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Quantitative Analysis of the Cell Cycle in Imaging Applications

s this assay to be multiplexed with other applications. For example, the Ki-67 stain could be substituted with a marker for pathway activation. Therefore this assay can work as a system of building blocks where users can choose their preferred dyes and antibodies in combination to generate higher dimensional data sets.

References

1. Endl, E., P. Steinbach, R. Knu chel and F. Hofstadter. 1997. Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/7AAD and immunolabeling technique. Cytometry . 29:233-241.

2. Goto, H., Tomono Y., Ajiro, K., et al.. 1999. Identification of a novel phosphorylation site on the histone H3 coupled with mitotic chromosome condensation . J. Biol. Chem 274, 25543-25549.

3. Kill, I.R. 1996. Localization of the Ki-67 antigen within the nucleolus. J. Cell Science. 109:1253-1263.

4. Miltenburger, H. G., G. Sachse and M. Schliermann. 1987. S-phase cell detection with a monoclonal antibody. Dev. Biol. Stand. 66:91-99.

5. Moran, R., Z. Darzynkiewicz, L. Staiano-Coico and M. R. Melamed. 1985. Detection of 5-bromodeoxyuridine (BrdUrd) incorporation by monoclonal antibodies: role of the DNA denaturation step. J. Histochem. Cytochem. 33:821-827.

6. Shapiro, H. M., Practical Flow Cytometry, 3rd Edition, Wiley-Liss, New York. Vanderlaan, M. and C. B. Thomas. 1985. Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6:501-505.

7. Starborg, M., K. Gell, E. Brundell, and C. Hg. 1996. The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J. Cell Science. 109:145-153.


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