengineers have ... harmful changes of the body,s normal proteins into a ... Parkinson,s, heart disease, Type 2 diabetes and Lou Gehrig,s ... shift from their normal state into an abnormally folded ...
(Date:7/28/2014)... areas of the Earth that are high priorities for ... is particularly vulnerable, as it has the lowest fraction ... any continent in ,refugia, areas of biological diversity ... relatively constant during times of great environmental change. The ... Scandinavia and Scotland. , The biggest refugia are ... Breaking Biology News(10 mins):New protein structure could help treat Alzheimer's, related diseases 2New protein structure could help treat Alzheimer's, related diseases 3Study finds Europe's habitat and wildlife is vulnerable to climate change 2
Hamster Serum; U.S. sourced, ideal for those who seek an uncompromising quality. Low Hemoglobin levels; U.S. sourced, ideal for those who seek an uncompromising quality. Low Hemoglobin levels....
Alkaline Phosphatase (E. coli C75), 100 units. Removes 5'-phosphates from DNA. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzymes, Alkaline Phosphatases....
... Labeling System - dCTP with CyScribe ... the generation and purification of Cy3- ... together for complete cDNA labeling and ... used for performing labeling reactions with ...
... Cy5 Post-Labeling Reactive Dye Pack, ... CyDye fluors specifically optimized for microarray ... 40000 pmol reactive dye per vial, ... to use, individually dispensed, and packed ...Biology Products: