(Roche Applied Science), the manufacturers instructions
were precisely followed and the internal standard
reagents provided were used.
Results and Application
MIA as a potential marker for chondrocyte
differentiation and chondrocytic disorders
High levels of MIA expression were observed in differentiated
chondrocytes. As a result, MIA was also investigated as a potential marker for cartilage tissue engineering
and cartilage damage.
In cell culture of chondrocytes and tissue engineering
of cartilage cell differentiation must be carefully monitored.
The detection of collagen type II as a marker for
chondrocytic differentiation is commonly used.
However, using this marker does create problems as
ELISAs are not feasible, and for RNA analysis, the cells
are lost during extraction procedures. Therefore we
established MIA as a chondrocytic marker.
MIA is a secreted molecule, making it easy to measure its
levels in the cell culture supernatant. Detection by the
MIA-ELISA is highly specific, sensitive, and quantitative.
In cell-culture systems using human chondrocytes
(Figure 1) and human mesenchymal stem cells (Figure 2),
we were able to monitor the process of cell differentiation.
MIA serum concentrations in individuals with different
rheumatic diseases were measured, correlated with
inflammatory parameters and/or with the degree of joint
destruction, and compared with healthy individuals and
subjects suffering from melanoma . Increased MIA
serum concentrations were only found in individuals
with rheumatic diseases associated with joint destruction,
such as rheumatoid arthritis, osteoarthritis, HLA
B27-associated oligoarthritis, and psoriatic arthritis.
Within these rheum
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