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Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

promoters studied.

In conclusion, our studies introduce a powerful technique (ChIP assay followed by real-time PCR) for quantitative examination of the association of proteins with DNA in vivo. Our results demonstrate that protein-DNA associations in vivo are governed not only by the intrinsic affinity of specific proteins for DNA, but also by the structure of chromatin, which may physically restrict accessibility to some DNA sequences.


References
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Ohneda K et al., The homeodomain of PDX-1 mediates multiple proteinprotein interactions in the formation of a transcriptional activation complex on the insulin promoter, Mol Cell Biol 20, 900911 (2000)

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