Navigation Links
Quantitative Measurement of Cell Proliferation,,,Using the BrdU ELISA: A Comparison,,,Between Colorimetric and Chemiluminescent Detection

e examined the colorimetric and the chemiluminescence variants of the BrdU ELISA (Roche Molecular Biochemicals) in a series of parallel experiments.

Materials and Methods

Details of our special cell culture system and a short review on current measurement techniques for cell proliferation has been published [1-4]. We have investigated the proliferation of two distinct human prostatic carcinoma cell lines, P1/289 and P1/373 previously established in our laboratory.

In preliminary experiments, we had already defined the relevant cultivation and labeling intervals as well as the optimal cell concentrations for these cells. We have now investigated the influence of growth factors (epidermal growth factor, EGF) as well as the effects of insulin (Ins) and hydrocortisone (HC) on DNA synthesis in the cell lines P1/289 and P1/373. For this experiment, 104 cells were seeded in standard 96-well microtiter plates in a volume of 200 l medium/well and cultivated in complete medium (DMEM/F12, Life Technologies) containing 10 % fetal calf serum (FCS) supplemented with EGF (10 ng/ml), hydrocortisone (10 ng/ml), cholera toxin (CT, 10 ng/ml), and insulin (4 g/ml). To examine the effects of the additives (DMEM/F12+) on cell proliferation, the cell lines were also cultivated in parallel in their absence (DMEM/F12-).

A second application concerned another cell culture model: To prevent the outgrowth of fibroblasts when establishing cell cultures from heterogeneous tissue samples, cultivation of epithelial cells in the presence of cholera toxin is recommended. When added to normal cell culture medium, this toxin selectively suppresses the growth of fibroblasts. Accordingly, we cultured human fibroblasts obtained from explants (ZF 07) in microtiter plates. The rate o
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
5. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/25/2014)... , July 25, 2014  Galmed Pharmaceuticals Ltd. (Nasdaq: ... focused on the development and commercialization of a once-daily, ... cholesterol gallstones, today announced financial results for the period ... Financial Summary: , Reported cash and cash equivalents ... $137,000 at December 31, 2013.  , Reported a ...
(Date:7/25/2014)... July 25, 2014 Sinovac Biotech Ltd. (Nasdaq: ... China , today announced that it will ... June 30, 2014, after market close on Thursday, August 14, ... to the market opening on Friday, August 15, 2014, at ... China Standard Time) to review the Company,s financial results and ...
(Date:7/24/2014)... (PRWEB) July 24, 2014 Gain ... Global Supply Chain of the biotech industry. Nominations ... Innovation Awards, brought to you by the ... organization of supply chain management professionals for the ... to stakeholders of the Biotech industry – Manufacturers, ...
(Date:7/24/2014)... 2014  ECC West Africa, LLC (ECC) signed a ... University College Hospital (UCH) Ibadan Board of Management for ... design, construct, and operate and maintain a new, state-of-the-art ... Under the first of the 4-phase project, ECC will ... applications. UCH Ibadan intends to finance and develop the ...
Breaking Biology Technology:Galmed Pharmaceuticals Reports First Half 2014 Financial Results 2Galmed Pharmaceuticals Reports First Half 2014 Financial Results 3Galmed Pharmaceuticals Reports First Half 2014 Financial Results 4Galmed Pharmaceuticals Reports First Half 2014 Financial Results 5Galmed Pharmaceuticals Reports First Half 2014 Financial Results 6Sinovac to Host Conference Call to Report Second Quarter 2014 Unaudited Financial Results 2Call for Submission: BSMA’s Supply Chain Management Innovation Awards 2Call for Submission: BSMA’s Supply Chain Management Innovation Awards 3ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2
... June 27 the U.S. Supreme Court decided Metro-Goldwyn-Mayer ... decision affects how software companies and other technology providers ... be used to play, transmit or store copyrighted content. ... favor of MGM, reversing a lower court decision favoring ...
... MDVIP, Inc. , named former Wisconsin Governor and U.S. ... of the MDVIP Committee on Cost Reduction through Preventive Healthcare. ... of a network of 82 physicians in 14 states. Member ... the traditional primary care practice of 2,500 patients, and focus ...
... Wis. It should have been another normal London ... it was a day touched by terror. , ,Shortly after ... trader Matthew rode a borrowed bicycle from the citys Financial ... their two daughters, Madeline and Lillie, made it safely home. ...
Cached Biology Technology:Tech vendors could be liable for customer behavior - and not just file sharing 2Tech vendors could be liable for customer behavior - and not just file sharing 3Western way of life is under attack from jihad sub-culture 2Western way of life is under attack from jihad sub-culture 3
(Date:7/25/2014)... Stowers Institute for Medical Research have identified the ... pheromones crucial to triggering the mating process in mice. ... male mouse detect pheromones that indicate when a female ... know if the female mouse is ovulating and ready ... trigger mating. Stowers, researchers believe mice developed this system ...
(Date:7/24/2014)... NY (July 24, 2014) Scientists at The ... are one step closer to creating a viable ... patient,s own cells. , For the first ... cells lines from skin samples of patients with ... an accelerated protocol to induce these stem cells ...
(Date:7/24/2014)... Scientists at The New York Stem Cell ... to creating a viable cell replacement therapy for ... , For the first time, NYSCF scientists generated ... samples of patients with primary progressive multiple sclerosis ... induce these stem cells into becoming oligodendrocytes, the ...
Breaking Biology News(10 mins):It takes two to court 2It takes two to court 3NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2NYSCF scientists 1 step closer to cell therapy for multiple sclerosis patients 2
... Calif., Nov. 14, 2012   Masimo (NASDAQ: ... present at the 24th Annual Piper Jaffray Health Care Conference at ... 28, 2012, at 2:00 p.m. Eastern Time.  A live audiocast ... at www.masimo.com .  A replay of the audiocast will ...
... Conn. The University of Connecticut announced today that ... funded by the Bill & Melinda Gates Foundation. ... Engineering) and Daniel J. Gage (Molecular & Cell Biology) ... project intended to increase crop yields in developing countries ...
... Mich. A study led by a Michigan State University ... type of specialized foot massage practiced since the age of ... perform daily tasks. Funded by the National Cancer Institute ... Forum , it is the first large-scale, randomized study of ...
Cached Biology News:Masimo to Present at 24th Annual Piper Jaffray Health Care Conference 2Masimo to Present at 24th Annual Piper Jaffray Health Care Conference 3UConn receives Grand Challenges Explorations Grant for research in global health and development 2Ancient foot massage technique may ease cancer symptoms 2
... to significantly increase the shelf life of ... increased at room temperature storage conditions in ... is subjected to 0.2 micron filtration, and ... formulation insures consistently high levels of activity ...
... strength adhesive system which facilitates the easy removal ... either side of the frame are of differing ... has an easy release adhesive whereas the reverse, ... bond. This means that when the coverslip is ...
... filters suitable for use in the Microsart e.motion ... each filter, in a specially designed individual package ... of the band of membrane filter units ensures ... shape of the sealed band guarantees uniform dispensing ...
...
Biology Products: