Navigation Links
Quantitative Measurement of Cell Proliferation,,,Using the BrdU ELISA: A Comparison,,,Between Colorimetric and Chemiluminescent Detection

e examined the colorimetric and the chemiluminescence variants of the BrdU ELISA (Roche Molecular Biochemicals) in a series of parallel experiments.

Materials and Methods

Details of our special cell culture system and a short review on current measurement techniques for cell proliferation has been published [1-4]. We have investigated the proliferation of two distinct human prostatic carcinoma cell lines, P1/289 and P1/373 previously established in our laboratory.

In preliminary experiments, we had already defined the relevant cultivation and labeling intervals as well as the optimal cell concentrations for these cells. We have now investigated the influence of growth factors (epidermal growth factor, EGF) as well as the effects of insulin (Ins) and hydrocortisone (HC) on DNA synthesis in the cell lines P1/289 and P1/373. For this experiment, 104 cells were seeded in standard 96-well microtiter plates in a volume of 200 l medium/well and cultivated in complete medium (DMEM/F12, Life Technologies) containing 10 % fetal calf serum (FCS) supplemented with EGF (10 ng/ml), hydrocortisone (10 ng/ml), cholera toxin (CT, 10 ng/ml), and insulin (4 g/ml). To examine the effects of the additives (DMEM/F12+) on cell proliferation, the cell lines were also cultivated in parallel in their absence (DMEM/F12-).

A second application concerned another cell culture model: To prevent the outgrowth of fibroblasts when establishing cell cultures from heterogeneous tissue samples, cultivation of epithelial cells in the presence of cholera toxin is recommended. When added to normal cell culture medium, this toxin selectively suppresses the growth of fibroblasts. Accordingly, we cultured human fibroblasts obtained from explants (ZF 07) in microtiter plates. The rate o
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
5. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/10/2014)... July 10, 2014 Women in ... access to an additional family planning option. Sayana® ... contraception at all levels of the health system ... of a widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone ... Faso is the first of four African countries ...
(Date:7/10/2014)... July 10, 2014 Unraveling life’s mysteries ... The Archer Family purchased a DNA test ... but ended up with a lifetime of memories, sharing, ... the family members’ DNA genuinely brought the family closer ... search deeper into genetic history and prior to taking ...
(Date:7/10/2014)... Ind. , July 10, 2014 /PRNewswire/ ... its use of capnography for respiratory monitoring ... group of healthcare leaders in embracing state-of-the-art ... how effectively patients are breathing and can ... occurs. By measuring the amount of carbon ...
(Date:7/10/2014)... 10, 2014   Ceres, Inc . (Nasdaq: ... announced today financial results for the three months ended ... business. Ceres reported that the company and ... this growing season in Brazil, which concluded in June, ... sorghum evaluation areas for part of the growing season. ...
Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... Madison Internet service provider SupraNet plans to ... the Aidmatrix Foundation, a global disaster-relief firm run by ... CEO Bryan Chan said in a press release that ... number of recent disasters the charity is responding to, ...
... of Wisconsin-Madison MBA program was ranked 51st in ... U.S. public university programs by the London Financial Times ... , ,These rankings are based on alumni careers and ... data provided by the schools and a survey of ...
... development assessment group for its high quality of life and ... States, released last week by the non-profit Corporation for Enterprise ... in business vitality and development capacity, based on 2004 data. ... subcategories that made up those three overall marks. , ,Perhaps ...
Cached Biology Technology:
(Date:7/11/2014)... 2014 Researchers have pioneered a revolutionary new ... technology, called Virtual Finger, allows scientists to move ... and synapses using the flat surface of their ... imaging studies orders of magnitude more efficient, saving ... across many areas of experimental biology. The software ...
(Date:7/11/2014)... hippocampus, the marginal division of the striatum is ... the impact degree of substance P in the ... Yan Yu and his team, College of Biophotonics, ... staining, that substance P receptor, neurokinin 1 was ... division of normal rats. Unilateral or bilateral injection ...
(Date:7/11/2014)... DUBLIN , July 11, 2014 ... the "International Biometrics Technology Market - Industry Analysis ... report to their offering. ... attacks and crimes has created a need for high ... methods used for personnel authentication such as username and ...
Breaking Biology News(10 mins):Virtual finger enables scientists to navigate and analyze complex 3D images 2International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2
... the Hebrew University of Jerusalem have achieved, for the ... cells of Fragile X patients. The discovery paves the ... gene expression in Fragile X patients. Fragile X ... retardation, affecting hundreds of thousands of patients worldwide. The ...
... differences between plants and animals, subtle ones lie concealed within ... animal cells, the nucleus contains DNA, which condenses into ... counted at that stage, revealing the chromosome number ... number spans a relatively short range across animal species (2-296: ...
... (Santa Barbara, Calif.) After an announcement by federal ... searching for a way to treat Alzheimer,s Disease are ... them to test a therapy for a genetically predestined ... incredible," said UC Santa Barbara neuroscientist Ken Kosik, who ...
Cached Biology News:Stem cell research paves way for progress on dealing with Fragile X retardation 2Does polyploidy play a role in the onset of the Italian endemic flora? 2Clinical trials for Alzheimer's disease preventative drug to begin early 2013 2Clinical trials for Alzheimer's disease preventative drug to begin early 2013 3
Smac/DIABLO (CT) - IgG for Western Blotting, Host: Rabbit Family: Smac/DIABLO...
Mouse Amphiregulin MAb (Clone 206220)...
Anti-Digoxigenin-AP, Fab fragments; from sheep...
The antibody recognizes the peptide sequence ESESQGPK (aa 441-448) of human class III beta-tubulin specific for neurones....
Biology Products: