Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR
PCR reagents for over 400
genes are now available commercially
through Gene Express
National Enterprises, Inc.*
for an additional 2000 genes are in
production. In a blinded inter-laboratory
study of common samples,
this method provided excellent
reproducibility (manuscript in
preparation). Thus, all laboratories
using a common mixture of
internal standards for StaRT-PCR
will be able to enter gene expression
data into a common gene
expression databank. Such a databank
has been established at
StaRT-PCR reactions include
cDNA from a cell or tissue sample
of interest and a standardized mixture
of competitive template internal
controls for each gene to be
measured. Quantitation is based
on a ratio of each endogenous
gene (referred to as native template
[NT]) PCR product to the
corresponding shorter internal
standard competitive template
(CT) product. Until recently, the
primary method used to size, separate
and detect NT and CT StaRTPCR
products has been agarose
gel electrophoresis. This method
is available to most laboratories
without the purchase of additional
equipment. However, recently
available alternative methods for
electrophoresis, including the Agilent
2100 bioanalyzer have the
potential to markedly increase
throughput and reproducibility.
Additionally, if StaRT-PCR is to
have broad application, it is
important to determine whether
electrophoresis and analysis of StaRT-PCR products with different
instruments will provide comparable
data. This question was
addressed by comparing StaRTPCR
expression data for 15 genes
obtained following electrophoretic
separation of PCR products by
either the usual agarose gel, the
Agilent 2100 bioanalyzer (Agilent
Technologies, Inc., Palo Alto, CA),
or the ABI Prism 310 Genetic Analyzer
(Applied Biosystems, Foster
Page: All 1 2 3 4 5 6 7 Related biology technology :1
. Mx4000 Multiplex Quantitative PCR System*,PPP2
. Improved Quantitative Selectivity of Clenbuterol in Human Urine using
High Resolution on the TSQ Quantum Mass Spectrometer3
. The LightCycler is the only System for Quantitative PCR that is as Flexible
. Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection5
. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System6
. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A7
. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A8
. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A9
. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System10
. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma11
. Quantitative Analysis of the Cell Cycle in Imaging Applications
(Date:9/19/2014)... Paul, Minnesota (PRWEB) September 19, 2014 ... from related food industries will gather October 5–8, ... at the Rhode Island Convention Center in Providence, ... will feature the work of food scientists, chemists, ... quality control, and food production management. To date, ...
(Date:9/18/2014)... HealthTronics, Inc ., a ... products and services, announced that a poster related ... versus external beam radiotherapy (EBRT) is being presented ... Association (MAAUA) Annual Meeting in Baltimore, Maryland September ... Cryosurgery and External Beam Radiation as Treatments for ...
(Date:9/18/2014)... , September 18, 2014 ... ( http://www.researchandmarkets.com/research/czvg3s/biocompatible ) has announced the ... - Global Strategic Business Report" ... http://photos.prnewswire.com/prnh/20130307/600769) ,This report analyzes the worldwide ... Million by the following Product Segments: ...
(Date:9/18/2014)... Research and Markets has announced ... Report 2014" report to their offering. ... a professional and in-depth study on the current state ... report provides a basic overview of the industry including ... albumin market analysis is provided for the international markets ... Breaking Biology Technology:Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3HealthTronics Announces Data Comparing Cryotherapy Versus Radiation for Treating Prostate Cancer Poster Presented at the American Urological Association Regional Meeting 2Biocompatible Materials - Global Strategic Business Report 2014 2Biocompatible Materials - Global Strategic Business Report 2014 3Global Human Albumin Industry Report 2014 2Global Human Albumin Industry Report 2014 3
... NVAX ) today announced that the Company will ... II human clinical trial in,healthy adult subjects of Novavax,s seasonal ... 11, 2008. , The ... Officer,Dr. Rahul Singhvi and other members of senior management, including ...
... ORLANDO, Fla., Dec. 8 Building on its ... Health today,launched the Pyxis(R) MedStation(R) 4000 and an ... actionable,recommendations to help improve medication safety. , ... 4000 automated medication dispensing system,offers new features that ...
... 8 Interleukin Genetics, Inc. (Amex: ILI ), ... as Vice President, Marketing. Mr. Walker will be responsible ... reporting to the Chief Executive Officer, Lewis H. Bender. ... well as 20 years of industry-related experience. , ...Cached Biology Technology:Novavax to Present Seasonal Influenza Data Results in an Investor Conference Call on December 11, 2008 2Cardinal Health Launches Pyxis(R) MedStation(R) 4000; Introduces Industry-First Service to Interpret Dispensing and Infusion Data 2Cardinal Health Launches Pyxis(R) MedStation(R) 4000; Introduces Industry-First Service to Interpret Dispensing and Infusion Data 3Interleukin Genetics Appoints Todd Anthony Walker as Vice President, Marketing 2Interleukin Genetics Appoints Todd Anthony Walker as Vice President, Marketing 3
(Date:9/19/2014)... unveiled today by researchers from several Harvard University ... both experienced and aspiring researchers with the intellectual ... robots made from soft, flexible materials. , With ... and other advances in manufacturing technology, soft robotics ... principles drawn from conventional rigid robot design, but ...
(Date:9/19/2014)... fairness did not evolve for the sake of fairness ... of continued cooperation, so say Frans de Waal, PhD, ... about inequity aversion (IA), which is defined as a ... in Science . , Their conclusion comes ... to address their hypothesis that it is the evolution ...
(Date:9/19/2014)... new study shows that legume plants regulate their ... molecules that are transmitted through the plant structure ... number of bacteria-holding nodules in the roots. This ... National Institute for Basic Biology, the Graduate University ... for Sustainable Resource Science in Japan. , ... Breaking Biology News(10 mins):Soft robotics 'toolkit' features everything a robot-maker needs 2Evolution of responses to (un)fairness 2Evolution of responses to (un)fairness 3For legume plants, a new route from shoot to root 2
... Target Retrieval Solution, High pH This ... target retrievel prior to immunohistochemical staining procedures. ... tissue sections mounted on glass slides. For ... 9.9, is especially effective. It should be ...
... very potent Rnase free Ribonuclease ... a broad range of temperatures ... does not inhibit SP6, T7, ... and M-MLV reverse transcriptase or ...
... Dako Target Retrieval Solution, pH 9 ... Tris/EDTA buffer, pH 9, intended for heat-induced ... It is well-suited for use on formalin-fixed, ... Compared with 0.01 mol/L citrate buffer, pH ...
... SeMet White to off-white solid. Increases ... by HPLC. Soluble in H 2 O. ... to use. RTECS EK7713840, CAS 3211-76-5, M.W. ... 13, 8515.Jornot, L., and Junod, A.F. 1995. ...Biology Products: