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Quantitative Measurement of Cell Proliferation,,,Using the BrdU ELISA: A Comparison,,,Between Colorimetric and Chemiluminescent Detection

test is the method of choice because it enables linear results to be obtained over a wide interval. When, on the other hand, proliferation measurements are made on only a few or even just one cell line over a limited period of time, the more easyto- use colorimetric test can be employed. Using a colorimetric assay one must always consider that this test has only a limited linear range. In contrast to numerous other ELISA kits, however, when the linear range of the BrdU ELISA is exceeded, it is not possible to just dilute the sample because the latter is a cellular ELISA in which the labeled, cultivated cells produce the signals. In such cases, the only alternative is to perform a completely new experiment with a shorter labeling time. Reducing the cell number in order to bring the signal to the linear range is not feasible because this would change the overall proliferation behavior of the cell population in vitro.

These observations are of practical relevance. Not all laboratories performing routine DNA synthesis determinations possess the apparatus required for luminescence measurements because it is considerably more expensive than systems for measuring absorption. The experiments described here may help in this decision-making process.




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