Navigation Links
Quantitative Analysis of the Cell Cycle in Imaging Applications

The staining pattern for BrdU and phospho-Histone H3 found in control cells was consistent with a heterogeneous cell population ( Figure 1 ). We found that approximately 3% were in M phase based on the positive phospho-Histone 3 staining and approximately 22% were in S phase based on the BrdU results ( Figure 1D ). When cells were treated with either colcemid or aphidicolin changes in cell cycle status were seen ( Figure 1 and Figure 3 ). In colcemid treated cells (eg, 500 ng/mL) the percent cells in S phase remained similar (~20%) while the percent cells in M phase doubled to approximately 7% ( Figure 1D ). In aphidicolin treated cells (eg, 500ng/mL), the percent cells in S phase dropped to 0% while the percent cells in M phase dropped slightly to approximately 2%. Figure 2 shows the dose-response relationship of BrdU incorporation in response to increasing concentrations of colcemid and aphidicolin. BrdU indicates cells in S phase and colcemid had no significant effect at any concentration. Aphidicolin decreased the number of S-phase cells in a dose-dependent manner.

Discussion

To assure homogenous responses in cellular assays, cells are often synchronized using multiple rounds of cell-cycle arrest and release. This adds complexity and unreliability to the assay in question and is incompatible with many assays especially stem cell or primary cell assays. We therefore developed a versatile application that utilizes standard antibody treatment to identify the cell cycle phases and applied it to automated imaging. As a stand-alone application, the assay quantifies the degree to which cells are arrested in the different cycle phases after drug treatment making it useful in oncology and other fields where drug-responses are suspected to be dependent on the specific cell cycle phase. In addition, the nature of the exchangeable antibodies allow
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
8. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
9. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
10. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
11. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Quantitative Analysis the Cell Cycle Imaging Applications

(Date:9/16/2014)... According to Jeff Howell, Partner of Nidea ... new development in Downtown Toronto is expanding at a ... 755 storeys of new development last week (including three ... dominating the Toronto skyline for the foreseeable future in ... for new development. , As the Toronto city council ...
(Date:9/15/2014)... 16, 2014 Cancer Immunotherapy ... Adoptive T-cell Therapies is a new market research ... the three principal types of therapeutics that have ... in cancer immunotherapy (which is often called "immuno-oncology") ... and Adoptive cellular immunotherapy. , The regular ...
(Date:9/15/2014)... fast when a patient has sepsis, a life-threatening condition ... blood -- often too fast for antibiotics to help. ... developed by a team at Harvard,s Wyss Institute for ... treat sepsis. , "Even with the best current ... at least 30 percent of the time," said Mike ...
(Date:9/15/2014)... , Sept. 15, 2014 Zymo ... viral transport solution for safe ambient temperature storage ... molecular testing. Importantly, the infectivity of agents in such ... maintaining the integrity of the viral nucleic acids ... the recent Ebola epidemic in West ...
Breaking Biology Technology:ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 2Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 3Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 4Cancer Immunotherapy Market: Immune Checkpoint Inhibitors, Cancer Vaccines, and Adoptive T-cell Therapies 5Blood-cleansing biospleen device developed for sepsis therapy 2Blood-cleansing biospleen device developed for sepsis therapy 3Blood-cleansing biospleen device developed for sepsis therapy 4Safe storage and transport of Ebola, Mers-CoV, and other viruses: Groundbreaking viral inactivation solution capable of preserving nucleic acids 2Safe storage and transport of Ebola, Mers-CoV, and other viruses: Groundbreaking viral inactivation solution capable of preserving nucleic acids 3
... Dr. ... San Antonio and South Central Texas, , ... (PRWEB) June 15, 2010 -- For those looking for a trimmer physique without ... Zeltiq ™ Procedure, a new non-invasive body contouring procedure, at his practice - Advanced ...
... data from a retrospective cohort study showed that up to three quarters ... have a substantial adverse effect on major PD-related clinical and health economic ... Disease and Movement Disorders in Buenos Aires, Argentina ( June ... , "The ...
... leading publication on diversity and business, has recognized University Hospitals as a leader among ... , , ... Companies for Diversity based on results from an extensive questionnaire. This is the first ... , , ...
Cached Biology Technology:Dr. Suresh Koneru Introduces Zeltiq Non-Invasive Procedure For Fat Reduction 2Dr. Suresh Koneru Introduces Zeltiq Non-Invasive Procedure For Fat Reduction 3New Study Showed the Impact of Gastrointestinal Disorders in Patients With Parkinson's Disease 2New Study Showed the Impact of Gastrointestinal Disorders in Patients With Parkinson's Disease 3New Study Showed the Impact of Gastrointestinal Disorders in Patients With Parkinson's Disease 4Diversity Publication Names University Hospitals One of Top Hospital Systems in the Nation 2
(Date:9/15/2014)... 2014) The so-called central dogma of molecular ... provided a simplified explanation for how genetic information ... , In reality, of course, the process is ... nearly 60 years ago by Nobel Laureate Francis ... one, there are multiple types of RNA, three ...
(Date:9/15/2014)... a simple, mechanical solution to avoid chromosome sorting ... Science Express. , This natural ... lead to infertility, miscarriage, or congenital conditions. ... problems, but what exactly goes wrong is often ... Trust Center for Cell Biology at the University ...
(Date:9/15/2014)... that a single group of microorganisms may be responsible ... the oceans, with implications for the global carbon cycle ... essential molecule required by most life on this planet, ... of microorganisms because it is so large and complex. ... maintaining the brain and nervous systems, as well as ...
Breaking Biology News(10 mins):Scientists discover RNA modifications in some unexpected places 2Cells simply avoid chromosome confusion 2Cells simply avoid chromosome confusion 3Researchers discover new producer of crucial vitamin 2
... University of North Carolina at Chapel Hill has received ... & Melinda Gates Foundation. The grant will support ... Tsuruta, PhD, and Paul Dayton, PhD, titled "Ultrasound as ... professor in the Laboratories for Reproductive Biology in UNC,s ...
... It has been a hard winter for Monarch butterflies, according ... of Kansas. Taylor said that low temperatures, storms and habitat ... "I spend a lot of time fretting over ... for factors or data that will help me understand the ...
... team of researchers led by University of Notre Dame ... thinking about the genomics of speciation. Titled "Widespread genomic ... edition of the Proceedings of the National Academy ... how the genomes of newly forming species should differ ...
Cached Biology News:UNC researchers receive $100,000 Grand Challenges Exploration Grant to develop male contraceptive 2As monarch butterflies journey north, gardeners can help protect species, researcher says 2Paper offers new insights into the genomics of speciation 2
Spastin (Sp 3G11/1)...
mCD-1 10 transcription reactions...
... Rabbits x 2 , ... bleed x1 /each , Immunizations/Boost injections ... (app. 5 ml) x1 /each , ... /each , Final Bleed ~ 100 ml blood ...
Mouse VCAM-1/CD106 MAb (Clone 112702)...
Biology Products: