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Quantitation of the Protein MIA as a Marker for,,,Chondrocytes in Research Samples

(Roche Applied Science), the manufacturers instructions were precisely followed and the internal standard reagents provided were used.

Results and Application

MIA as a potential marker for chondrocyte differentiation and chondrocytic disorders

High levels of MIA expression were observed in differentiated chondrocytes. As a result, MIA was also investigated as a potential marker for cartilage tissue engineering and cartilage damage.

In cell culture of chondrocytes and tissue engineering of cartilage cell differentiation must be carefully monitored. The detection of collagen type II as a marker for chondrocytic differentiation is commonly used. However, using this marker does create problems as ELISAs are not feasible, and for RNA analysis, the cells are lost during extraction procedures. Therefore we established MIA as a chondrocytic marker.
MIA is a secreted molecule, making it easy to measure its levels in the cell culture supernatant. Detection by the MIA-ELISA is highly specific, sensitive, and quantitative. In cell-culture systems using human chondrocytes (Figure 1) and human mesenchymal stem cells (Figure 2), we were able to monitor the process of cell differentiation.

MIA serum concentrations in individuals with different rheumatic diseases were measured, correlated with inflammatory parameters and/or with the degree of joint destruction, and compared with healthy individuals and subjects suffering from melanoma [6]. Increased MIA serum concentrations were only found in individuals with rheumatic diseases associated with joint destruction, such as rheumatoid arthritis, osteoarthritis, HLA B27-associated oligoarthritis, and psoriatic arthritis. Within these rheum
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