Navigation Links
Quantitative end-point RT-PCR gene,,,expression measurement using the,,,Agilent 2100 Bioanalyzer and,,,standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer can be used in combination with Standardized RT (StaRT)-PCR reagents from Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville, AL, USA) to obtain highly reproducible, standardized gene expression measurement with RT-PCR. The internal standards used in StaRT-PCR control for variable loading of PCR products into electrophoresis channel, and allow quantitative gene expression measurement at the endpoint of PCR, without real-time analysis. Gene expression measurement of 15 genes was more reproducible with the Agilent 2100 bioanalyzer than with the ABI Prism 310 Genetic Analyzer or agarose gel electrophoresis methods. The mean coefficient of variance (CV) for these three methods was 0.26, 0.39 and 0.50, respectively.
We combined the Agilent 2100 bioanalyzer with a quantitative method for standardizing gene expression, Standardized RT (StaRT)-PCR (Willey et al, Am. J. Resp. Cell Mol. Biol., 19, 6, 1998), to produce a sensitive, reproducible method for quantitative end-point PCR measurement of gene expression. The Agilent 2100 system markedly simplifies electrophoresis. There is no need to prepare agarose gels and, following electrophoresis, immediate results are provided by PCR product quantification software. The internal standards used in StaRTPCR control for variable loading of PCR products into electrophoresis channels, and allow quantitative gene expression measurement at the end point of PCR, without real-time analysis. StaRTPCR used with agarose gel electrophoresis has been validated in independent studies (Loitsch et al, Clinical Chemistry, 45, 619, 1999; Allen et al, Am. J. Resp. Cell Mol. Biol., 21, 693, 1999; Mollerup et al, Cancer Research, 59, 3317, 1999). StaRT-
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/10/2014)... , July 10, 2014 Research ... addition of the "International Photonic Integrated Circuit ... Forecasts to 2019" report to their offering. ... The concept of photonic integration traces its ... century. The promise of photonic integration went unexplored ...
(Date:7/10/2014)... to the International Atomic Energy Agency (IAEA) the ... acquiring sufficient quantities of plutonium or highly enriched ... device. The IAEA also notes that most cases ... which can be challenging to detect with most ... appearing this week in the Journal of ...
(Date:7/10/2014)... WASHINGTON D.C., June 17, 2014 Using microscopic ... of specific gases, researchers at MIT,s Quantum Photonics ... detection levels in the parts-per-billion range. Optical sensors ... to their high signal-to-noise ratio, compact, lightweight nature, ... optical gas sensors had been developed before, the ...
(Date:7/10/2014)... Understanding the basis of psychiatric disorders has been extremely ... increase risk but are insufficient to cause disease. Now ... Cell Press journal Cell Stem Cell describe ... genetic risks interact with other risk factors or environmental ... Their research pinpoints a genetic variant that may predispose ...
Breaking Biology Technology:International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2Sensitive detection method may help impede illicit nuclear trafficking 2Swell new sensors 2New strategy could uncover genes at the root of psychiatric illnesses 2
... to build a business from scratch and sell it for a ... we wanted to speed it up so the entire thing from ... , ,In a few rare cases, thats exactly what happened for ... what my father had told me for years: Patience is a ...
... A Wisconsin Company that manufactures a state-of-the-art homeland ... the top 100 technological innovations of 2003 ... of What's New Awards honored Oshkosh Truck Corporations ... in the Engineering category, describing the new command-and-communications vehicle ...
... to write about weblogs - until now. Truth be told - ... much for bandwagons. If everyone is going right, I'll move left ... first take on blogging was that it was just another technology-enabled ... that any other fool could read it. Of course, there was, ...
Cached Biology Technology:An Overnight Business Success Takes 20 Years 2An Overnight Business Success Takes 20 Years 3Oshkoshs Pierce Security Vehicle Ranked in top 100 Technological Innovations for 2003 2The Blog Nation 2The Blog Nation 3
(Date:7/10/2014)... cities in New Mexico, nearly every public golf course ... precious potable water supplies. Across the U.S. Southwest as ... receive treated effluent. Reusing the effluent increases the sustainability ... homeowners alike fertilize their lawns during the growing season. ... Mexico State University turfgrass expert has a new vision ...
(Date:7/10/2014)... July 2, 2014  Unisys Corporation,s (NYSE: UIS ... Nederland N.V., today announced that it has been selected by ... manage a new Basic Provision Biometrics solution for penitentiaries across ... won the contract with a solution based on its open ... term of the contract is up to seven years, with ...
(Date:7/10/2014)... GOTHENBURG, Sweden , July 7, 2014 ... won its first major design win (DW). A Chinese Top ... with the planned start of mass production in October 2014. ... with a Chinese Top 5 smartphone OEM, which has a ... 2014 and a target sales volume for this phone of ...
Breaking Biology News(10 mins):'Tailored' water -- the latest in lawn care 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3FPC Wins DW for 1021 Touch Sensor for Flagship Smartphone from Chinese Top 5 Smartphone OEM 2
... working with collaborators in Germany, France and Korea have ... of twisting or torque in a metallic nanowire. This ... enable measurements of the untwisting of DNA and have ... Spin-induced torque is central to understanding experiments, from ...
... CINCINNATI Two proteins interact in a previously unknown ... studies looking for the causes of defective organs in ... at Cincinnati Children,s Hospital Medical Center. Reporting their ... , the researchers said the mechanism coordinates cell identity ...
... inventors designing and manufacturing biomedical sensors, healthcare and its ... Come learn what to expect from this new and ... free seminar set for Nov. 6, 2008, from 4:45-6 ... Engineering (ECE) Building, 141 Warren St, at the northwest ...
Cached Biology News:Detecting tiny twists with a nanomachine 2New regulatory mechanism discovered for cell identify and behavior in forming organs 2New regulatory mechanism discovered for cell identify and behavior in forming organs 3NJIT seminar set for Nov. 6 to focus on bioelectronics -- emerging research area 2
Request Info...
...
Mouse GM CSF Construction Kit provides antigen affinity purified polyclonal capture and tracer antibodies, and antigen standard for development of approximately ten microplate assays....
... Inventory Storage Racks for Standard 3" ... fit all makes and models of ... of high quality stainless steel and ... stainless steel or plastic boxes. ...
Biology Products: