Navigation Links
Quantitation of a Dinitro Flavonoid (DNF) in Rat Plasma Using the Varian 1200L LC/MS/MS System

D. Garteiz, R. Morrow, and B. Alexander, TexMS Analytical Services, Houston, TX C.
Schmidt, Varian, Inc., Walnut Creek, CA

Introduction

Flavonoids are compounds that make up the pigments in fruits, vegetables, and herbs. There have been reports of flavonoids having multiple beneficial therapeutic effects for a large number of serious illnesses, such as rheumatoid arthritis, cancer, cataracts, anxiety, and depression. The objective of this work was to carry out a pharmacokinetic evaluation to determine a profile with the formulation being tested at increasing doses where there is pharmacological effect.

A Varian 1200L LC/MS/MS system was used for the quantitation for plasma levels of a novel dinitro-substituted flavone (DNF) in samples from a pharmacokinetic study following oral administration of the drug. Flavone was used as the internal standard. Structures are shown in Figure 1.

Instrumentation

Varian 1200L LC/MS/MS equipped with ESI source

Materials and Reagents

DNF provided by Faber-Kramer Pharmaceuticals, Houston, Texas.

Flavone (Catalog Number F602) from Sigma-Aldrich.

All other chemicals are reagent grade or HPLC grade.

Sample Preparation

A 200 μL aliquot of rat plasma containing DNF is spiked with 10 μL of flavone (0.2 ng) as the internal standard (ISTD). The rat plasma is made basic and extracted with hexane: chloroform (2:1, v/v).

After separation of the phases by centrifugation, the organic layer is transferred by pouring into a silanized conical tube and evaporated to dryness. The dry residue is reconstituted in 50 μL of methanol:water (60:40, v/v).

A 10 μL aliquot is injected into an ESI-LC/MS/MS for analysis using Selected Reaction Monito
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:1/14/2014)... Jan. 14, 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), ... commercializing innovative therapies addressing major unmet medical needs ... the Notice of Allowance from the United States ... RNAi compounds (sd-rxRNA®), for the treatment of fibrosis. ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
(Date:1/14/2014)... 2014 Kerr Corporation, a leading manufacturer of ... how-to information about dual arch impressions on its dental blog. ... Impressions,” the blog entry serves up a list of tips ... step-by-step demonstration by Dr. David Little as he crafts a ...
(Date:1/14/2014)... 14, 2014 Independent publisher Bitingduck Press, ... Montreal, Canada, has big ideas for 2014 in production ... goals are to produce high-quality, low-cost, DRM-free ebooks and ... businesses. , The first major development will be ...
Breaking Biology Technology:RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2Bitingduck Press Looks Forward to 2014 With Multimedia E-books 2
... (Nasdaq: ARAY ), the premier radiation oncology company, ... the Wedbush Life Sciences: Management Access Conference in New York ... PT. A live webcast of the presentation will ... Company,s Web site at http://www.accuray.com . The webcast replay ...
... Reportlinker.com announces that a new ... its catalogue: Global ... and Sales Forecasts by Country ... This report provides a worldwide ...
... Aug. 9, 2011 Bharat Renewable Energy Ltd ... largest petroleum company, has initiated a program with ... hybrids of Jatropha for the production of biodiesel in ... crop development effort to produce high performing hybrid varieties ...
Cached Biology Technology:Global Microbiology Testing Market: Facilities, Test Volumes, and Sales Forecasts by Country 2Bharat Renewable Energy Forms Alliance With SG Biofuels to Deploy Jatropha Hybrids for Biodiesel in India 2
(Date:4/17/2014)... A Kansas State University engineer has developed ... remotely detects improvised explosive devices. The same technique ... Dunn, the Steven M. and Kay L. Theede ... and nuclear engineering, and his research team have ... underground or in car trunks. The distance detection ...
(Date:4/17/2014)... in Europe, Asia and the United States are spreading ... impacting native honeybee populations at this time, according to ... including Nosema microsporidia and Varroa ... to these invasive pests, which suggests to us that ... and the United States currently are not necessary in ...
(Date:4/17/2014)... Current Biology on April 17 have discovered ... Brazilian insects, which represent four distinct but related species ... example of an animal with sex-reversed genitalia. , ... animals, Neotrogla is the only example in ... Yoshizawa from Hokkaido University in Japan. , During copulation, ...
Breaking Biology News(10 mins):Patented research remotely detects nitrogen-rich explosives 2East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3In sex-reversed cave insects, females have the penises 2
... Sea, harbour seals often encounter murky water that impedes ... chase prey. Extending their vibration-sensitive whiskers, the mammals are ... would be if guided by sight. Wolf Hanke and ... fascinated by how harbour seals perceive the world through ...
... the depths of the tropical rainforest not only depends on ... on the type of cooperation researchers discovered between ants and ... how the ants use chemical signals on their host tree ... plant is recognised the ants prune them to defend their ...
... earth. But how are these clouds formed? How does the ... affected by climate change? Can humans affect cloud formation to ... the health of the planet? Microbiologist William Whitman and ... think these are timely questions. They are coauthors of a ...
Cached Biology News:Seals sense shapes using their whiskers to feel wakes 2How ants tame the wilderness: Rainforest species use chemicals to identify which plants to prune 2
... Detection of the yolk protein Vtg in ... simple and sensitive biomarker for endocrine disrupting chemicals ... Vtg has become an accepted screening test for ... Vtg (rainbow trout) EIA kit is a double-antibody ...
... Laboratory (RTL) can provide you with ... ,,Microbial method development and validation ,Performance ... conditions of your GMP manufacturing environment ... offer a full spectrum of microbiological ...
... amino acid synthetic peptide whose sequence is from rat SCAMP 5. ... C - N(217) - Q - P - Q - ... - P - N - Y - T - Y - ... neutralization and control experiments with the polyclonal antibody that reacts with ...
... (gram quantities of IgY) , After we receive ... we segregate the appropriate number of chickens and ... eggs are collected (usually over a period of ... involves a total of four injections into the ...
Biology Products: