Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
StaRT-PCR was performed using
previously published protocols
with G.E.N.E. system 1a gene
expression kit (Gene Express
National Enterprises, Inc.,
Huntsville, AL). Briefly, a master
mixture containing buffer, MgCl2,
dNTPs, cDNA from normal human
bronchial epithelial cells (lot
17378, American Type Culture Collection,
Rockville, MD), CT mixture
from G.E.N.E. system 1a kit
and Taq polymerase (Promega,
Madison, WI) was prepared.
Aliquots of this mixture were dispensed
into tubes containing genespecific
primers and cycled either
in a Rapidcycler (Idaho Technology,
Inc., Idaho Falls, ID) or PTC-
100 block thermal cycler with
heated lid (MJ Research, Inc.,
Incline Village, NV) for 35 cycles.
In each protocol the denaturation
temperature was 94 C, the
annealing temperature was 58 C,
and the elongation temperature
was 72 C. All StaRT-PCR reagents
had been optimized for the same
PCR conditions. NT and CT PCR
products from the Rapidcycler
were separated and evaluated on
an agarose gel or in the Agilent
2100 bioanalyzer as described
below. Primer pairs used for
amplification in the PTC-100 block
thermal cycler consisted of one
fluorescently labeled primer and
one unlabeled primer for each
gene. These NT and CT PCR products
were separated and analyzed
in an ABI Prism 310 Genetic Analyzer.
Agilent 2100 bioanalyzer
For analysis with the Agilent 2100
bioanalyzer, the DNA 7500
LabChip kit was used. Following
amplification in the Rapidcycler,
1 l of each 10 l PCR reaction
was loaded into a well of a chip
prepared according to protocol
supplied with the DNA 7500
LabChip kit. Briefly, 9 l gel-dye
matrix was loaded into the chip in
one well and the chips were pressurized
for 30 seconds. Two additional
wells were filled with geld
Source:Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
(Date:12/20/2013)... (PRWEB) December 20, 2013 The December ... the growing need for health outcomes studies will lead ... the already heavily scrutinized drug development budgets. Health Outcomes ... as $500 million and include between 10,000 and 25,000 ... December 2, 2013. Despite these costs, pharmaceutical outcome ...
(Date:12/19/2013)... Sigma-Aldrich Corporation (NASDAQ: SIAL ), a ... Rakesh Sachdev , President and Chief Executive Officer, ... J.P. Morgan Healthcare Conference on Monday, January 13, 2014, ... PM (Pacific). Interested parties may listen via ... at http://investor.sigmaaldrich.com/ . Users can click the "Webcast" ...
(Date:12/19/2013)... NEW YORK , Dec. 19, 2013 /PRNewswire/ ... York -based private equity firm that invests ... announced today the acquisition of Certara, the leading ... software and consulting services to the biopharmaceutical research ... solutions which consist of computer-based models supported by ...
(Date:12/19/2013)... Mich., Dec. 19, 2013 Neogen Corporation (NASDAQ: ... the second quarter of FY 2014, which ended Nov. ... second quarter revenues of $50,737,000. This increase was aided ... year. Year to date, FY 2014 revenues increased 18% ... was $6,207,000, compared to the prior year,s $6,793,000. Adjusted ... Breaking Biology Technology:Pharma Pricing and Market Access News from HealthEconomics.Com for December 20, 2013 2Arsenal Capital Partners Acquires Leading Model-Based Drug Development Company Certara 2Arsenal Capital Partners Acquires Leading Model-Based Drug Development Company Certara 3Arsenal Capital Partners Acquires Leading Model-Based Drug Development Company Certara 4Neogen reports second quarter results 2Neogen reports second quarter results 3Neogen reports second quarter results 4Neogen reports second quarter results 5Neogen reports second quarter results 6
... in the midst of a revolutionary change in diagnostic medicine ... cancer. It is based upon mathematical algorithms that can accurately ... a veteran Wall Street Securities Analyst in a report about ... Board: HDVY ), located in Savannah, GA., has ...
... Intrinsic Bioprobes Inc. ("IBI") today announced the ... for in-vitro diagnostic testing. The assays for beta-2-microglobulin, ... on IBI,s MASSAY® platform at the CLIA-certified and ... of the Institute of Genomics Medicine, UMDNJ-New Jersey ...
... Clontech Laboratories, Inc., a wholly-owned subsidiary of ... the iDimerize inducible dimerization systems . Based ... developed by ARIAD Pharmaceuticals, Inc. (Nasdaq: ... control of protein-protein interactions using specific cell-permeable chemical ...Cached Biology Technology:Veteran Wall Street Securities Analyst Reports on Health Discovery Corporation 2Intrinsic Bioprobes Launches Three New CLIA Approved Mass Spectrometry-Based Tests for Proteins Implicated in Kidney Disease and Renal Failure 2Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 2Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 3Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 4
(Date:12/20/2013)... funded by the National Science Foundation (NSF) and led ... will attempt to characterize the complex dynamics of mercury ... ecosystems. , Mercury is a neurotoxic element found naturally ... fuel combustion, mining, smelting and waste combustion have increased ... to five over the last 150 years. That increased ...
(Date:12/20/2013)... percent of the major global cereal crops rice, ... possible yields in farmers, fields, according to University of ... . These findings raise concerns about efforts to increase ... these crops have recently decreased or plateaued. Future projections ... on a constant increase in yield, a trend that ...
(Date:12/20/2013)... Morten Trillingsgaard Ven, Department of Molecular Biology and Genetics ... article on microRNA-128 just published in Science . ... control the amount of microRNA-128 in specific neurons that ... kept down in these neurons in neonatal mice, it ... "It leads to a higher level of activity, which ... Breaking Biology News(10 mins):New DRI project aimed at understanding Mercury dynamics in the Arctic tundra 2UNL research raises concerns about global crop projections 2New research provides insight into epilepsy 2
Practical tool for easy, effective sealing of 0.2ml PCR tubes and plates using cap strips....
... Distributed exclusively by BioLegend, the Quansys ... for multiplex analysis of cytokines/chemokines for human, ... Cytokine Array is a fully quantitative ELISA-based ... been absorbed to each well of a ...
The strip cap tool is a used for properly seating 8- and 12-cap strips in microplates or strip tubes. A grooved channel on one side is designed to seat domed caps, while the flat edge on the opposite...
... The expertise that made the original Gene ... electroporation system available has been applied in the ... circuitry. The modular design of the Gene Pulser ... the accessory that has the widest range of ...Biology Products: