Navigation Links
Quantitative Analysis of the Cell Cycle in Imaging Applications

ination to specifically inform on the M and S phases of the cell cycle while it is still possible to drop in an additional specificity of interest to the user. For this assay, Hoechst 33342 was used to identify all nuclei and an anti-phospho-Histone 3 antibody was used to identify cells in M phase.1 Similarly immunofluorescent staining of incorporated bromodeoxyuridine (BrdU) is used to determine the frequency of cells that had synthesized DNA.2, 3, 4, 5 In this method, BrdU (an analog of the DNA precursor thymidine) is incorporated into newly synthesized DNA by cells entering into and progressing through the S phase of the cell cycle. The drop in choice for this assay was a fluorescently labeled anti-Ki-67 antibody. Ki-67 is present in all cycling cells but is not present in quiescent cells (G0). The Ki-67 staining pattern is variable depending on the phase of the cycle, in the G1/S/G2 phases it is punctuate, whereas in late G2/M it appears throughout the entire nucleus (see Table 1 ).6,7

The BD Pathway Bioimager is well suited for the development of complex assays due to its wide range of acquisition and analysis tools. Using a broad-spectrum illumination source, the system enables the user to select the antibodies based on their compatibility with the assay rather than with specific laser wavelengths. The 16 excitation filter choices of the BD Pathway Bioimager allow the setup of several multi-color assays with minimal bleed-through which enables the system to be used in a wide variety of demanding applications.

Methods

HeLa cells (a human cervical cancer cell line, ATCC, #CCL-2) were seeded in 96-well clear bottom tissue culture plates optimized for automated imaging applications (Cat. No. 35-3219) at 15,000 cells/well in DMEM (Invitrogen, 11965-084) complete media. Eighteen hours later cells were treated with one of two different metabolic inhibitors, aphidicolin (Sigma-Aldric
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
8. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
9. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
10. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
11. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Quantitative Analysis the Cell Cycle Imaging Applications

(Date:7/22/2014)... , July 22, 2014   BioTE Medical ... using natural, bio-identical hormone pellets, today announced the ... to the company.   Dr. Rouzier is joining the ...     Dr. Rouzier was residency trained ... and is a board certified emergency physician still ...
(Date:7/22/2014)... Chicago, IL (PRWEB) July 22, 2014 ... will a clear and concise visual representation of history, ... Mr. Novak was always regaled by family history stories ... historic location as the "old country." Pictures and 8mm ... and undiscovered side of the family, and Steve ordered ...
(Date:7/22/2014)... SHENZHEN , Cina, 22 luglio 2014 /PRNewswire/ ... filiale di BGI, la maggiore organizzazione di genomica del ... servizio di sequenziamento dell,intero esoma umano con base la ... Complete Genomics, molto apprezzata nel settore per essere ... il 99.999% di precisione e una rilevazione altamente sensibile ...
(Date:7/22/2014)... , July 22, 2014  Semler Scientific, ... emerging medical risk assessment company that develops patented ... and evaluating chronic diseases, today announced that it ... the open of U.S. financial markets on July 25, ... will host a conference call at 11 a.m. EDT, July ...
Breaking Biology Technology:Dr. Neal Rouzier Joins BioTE Medical 2Dr. Neal Rouzier Joins BioTE Medical 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4BGI Tech lancia un servizio di sequenziamento dell'intero esoma umano sulla piattaforma avanzata di Complete Genomics 2Semler to Report Second Quarter 2014 Financial Results and Host Conference Call on July 25, 2014 2
... TomoTherapy, Inc . has sold three of its advanced ... centers, the company announced. , ,The H. Lee ... University of South Florida in Tampa, will install three of ... by August of this year. The purchase price was not ...
... their personal information stolen. What's worse is that many ... of birth, social security number, or credit card information ... proper steps to protect themselves, their businesses, or their ... credit card or bank account information, can steal thousands ...
... - The Wisconsin Legislature has passed a bill that ... to commercialize their discoveries, but two other bills endorsed ... the remainder of the 2005-07 legislative session. , ,On ... Senate enacted their respective versions of Senate Bill 338, ...
Cached Biology Technology:Leading cancer institute to install three TomoTherapy systems 2Cracking down on identity theft 2Legislature adopts one tech bill, leaves two others twisting 2Legislature adopts one tech bill, leaves two others twisting 3Legislature adopts one tech bill, leaves two others twisting 4
(Date:7/22/2014)... not be good for the average steak, but it ... prepare to survive Alaska,s winter cold. , "Alaska wood ... a steak does in your freezer and the frog ... shape than the steak," said Don Larson, University of ... recent paper demonstrating that freeze tolerance in Alaska wood ...
(Date:7/22/2014)... for Health Policy and Economics have linked tighter ... rates for schizophrenic individuals. , The study comes ... health actually save money, when other costs are ... an extra approval step before tests or treatments ... called prior authorization is intended to encourage ...
(Date:7/22/2014)... Highly purified crystals that split light with uncanny ... optics and, potentially, computers that manipulate light instead ... techniques, such as etching them with a precise ... expensive. , Now, researchers at Princeton and Columbia ... allow scientists to customize and grow these specialized ...
Breaking Biology News(10 mins):Alaska frogs reach record lows in extreme temperature survival 2Alaska frogs reach record lows in extreme temperature survival 3Are state Medicaid policies sentencing people with mental illnesses to prison? 2Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... invertebrates to a wide range of temperature and ... species could have colonised the ocean depths. ... various times during Earth,s history caused extinctions of ... (>4,000 m) depths. These extinctions were apparently followed ...
... This release is available in Spanish . , ... distributions of 100 crops; 640 species of crop diseases, pests, ... and neighboring countries. Downloadable maps and geographic information system (GIS) ... that relating major wheat production areas to concentrations of Russian ...
... new study led by a University of Florida researcher uses ... evidence some of the fish swim directly to targeted locations. ... to orient at large distances, with tiger sharks swimming in ... resource areas about 30 miles away, said lead author Yannis ...
Cached Biology News:Shallow-water shrimp tolerates deep-sea conditions 2USDA and Russian scientists develop high-tech crop map 2New UF study shows some sharks follow 'mental map' to navigate seas 2New UF study shows some sharks follow 'mental map' to navigate seas 3
... White solid.. PACKAGED UNDER INERT GAS. ... reaction is "driven" to completion. A protective agent ... found in many other preparations. Blocks the lethal ... by titration. Contaminants: Heavy metals: ≤1 ...
... most ``special`` dye stains are $6.00 ... other non-routine) stains are $9.00. ... paraffin block, staining, coverslipping and labeling ... senile plaques) ,Bodian`s Stain ,Copper Stain ...
... The S. pombe Whole Genome ... location analysis of yeast (S. pombe) ... immunoprecipitation (ChIP) with Agilent DNA microarrays. ... reliable binding data with greater true ...
... effort to help promote genomics-based research with S. ... set that contains PCR primers to amplify all ... In addition, primer pairs are included to amplify ... and N315 strains of S. aureus. The ORFmer ...
Biology Products: