Navigation Links
Quantitation of the Protein MIA as a Marker for,,,Chondrocytes in Research Samples

MIA (Melanoma Inhibitory Activity) was cloned as a secreted protein from human melanoma cell lines [1]. MIA is expressed in and secreted from malignant melanomas and chondrocytes [2, 3]. As MIA expression in chondrocytes is dependent on the differentiation status of the cells it was also designated CD-RAP (cartilage-derived retinoic acid sensitive protein). Retinoic acid is known as a regulator of chondrocytic cell differentiation in vitro.

Recent results indicate an important role in tumor progression and metastasis as MIA mediates detachment of melanoma cells from extracellular matrix molecules such as fibronectin [4]. MIA expression levels closely parallel the capability of melanoma cells to form metastases in syngeneic animals. Recently, MIA-deficient mice were shown to have structural abnormalities in cartilage [5].

Materials and Methods

Cell culture

Human primary chondrocytes derived from articular cartilage were cultivated in DMEM supplemented with penicillin (100 U/ml), streptomycin (10 g/ml) (both Sigma), and 10 % fetal calf serum (Gibco) under a humidified atmosphere of 5% CO2 at 37 C, then split 1:2 at 80 % confluency. As chondrocytes spontaneously dedifferentiate in cell culture, redifferentiation of the cells was induced by treatment with transforming growth factor-b (TGF-b) for 4 days. Differentiation was controlled by determining collagen type II with RT-PCR. Human mesenchymal stem cells were obtained from BioWhittaker Europe (Poietics) and cultured as described by the manufacturer.


MIA was measured by a commercially available ELISA (Roche Applied Science), the manufacturers instructions were precisely followed and the internal standard reagents provided were used.

Results and Application

MIA as a potential marker for chondrocyte differentiation and chondrocytic disorders

High levels of MIA expression were observed in differentiated chondrocytes. As a result, MIA was also investigated as a potential marker for cartilage tissue engineering and cartilage damage.

In cell culture of chondrocytes and tissue engineering of cartilage cell differentiation must be carefully monitored. The detection of collagen type II as a marker for chondrocytic differentiation is commonly used. However, using this marker does create problems as ELISAs are not feasible, and for RNA analysis, the cells are lost during extraction procedures. Therefore we established MIA as a chondrocytic marker.
MIA is a secreted molecule, making it easy to measure its levels in the cell culture supernatant. Detection by the MIA-ELISA is highly specific, sensitive, and quantitative. In cell-culture systems using human chondrocytes (Figure 1) and human mesenchymal stem cells (Figure 2), we were able to monitor the process of cell differentiation.

MIA serum concentrations in individuals with different rheumatic diseases were measured, correlated with inflammatory parameters and/or with the degree of joint destruction, and compared with healthy individuals and subjects suffering from melanoma [6]. Increased MIA serum concentrations were only found in individuals with rheumatic diseases associated with joint destruction, such as rheumatoid arthritis, osteoarthritis, HLA B27-associated oligoarthritis, and psoriatic arthritis. Within these rheum atic diseases, the most significant increase in MIA serum concentrations was measured in subjects with rheumatoid arthritis, associated with rheumatoid factor positivity and joint destruction. Therefore, MIA may be useful to discriminate rheumatoid arthritis from nondestructive rheumatic diseases. Further studies are necessary to confirm these preliminary findings.

Our studies indicate that MIA may be a useful marker for cartilage diseases and the monitoring of chondrocytic differentiation in cell culture. We conclude that MIA has the potential to replace other markers due to its improved applicability.



Page: All 1 2 3

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Comparison of Different Methods of mRNA Quantitation
4. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
5. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
6. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
7. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
8. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
9. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
10. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
11. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
Post Your Comments:

(Date:11/23/2015)... ... November 23, 2015 , ... Shimadzu Corporation announces that it ... Unified Chromatography system. The award from R&D magazine recognizes Shimadzu’s Nexera UC system ... in the analytical and testing category. R&D Magazine chose the Nexera UC system ...
(Date:11/23/2015)... CAMBRIDGE, England and PISCATAWAY, ... -- Cambridge Crystallographic Data Centre ... of the Cambridge Structural Database (CSD) and the ... suites for researchers worldwide: CSD-Discovery to support the ... of crystalline materials, and CSD-Enterprise, the complete set ...
(Date:11/23/2015)... WASHINGTON , Nov. 23, 2015 ... research firm to exclusively serve the ... of its global strategic report, "Mesenchymal ... Published November 2015, it presents 250 ... mesenchymal stem cell (MSC) industry. ...
(Date:11/23/2015)... 2015 Research and Markets ( ) has ... Service Type, Product/Technology, Application and Geography - Global forecast to ... --> --> The overall service laboratory market ... to USD 5.45 Billion by 2020, at a CAGR of ... --> The market in APAC is expected ...
Breaking Biology Technology:
(Date:11/18/2015)... 2015 --> ... market report titled  Gesture Recognition Market - Global Industry ... 2021. According to the report, the global gesture recognition market was valued ... reach US$29.1 bn by 2021, at a CAGR of ... America dominated the global gesture recognition market ...
(Date:11/16/2015)... , Nov 16, 2015  Synaptics Inc. ... human interface solutions, today announced expansion of its ... ™ touch controller and display driver integration ... of smartphones. These new TDDI products add to ... (HD resolution), TD4302 (WQHD resolution), and TD4322 (FHD ...
(Date:11/10/2015)... 2015  In this report, the biomarkers ... product, type, application, disease indication, and geography. ... are consumables, services, software. The type segments ... efficacy biomarkers, and validation biomarkers. The applications ... development, drug discovery and development, personalized medicine, ...
Breaking Biology News(10 mins):