Navigation Links
Quantitative Measurement of Cell Proliferation,,,Using the BrdU ELISA: A Comparison,,,Between Colorimetric and Chemiluminescent Detection

etric data as increases of merely 16 % and 20 % were recorded. However, the extent of the cell proliferation already leads to values in the non-linear region. Use of the chemiluminescence technique results in different sets of data. In this case, depending on the cell line, growth advantages of 55 % or 63 % are achieved by supplementation of the medium. The alternative, to choose the linear region of the colorimetric test for the experiments, is in general not very practicable since the problems investigated with these test systems usually involve examination of the effects of several parameters in a single experiment. The positive or negative changes in the proliferation index or incorporation of BrdU may vary from parameter to parameter. Thus, it is often necessary to repeat one or more of the experiments, in some circumstances with differing cell numbers or labeling times.

The colorimetric test can be employed without difficulty in cell systems and experiments in which short labeling times are chosen or in which changes in proliferation are measured at the end of a growth curve, near confluence. This is shown by the results with comparatively slowly growing fibroblasts (ZF 07) and the endothelial cells (HUVEC). In these cases, the two BrdU systems provide almost the same information, namely inhibition of cell division of fibroblasts upon use of cholera toxin or the advantage of serum-free endothelial medium in comparison with a standard cell culture medium. The absorption values of the colorimetric BrdU test are in the linear region; thus the differences in BrdU incorporation can be reliably recorded by instruments and are comparable with those from the chemiluminescence technique.

When measurements have to be made on several differently proliferating cells under different conditions in one experiment, the chemiluminescence
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
5. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/29/2015)... , July 30, 2015 ... la santé, publie ses résultats pour le premier ... commente les résultats. Visionner l,interview ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Au sommaire de ... - Moteurs de croissance - ...
(Date:7/29/2015)... MONTREAL , July 29, 2015 /PRNewswire/ - BioAmber Inc. ... Laurin , the Company,s Chief Financial Officer has resigned.  ... former Chief Financial Officer who retired in December 2014, ... search for a permanent successor.     Andy ... Mr. Laurin and ensure a smooth transition.  Andy was ...
(Date:7/29/2015)... 2015 Research and ... of the "Global Biosimilars Market, 2015 - ... The Global Biosimilars Market, 2015 - 2025, report ... biosimilars market. With the blockbuster biologics losing patent ... biosimilars are being viewed as viable substitutes to ...
(Date:7/29/2015)... 29, 2015  Pfenex Inc. (NYSE MKT: PFNX) announced ... be released on Thursday, August 13, 2015, before the ... management will host a conference call to discuss the ... release outlining the financial results and business update will ... Please call 1-866-376-8058 (US) or 1-412-542-4131 (international) and reference ...
Breaking Biology Technology:BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 2Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 3Pfenex To Report Second Quarter 2015 Results and Provide Business Update on Thursday, August 13, 2015 2
... States Health Alliance , a regional health care system ... Milwaukee-based Johnson Controls, Inc. , for system-wide installation ... will be installed throughout the health care system, including ... a variety of voice and data services. , ,Mountain ...
... pictured here in 2001 in his Primate Center lab. Source: ... anatomy professor James Thomson attempted to separate hype ... cell research, which in some cases uses tissue from human ... 200 at the UW Memorial Union as part of the ...
... a job offer from one of the employers youve been tangoing ... Think of it as getting an interview but squared. It means ... when we are wanted (not to mention the prospect of getting ... our good judgment. , ,Taking a job is not like going ...
Cached Biology Technology:Tennessee health care system to install Johnson Controls wireless tech 2UW stem cell guru outlines scientific and political future 2UW stem cell guru outlines scientific and political future 3Ten red flags in your Midwest technology offer letter 2Ten red flags in your Midwest technology offer letter 3Ten red flags in your Midwest technology offer letter 4Ten red flags in your Midwest technology offer letter 5
(Date:7/31/2015)... , Jul. 31, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market ... it has filed provisional patent 62/198989 for ... invention highlights a method to advance crypto-currencies such as ... introducing a common, uniform way to manage all payments.  ...
(Date:7/23/2015)... 23, 2015 Aware, Inc. (NASDAQ: AWRE ), ... financial results for its second quarter ended June 30, 2015.  ... was $4.5 million, a decrease of 33% compared to $6.8 ... the second quarter of 2015 was $0.3 million, or $0.01 ... per diluted share, in the same period a year ago.  ...
(Date:7/20/2015)...  Acuity Market Intelligence,s latest research "The Global ... and Privacy" forecasts that between 2014 and 2020 ... downloaded to smart mobile devices by 2.2 billion ... projected to generate more than $67.9 billion in ... period.    "Biometrics is at the ...
Breaking Biology News(10 mins):NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6Biometrics on Smart Mobile Devices to Redefine Digital Identity with 12.9 Billion Biometric App Downloads between 2014 and 2020 2
... The Howard Hughes Medical Research Institute today announced that ... among 50 scientists nationwide to be appointed HHMI Early ... associate member of the Center,s Basic Sciences Division, and ... Center,s Human Biology and Public Health Sciences divisions, each ...
... tiny semipalmated sandpipers embark on their annual odyssey from the ... set out on one of the world,s longest migrations. On ... of Fundy on the Canadian east coast, where they spend ... which have some of the highest levels of n-3 fatty ...
... stocking has on aquatic insects in mountain lakes can ... a study completed by U.S. Forest Service and University ... current online issue of the journal Freshwater Biology where ... management practices now in use in California mountain lakes ...
Cached Biology News:Two Hutchinson Center researchers named HHMI Early Career Scientists 2Quails get super fit by simply eating omega-3 diet 2Study assesses impact of fish stocking on aquatic insects 2
... for isolation and affinity purification of ... peptides. Hydrophobic, monodisperse magnetic particles (2.8 ... groups, further surface activation is not ... with optimal orientation for affinity purification ...
ZO-2 (H-110)...
p-Bcl-2 (Thr 74)...
Derlin-2 (C-18)...
Biology Products: