Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
StaRT-PCR was performed using
previously published protocols
with G.E.N.E. system 1a gene
expression kit (Gene Express
National Enterprises, Inc.,
Huntsville, AL). Briefly, a master
mixture containing buffer, MgCl2,
dNTPs, cDNA from normal human
bronchial epithelial cells (lot
17378, American Type Culture Collection,
Rockville, MD), CT mixture
from G.E.N.E. system 1a kit
and Taq polymerase (Promega,
Madison, WI) was prepared.
Aliquots of this mixture were dispensed
into tubes containing genespecific
primers and cycled either
in a Rapidcycler (Idaho Technology,
Inc., Idaho Falls, ID) or PTC-
100 block thermal cycler with
heated lid (MJ Research, Inc.,
Incline Village, NV) for 35 cycles.
In each protocol the denaturation
temperature was 94 C, the
annealing temperature was 58 C,
and the elongation temperature
was 72 C. All StaRT-PCR reagents
had been optimized for the same
PCR conditions. NT and CT PCR
products from the Rapidcycler
were separated and evaluated on
an agarose gel or in the Agilent
2100 bioanalyzer as described
below. Primer pairs used for
amplification in the PTC-100 block
thermal cycler consisted of one
fluorescently labeled primer and
one unlabeled primer for each
gene. These NT and CT PCR products
were separated and analyzed
in an ABI Prism 310 Genetic Analyzer.
Agilent 2100 bioanalyzer
For analysis with the Agilent 2100
bioanalyzer, the DNA 7500
LabChip kit was used. Following
amplification in the Rapidcycler,
1 l of each 10 l PCR reaction
was loaded into a well of a chip
prepared according to protocol
supplied with the DNA 7500
LabChip kit. Briefly, 9 l gel-dye
matrix was loaded into the chip in
one well and the chips were pressurized
for 30 seconds. Two additional
wells were filled with geld
Source:Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
... AlphaBio Centrix, a Nevada-based biotech corporation, announced this week its ... is a term used in programming wearable TheraEX (TM) ... energy field that surrounds all life forms. ... fact, in its basic form, it embodies seven major layers ...
... VILLAHERMOSA, Mexico, March 29, 2012 An advanced mobile bio-containment ... Tabasco to provide bio-surveillance of disease outbreaks. The US-built ... and is the first unit of its kind in ... This lab represents a new generation of technology ...
... Texas, March 28, 2012 Luminex Corporation ... Dr. Amy Altman, vice president of Biodefense will ... Conference , taking place March 27-29, 2012 in Denver, Colorado. ... of Defense,s Joint Program Executive Office for ...Cached Biology Technology:AlphaBio Centrix, LLC Announces a New Discovery for Programming Energetic Patches 2US Lab Deployed to Boost Mexico's Response to Outbreaks of Infectious Disease 2Luminex Corporation to Speak at Joint Department of Defense and Environmental Protection Agency 5th National Bio-Threat Conference 2Luminex Corporation to Speak at Joint Department of Defense and Environmental Protection Agency 5th National Bio-Threat Conference 3Luminex Corporation to Speak at Joint Department of Defense and Environmental Protection Agency 5th National Bio-Threat Conference 4
(Date:1/22/2015)... BellBrook Labs, a leader in high throughput screening ... a TR-FRET (time resolved Forster resonance energy transfer) ... high throughput screening assay for glycosyltransferases and other ... sensitive detection of hundreds of human and microbial ...
(Date:1/22/2015)... OXFORD, Conn. , Jan. 20, 2015 NXT-ID, Inc. (NASDAQ: ... growing mobile commerce market, reports on the recent success of the Wocket™ ... Wocket smart wallet was named as one of the ... of the "5 Best Products Launched At CES So Far" by Newseveryday.com ...
(Date:1/22/2015)... , Jan. 21, 2015 Analyst Report Issued by Small ... Brands report showed that planet-wide, transactions at merchants on the ... the total number of credit, debit, and prepaid cards reached ... were victims of fraudulent card usage in 2012, and credit ... Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... , Feb. 27, 2013 CellTrust Corporation, the recognized ... announced the company,s flagship product CellTrust SecureSMS ® ... Santech Health ,s mobile messaging platform and will demonstrate connection ... HIMSS Conference and Exhibition March 3-7 in ...
... KUALA LUMPUR, Malaysia , Feb. 26, 2013 The governments ... Australia - New Zealand (ANZ) ... border control systems and national ID programs, which bodes well ... Civil Aviation Organization (ICAO) regulation mandating all the countries to ...
... In January 2012, the United States Department of Agriculture ... lunches more nutritious, which included requiring schools to increase ... fruit or vegetable with their purchased lunch. However, children ... a new study scheduled for publication in The ...Cached Biology News:CellTrust SecureSMS PULSE Integrated to Santech Health for Secure Text Messaging of Biometrics and PHI will be Demonstrated at HIMSS 2013 2CellTrust SecureSMS PULSE Integrated to Santech Health for Secure Text Messaging of Biometrics and PHI will be Demonstrated at HIMSS 2013 3Government Emerges As the Biggest Spender on Biometrics in the SEA and ANZ Markets, Finds Frost & Sullivan 2Government Emerges As the Biggest Spender on Biometrics in the SEA and ANZ Markets, Finds Frost & Sullivan 3Government Emerges As the Biggest Spender on Biometrics in the SEA and ANZ Markets, Finds Frost & Sullivan 4Libertarian paternalism and school lunches: Guiding healthier behavior while preserving choices 2
... Assay is an anchorage-independent growth assay in ... stringent assay for detecting malignant transformation of ... with carcinogens or carcinogen inhibitors) are cultured ... for 21-28 days. Following this incubation ...
... CARV II Confocal Imager delivers high resolution ... use and cost effective optical package that ... multi-point confocal scanning, combined with high quantum ... real-time imaging and recording at up to ...
... E-PAGE Gels easier and more efficient by ... several types of pre-cut pre-assembled membrane/filter paper ... 13.5 cm E-PAGE Gels*. The available nitrocellulose ... western transfer of proteins (Figure 1 Table ...
... The DIGIVIEW line of digital x-ray ... radiation imaging device complete with 12 to ... area consists of a photodiode array sensor ... All models are available in low x-ray ...Biology Products: