Navigation Links
Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR

PCR reagents for over 400 genes are now available commercially through Gene Express National Enterprises, Inc.* (www.genexnat.com). Reagents for an additional 2000 genes are in production. In a blinded inter-laboratory study of common samples, this method provided excellent reproducibility (manuscript in preparation). Thus, all laboratories using a common mixture of internal standards for StaRT-PCR will be able to enter gene expression data into a common gene expression databank. Such a databank has been established at G.E.N.E., Inc.
StaRT-PCR reactions include cDNA from a cell or tissue sample of interest and a standardized mixture of competitive template internal controls for each gene to be measured. Quantitation is based on a ratio of each endogenous gene (referred to as native template [NT]) PCR product to the corresponding shorter internal standard competitive template (CT) product. Until recently, the primary method used to size, separate and detect NT and CT StaRTPCR products has been agarose gel electrophoresis. This method is available to most laboratories without the purchase of additional equipment. However, recently available alternative methods for electrophoresis, including the Agilent 2100 bioanalyzer have the potential to markedly increase throughput and reproducibility. Additionally, if StaRT-PCR is to have broad application, it is important to determine whether electrophoresis and analysis of StaRT-PCR products with different instruments will provide comparable data. This question was addressed by comparing StaRTPCR expression data for 15 genes obtained following electrophoretic separation of PCR products by either the usual agarose gel, the Agilent 2100 bioanalyzer (Agilent Technologies, Inc., Palo Alto, CA), or the ABI Prism 310 Genetic Analyzer (Applied Biosystems, Foster City, CA).
S
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/10/2014)... 10, 2014 Product and process ... low abundance and are often “lost in the ... , Join presenters Dr. Rowel Tobias, Senior Scientist, ... Anders, Head of Quality at Nanotherapeutics, Inc., to ... can speed detection and quantitation while achieving the ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Terascala, the ... announced that Alan Swahn, a former vice president of ... team as vice president of marketing. Terascala’s software when ... and NetApp create the highest performance and most reliable ... appointed to guide Terascala’s channel expansion and broaden its ...
(Date:7/10/2014)... Angeles, CA (PRWEB) July 10, 2014 ... and Bioanalytical techniques during 18-20 August, 2014 at Double ... critically review the recent developments in Analytical & Bioanalytical ... the globe. , Speaking on this occasion, Dr. Srinubabu ... Analytica Acta conference is a remarkable one in ...
(Date:7/10/2014)... , July 10, 2014  Franciscan St. ... of capnography for respiratory monitoring outside the ... healthcare leaders in embracing state-of-the-art patient safety ... patients are breathing and can alert medical ... measuring the amount of carbon dioxide the ...
Breaking Biology Technology:Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Terascala Expands Executive Team 2Terascala Expands Executive Team 3A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2
... have pleaded guilty in Milwaukee to charges of criminal ... $6 million in counterfeit Rockwell Automation computer ... Alice Fisher for the Criminal Division and U.S. Attorney ... announced that Robert Koster of Jonesboro, Ark. pleaded ...
... of digital everything, print still captures the imagination of consumers ... , ,There's no clearer example than Blurb . I ... was no more than a brief PowerPoint deck. ... afternoon and Eileen unwound a vision for consumer-generated books that ...
... Jay Kouba, a 25-year veteran of the chemical and petroleum ... Energy Systems . , ,Virent, which recently entered into an ... Oil Co., is developing technologies that allow the renewable replacement ... of Metabolix, Inc. , a publicly traded Massachusetts bioscience ...
Cached Biology Technology:Two convicted of selling $6 million in counterfeit Rockwell software on eBay 2Blurb shows that print still captures imaginations 2Blurb shows that print still captures imaginations 3
(Date:7/11/2014)... University of Chicago is creating a new professorship ... the University,s Institute for Molecular Engineering and the ... donation from the Millicent and Eugene Bell Foundation., ... reside within the Institute for Molecular Engineering. That ... project at the MBL,s Eugene Bell Center for ...
(Date:7/11/2014)... Shenzhen, China Researchers from Salk Institute for Biological ... time evaluated the safety and reliability of the ... a new method, TALEN-HDAdV, which could significantly increased ... (hiPSC). This study published online in Cell ... for stem cell-based gene therapy. , The combination ...
(Date:7/11/2014)... 2014 The increased risk of kidney injury related ... fluids reflects the mass of HES molecules, according to ... journal of the International Anesthesia Research Society (IARS). , ... effect of HES on cultured human renal proximal tubule ... Wunder and colleagues of University Hospital Wrzburg, Austria. ...
Breaking Biology News(10 mins):New professorship in tissue engineering links molecular engineering, marine biology 2A new genome editing method brings the possibility of gene therapies closer to reality 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... . . . With a network ... seismic activity, traffic, bacteria on beaches, water levels and ... to expand. Sensorpedia, developed three years ago by Oak ... first responders, individuals and communities with online sensor data ...
... ARBOR, Mich. Why do some diseases affect only ... of Michigan have found neural tissue contains imbalanced levels ... a debilitating childhood movement disorder. Known as ... movements or abnormal postures. It,s caused by a mutation ...
... 1. Discover the latest in Earth and Space Sciences ... findings in the Earth and space sciences in Foz do ... the meeting, which takes place from 8 to 12 August, ... 2010 Haiti and Chile earthquakes, extreme weather events in the ...
Cached Biology News:Story tips from the US Department of Energy's Oak Ridge National Laboratory -- June 2010 2Neural tissue contains imbalanced levels of proteins, U-M study finds 22010 Meeting of the Americas, Brazil: News media registration open 22010 Meeting of the Americas, Brazil: News media registration open 32010 Meeting of the Americas, Brazil: News media registration open 4
Mouse monoclonal antibody raised against a partial recombinant CAPG. NCBI Entrez Gene ID = CAPG...
Mouse monoclonal antibody raised against a partial recombinant PDE2A. NCBI Entrez Gene ID = PDE2A...
...
... sample is a complex protein sample, ... when used with ReadyPrep 2-D starter ... sample can be used as a ... and protocol before using more difficult ...
Biology Products: