Navigation Links
Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

Swarup K Chakrabarti and Raghavendra G Mirmira*, Departments of Medicine and Pharmacology, Division of Endocrinology and Metabolism, University of Virginia Health Sciences Center, 450 Ray C Hunt Dr, Box 801407, Charlottesville, VA 22903 USA

*Corresponding author


Introduction
The chromatin immunoprecipitation (ChIP) assay has been used in recent years to identify the association of histone complexes and transcription factors with specific genomic DNA targets in cells of Drosophila or yeast. As shown in Figure 1, the technique first involves the treatment of cells with formaldehyde or ultraviolet light to covalently crosslink proteins to DNA. The chromatin from the cells is then isolated and fragmented (by restriction digestion or sonication), and the protein of interest is immunoprecipitated using a specific antibody. The coimmunoprecipitated DNA fragments are then liberated from the protein by treatment with proteinase K, and the DNA fragments are identified by Southern blotting or PCR (Kuo and Allis 1999). More recently, studies have established the utility of the ChIP assay in studying histone complex associations and transcription factor binding in mammalian cells as well (Boyd and Farnham 1999). However, a potential barrier to the quantitative study of some mammalian transcription factors by the ChIP assay is their relatively low level of expression in cells, coupled with the rather large size of the mammalian genome compared to yeast or Drosophila. Southern blot detection of DNA does not offer the necessary sensitivity for such studies, nor is traditional PCR easily amenable to quantitation. However, we demonstrate here that real-time PCR wit
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:5/21/2015)... CA (PRWEB) May 21, 2015 ... has announced a partnership with PicnicHealth, a healthcare ... patients. Participants diagnosed with Inflammatory Bowel Disease (IBD) ... as a complementary uBiome research kit. Both companies ... , For more information on ...
(Date:5/21/2015)... May 21, 2015 Patent Offering, The ... for diagnostic or therapeutic imaging within a body lumen ... an endoscope having a low cost, single-use disposable illumination ... date was October 18, 2013 and the patent approval ... enables the physician to customize the lighting and magnification ...
(Date:5/21/2015)... - RepliCel Life Sciences Inc. (TSX.V: RP) (OTCQB: ... on the development of autologous cell therapies, announced ... Society for Cellular Therapy (ISCT) on RepliCel,s autologous ... a Phase 1/2 clinical trial.  The presentation, taking ... PM to 7:00 PM local time, will review ...
(Date:5/20/2015)... DUBLIN , May 20, 2015 Research and ... addition of the "Top Technologies in HW_Technical Insights" ... division of this report evaluated technology trends in the ... trends that are likely to have an impact in ... depth analysis of the top 10 health and wellness ...
Breaking Biology Technology:uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 2IpAuctions™ Presents The Worlds Smallest Disposable Illuminated Endoscope For Auction 3RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 2RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 3Top Technologies in the Health and Wellness Industry 2015 2
... is part of Aeterna Zentaris, strategy of personalized approach to ... with doxorubicin, being investigated in multiple cancers , ... QUÉBEC CITY, ... Nasdaq: AEZS ), (the "Company"), a late-stage drug development ...
... ... enhance and treat cognition grew 31% in 2009, to $295M worldwide; and is forecasted to ... ... revenues for digital technologies to assess, enhance and treat cognition, or digital brain health ...
... ... of the Art 3D Dedicated Neurology Suite, , ... (PRWEB) June 28, 2010 -- TrueVision 3D Surgical announced ... Arizona to adopt TrueVision® 3D Surgical Visualization, where multiple 3D flat panel displays and ...
Cached Biology Technology:Aeterna Zentaris Announces Collaboration with Almac to Develop Therapy and Companion Diagnostic in Cancer 2Aeterna Zentaris Announces Collaboration with Almac to Develop Therapy and Companion Diagnostic in Cancer 3Aeterna Zentaris Announces Collaboration with Almac to Develop Therapy and Companion Diagnostic in Cancer 4New Report Finds A Brain Health Revolution in the Making, Driven by Digital Technology and Neuroplasticity Research 2New Report Finds A Brain Health Revolution in the Making, Driven by Digital Technology and Neuroplasticity Research 3New Report Finds A Brain Health Revolution in the Making, Driven by Digital Technology and Neuroplasticity Research 4New Report Finds A Brain Health Revolution in the Making, Driven by Digital Technology and Neuroplasticity Research 5Cardon Children's Medical Center Adopts TrueVision 3D Surgical Visualization Platform for Department of Pediatric Neurosurgery 2
(Date:4/21/2015)... , April 21, 2015 ... evolving government policies are boosting access control systems market ... According to a recently published report by TechSci ... 2020 ", the access control systems market in ... USD1.2 billion by 2020.The access control systems market in ...
(Date:4/17/2015)... 17, 2015 Increasing ... technological advancement to drive biometric systems market in ...   According to a recently released ... &  Opportunities, 2020 ", biometric systems market in ... growth at CAGR of over 22% through 2020. ...
(Date:4/13/2015)... NEW YORK , April 13, 2015 /PRNewswire/ ... study by TechSci Research, "Global Biometrics Market Forecast & ... to register a CAGR of around 14% till ... various governments for large scale implementation and review ... technology and launch of new products with greater ...
Breaking Biology News(10 mins):Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... itself as a nation that stretches from coast to coast ... the face of climate change? Yves Glinas, associate professor in ... in a surprising element: iron. In a study published ... candidate Karine Lalonde and graduate Alexandre Ouellet, as well as ...
... NEW YORK, March 14, 2012 A new research ... American Health at NYU Langone Medical Center shows the Bangladeshi ... care because of limited English proficiency and lack of diabetes ... the American Journal of Public Health , also found ...
... CORAL GABLES, FL (March 14, 2012)--Convincing speeches are central to ... we select our leaders? A newly-published paper in Proceedings ... men and women vote for male and female candidates with ... found to be perceived as more competent, stronger, and more ...
Cached Biology News:Fielding questions about climate change 2New study shines light on barriers to diabetes care in NYC Bangladeshi community 2
Peptide-affinity Purified Polyclonal Antibody to MAML1 / Mastermind Peptide with sequence VLPTCPMAEFALPR, from N Terminus of the protein sequence according to NP_055572....
DNA sequencing - plasmids or PCR fragments, 3-7 working days service....
LEAF™ Purified anti-human/mouse/rat ICOS...
Purified anti-human CD182 (CXCR2)...
Biology Products: