Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
StaRT-PCR was performed using
previously published protocols
with G.E.N.E. system 1a gene
expression kit (Gene Express
National Enterprises, Inc.,
Huntsville, AL). Briefly, a master
mixture containing buffer, MgCl2,
dNTPs, cDNA from normal human
bronchial epithelial cells (lot
17378, American Type Culture Collection,
Rockville, MD), CT mixture
from G.E.N.E. system 1a kit
and Taq polymerase (Promega,
Madison, WI) was prepared.
Aliquots of this mixture were dispensed
into tubes containing genespecific
primers and cycled either
in a Rapidcycler (Idaho Technology,
Inc., Idaho Falls, ID) or PTC-
100 block thermal cycler with
heated lid (MJ Research, Inc.,
Incline Village, NV) for 35 cycles.
In each protocol the denaturation
temperature was 94 C, the
annealing temperature was 58 C,
and the elongation temperature
was 72 C. All StaRT-PCR reagents
had been optimized for the same
PCR conditions. NT and CT PCR
products from the Rapidcycler
were separated and evaluated on
an agarose gel or in the Agilent
2100 bioanalyzer as described
below. Primer pairs used for
amplification in the PTC-100 block
thermal cycler consisted of one
fluorescently labeled primer and
one unlabeled primer for each
gene. These NT and CT PCR products
were separated and analyzed
in an ABI Prism 310 Genetic Analyzer.
Agilent 2100 bioanalyzer
For analysis with the Agilent 2100
bioanalyzer, the DNA 7500
LabChip kit was used. Following
amplification in the Rapidcycler,
1 l of each 10 l PCR reaction
was loaded into a well of a chip
prepared according to protocol
supplied with the DNA 7500
LabChip kit. Briefly, 9 l gel-dye
matrix was loaded into the chip in
one well and the chips were pressurized
for 30 seconds. Two additional
wells were filled with geld
Source:Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
(Date:8/4/2015)... , Aug. 4, 2015 Blueprint Medicines (NASDAQ: ... highly selective kinase drugs for genomically defined diseases, today ... will present a company overview at the 2015 Wedbush ... 1:20 p.m. EDT. A live webcast of ... section of the Blueprint Medicines website at www.blueprintmedicines.com ...
(Date:8/4/2015)... 4, 2015 /PRNewswire/ -- Sinovac Biotech Ltd. (Nasdaq: SVA ... China , today announced that it will ... ended June 30, 2015, after market close on Wednesday, ... on Thursday, August 13, 2015, at 8:00 a.m. EDT ... Time) to review the Company,s financial results and provide ...
(Date:8/3/2015)... 2015 MiMedx Group, Inc. (NASDAQ: MDXG ), ... and patent-protected processes to develop and market advanced products ... Sports Medicine, Ophthalmic and Dental sectors of healthcare, announced ... Annual Canaccord|Genuity Growth Conference in Boston, ... CEO, William C. Taylor , President and COO, ...
(Date:8/3/2015)... 2015 Aethlon Medical, Inc. (Nasdaq: AEMD ), ... life-threatening diseases, today announced that it will host its ... on Thursday, August 13, 2015 at 4:30 p.m. Eastern ... the release of fiscal 2016 first quarter results. A ... two hours after the call at the Investor Relations ... Breaking Biology Technology:Sinovac to Host Conference Call to Report Second Quarter 2015 Unaudited Financial Results 2MiMedx To Present At 35th Annual Canaccord|Genuity Growth Conference 2Aethlon Medical To Host First Quarter Fiscal 2016 Investor Call on August 13, 2015 2
... 11, 2011 /PRNewswire-Asia/ -- Chinmax Medical Systems Inc., a ... regarding the progress of its lawsuit against Inverness Medical ... According to the Complaint, Inverness Medical Beijing ... the same to Chinmax customers during the period when ...
... SAN DIEGO, April 11, 2011 MabVax Therapeutics, Inc., ... the development of vaccine and antibody based therapies to ... announces that it has received the Phase 2 portion ... the National Cancer Institute (NCI) under the agency,s Streamlined ...
... VIGILAIR Systems, an industry leader in Ultraviolet Germicidal ... and first of its kind hospital study that demonstrated ... with the application of VIGILAIR® air cleaning technology in ... published article , Effect of enhanced ultraviolet germicidal irradiation ...Cached Biology Technology:Beijing Chaoyang District Court Grants Investigation Letters Against Inverness Medical Beijing, A Subsidiary of Alere Inc. 2MabVax Therapeutics Receives $1.8 Million in a Phase 2 Small Business Innovation Research Program Award From the National Cancer Institute 2MabVax Therapeutics Receives $1.8 Million in a Phase 2 Small Business Innovation Research Program Award From the National Cancer Institute 3Newly Published Study Shows Dramatic Reduction in Hospital Acquired Infections 2
(Date:7/8/2015)... , July 8, 2015 The ... as this summer,s must have products such as wearables, smart ... as popularity for biometrics based devices continues to rapidly grow. ... Inc. (NASDAQ: NXTD ), Google Inc. (NASDAQ: GOOG ... FIT ), GoPro, Inc. (NASDAQ: GPRO ) and ...
(Date:7/7/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... commerce market announces a revised version of one of its first ... air on CNBC in New York , ... San Francisco metro areas. ... ability to replace all the cards in your wallet and keep ...
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ... Breaking Biology News(10 mins):Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 2Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 4Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 5Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 6Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 2Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 3FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... levels of carbon dioxide in the atmosphere are a ... The lack of atmospheric carbon dioxide (CO 2 ) ... their aquatic relatives, microalgae. For plants and microalgae, ... their photosynthesis process that, along with sunlight, manufactures sugars ...
... University of Wisconsin, USA, report in the journal ... human embryonic stem cells of a type of cell ... new possibilities for both basic and clinical research. ... are responsible for making myelin in the central nervous ...
... America,s biological field stations have long been home to ... them important places for serendipitous discoveries in the biological ... the April issue of the journal BioScience , ... Term Ecological Research (LTER) Network and other groups state ...Cached Biology News:ISU researcher identifies protein that concentrates carbon dioxide in algae 2Human ES cells progress slowly in myelin's direction 2Field stations foster serendipitous discoveries in environmental, biological sciences 2Field stations foster serendipitous discoveries in environmental, biological sciences 3
Mouse monoclonal [2Q2147] to Aequorin ( Abpromise for all tested applications)....
Phosphotyrosine IgG mouse monoclonal antibody: Alkaline Phosphatase conjugate...
... mixed TAMRA isomers are predominantly used for ... preferred for labeling peptides and nucleotides because ... that is often required in the conjugation ... 6-TAMRA for labeling peptides and proteins. 6-TAMRA ...Biology Products: