Navigation Links
Quantitative Analysis of the Cell Cycle in Imaging Applications

Introduction

Quantitative analysis of stages in the cell cycle is commonly used in cell biology since many pharmacological interventions aim to modulate the cell cycle in some way. We present here an application that can be used in adherent cells and optionally multiplexed for the use in automated cell signaling analysis and drug-screening applications.

The cell cycle consists of a series of processes involved in the growth, replication, and division of cells and can be divided into two major stages, interphase (the phase between mitotic events) and mitosis. There are three distinct, successive stages within interphase, namely G1, S, and G2 phases. During G1 (first gap), cells monitor their environment and grow (synthesize RNA and proteins). Once conditions are optimal, cells commit to DNA synthesis (S phase) and replicate their chromosomal DNA. The G2 phase (second gap) follows when cells continue to grow and prepare for mitosis (M phase, division). The G2 gap allows time for the cell to complete its DNA replication before initiating mitosis.

During mitosis, the cell cycle is completed giving rise to two daughter cells, each of which contains the same genetic material as the original cell and approximately half of its G2 level of cytoplasm. In addition to these specific stages, a G0 phase has been described for cells that exit from the cellcycle and enter a quiescent, non-dividing state. In response to external stimuli, cells may undergo reactivation and leave G0 and enter the G1 phase of the cell-cycle. Another consequence of cellular activation may be the induction of programmed cell death (apoptosis).

Understanding the specific phase of a cell is important when cells display heterogeneous responses to drugs as is often the case in high-content applications.

This four color application demonstrates how fluorescent antibodies can be used in comb
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
8. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
9. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
10. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
11. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Quantitative Analysis the Cell Cycle Imaging Applications

(Date:1/15/2014)... 15, 2014 More than 5 million ... 1 in 3 seniors will die with Alzheimer’s or ... jaw-dropping figures have shocked many Americans into looking for ... prevent these tragic age-related cognitive disorders. Jonathan Weisman, president ...
(Date:1/15/2014)... 2013 was a banner year of ... They saw continued independent research led by the team ... a $1 million grant from the Susanne Marcus Collins ... a peer reviewed journal, Amy Grant highlighted Brainwave Optimization® ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, April ... Location: Warrington Country Club, 1360 Almshouse Road, Warrington, Pa. ... nonprofit organization solely dedicated to finding a cure for hepatitis ... worldwide, will host its annual Crystal Ball on Friday, April ...
(Date:1/14/2014)... Jan. 14, 2014 As pet owners drew up their lists ... them wrote: "Take better care of my furry companion." Nowadays there ... and top-brand carrying cases to take the little canine or feline ... feel compelled to buy some pricey toys at the pet store. ...
Breaking Biology Technology:Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... PBI-1402 did not require blood ... ... Inc.,(TSX: PLI) ("ProMetic") announces that oral PBI-1402 demonstrates,significant activity in patients ... ("RBC") transfusion., Only 2 patients out of 28 (7%) treated ...
... Contributes to First Quarter Performance; Company Taking ... Actions to Address New Challenges, ... ) today reported financial results for the first,quarter of 2008, ... (acquired in November 2007) and addressed recent,events affecting the Merck/Schering-Plough ...
... types of image are better than one for analyzing tumors ... method to determine the amount of oxygen in a tumor very ... linked to a poor outcome. A new method to do this ... time has been developed and used to image tumors in mice ...
Cached Biology Technology:Oral PBI-1402 Demonstrates Significant Activity in Chemotherapy-Induced Anemia Phase II Clinical Trial 2Oral PBI-1402 Demonstrates Significant Activity in Chemotherapy-Induced Anemia Phase II Clinical Trial 3Schering-Plough Reports Financial Results for First Quarter of 2008 2Schering-Plough Reports Financial Results for First Quarter of 2008 3Schering-Plough Reports Financial Results for First Quarter of 2008 4Schering-Plough Reports Financial Results for First Quarter of 2008 5Schering-Plough Reports Financial Results for First Quarter of 2008 6Schering-Plough Reports Financial Results for First Quarter of 2008 7Schering-Plough Reports Financial Results for First Quarter of 2008 8Schering-Plough Reports Financial Results for First Quarter of 2008 9Schering-Plough Reports Financial Results for First Quarter of 2008 10Schering-Plough Reports Financial Results for First Quarter of 2008 11Schering-Plough Reports Financial Results for First Quarter of 2008 12Schering-Plough Reports Financial Results for First Quarter of 2008 13Schering-Plough Reports Financial Results for First Quarter of 2008 14Schering-Plough Reports Financial Results for First Quarter of 2008 15Schering-Plough Reports Financial Results for First Quarter of 2008 16Schering-Plough Reports Financial Results for First Quarter of 2008 17Schering-Plough Reports Financial Results for First Quarter of 2008 18Schering-Plough Reports Financial Results for First Quarter of 2008 19JCI table of contents: April 22, 2008 2JCI table of contents: April 22, 2008 3JCI table of contents: April 22, 2008 4JCI table of contents: April 22, 2008 5JCI table of contents: April 22, 2008 6JCI table of contents: April 22, 2008 7JCI table of contents: April 22, 2008 8JCI table of contents: April 22, 2008 9JCI table of contents: April 22, 2008 10JCI table of contents: April 22, 2008 11JCI table of contents: April 22, 2008 12
(Date:4/17/2014)... MANHATTAN, KAN. A Kansas State University engineer ... security and remotely detects improvised explosive devices. The ... , William Dunn, the Steven M. and Kay ... of mechanical and nuclear engineering, and his research ... explosives hidden underground or in car trunks. The ...
(Date:4/17/2014)... news release is available in German . ... to pay the protection money demanded of him, he can ... seldom required, however, as fear of the consequences is enough ... observed in parasitic birds, which lay their eggs in other ... out, the brood parasites take their revenge by destroying the ...
(Date:4/17/2014)... many serious conditions such as heart disease, epilepsy and ... therapies. Unfortunately, there is still much scientists do not ... and unexpected insight into the structure of sodium channels ... - which are responsible for ,fine-tuning, the activity of ... recent edition of the Journal of Biological Chemistry ...
Breaking Biology News(10 mins):Patented research remotely detects nitrogen-rich explosives 2Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3Structure of sodium channels different than previously believed 2
... in the May 2008 print issue of The FASEB ... dystrophy are missing a protein called c-FLIP, which the ... By targeting the cellular and molecular mechanisms responsible for ... stop muscle wasting from limb-girdle muscular dystrophy and other ...
... screening method for analyzing interactions between cells and ... more than half, researchers from the National Institute ... report in the new issue of Advanced Materials.* ... methods, enables rapid assessment of the biocompatibility and ...
... (WHOI) and the Bioacoustics Research Program (BRP) at the ... international energy company and federal regulators to listen for ... New England waters. Building on advances in ocean ... built and installed ten auto-detection buoys to listen for ...
Cached Biology News:Researchers discover molecular basis of a form of muscular dystrophy 2New 3-D test method for biomaterials 'flat out' faster 2New whale detection buoys will help ships take the right way through marine habitat 2New whale detection buoys will help ships take the right way through marine habitat 3
Fast, sensitive quantitation of Mouse IgM concentration....
... vial contains 200 g of lyophilized peptide ... (C-terminal amino acids 380-402; GLTPSAWEASSLRSSRHSGLSHF. ... for production of Cayman's EP1 receptor polyclonal ... used in conjunction with this antibody to ...
... x 25 mm; approximately 1 mm thick, ... sections, sandblasted frosted writing ... markings will not rub off or wash off ... store at room temperature ...
Complete cell culture media with cytokines...
Biology Products: