Navigation Links
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

β-actin mRNAs were amplified in 2 separate PCR reactions. All samples were run in quadruplicate. A representative example of the real-time RT-PCR quantitation of GAD67 and β-actin mRNAs expression in one individual case is shown in Figure 3. The mean threshold cycles for GAD67 and β-actin were 21.9 and 15.8 with standard deviations of 0.12 and 0.22, respectively.

Statistical Tests of Significance
Analysis of variance (ANOVA) followed by Newman-Keuls tests and Students t tests were used to analyze the results of these studies. Statistical analyses were performed using Statistica for Windows (release 5.5, Statsoft Inc., Tulsa, OK) or SPSS for Windows (version 10, SPSS Inc., Chicago, IL).


Results and Discussion
The relative abundance of GAD67 in the dorsolateral prefrontal cortex is shown in Figure 4. Analysis revealed a significant increase in GAD67 expression in the schizophrenic cohort (F(2,35) = 4.12, p = 0.02; Newman-Keuls test, schizophrenia vs. normal-elderly, p = 0.023).

The schizophrenic cohort was significantly younger than the normal-elderly group (Table 1). To determine whether age at death affected the differences between groups, the schizophrenic and normal-elderly groups were subgrouped into 2 groups of 10 subjects each that were matched for age to within 1 year of each other. Differences in the expression of GAD67 were reassessed using t tests. The same significant (p < 0.02) group difference found when analyzing the entire cohort was observed when comparing the groups of schizophrenics and normal-elderly subjects that were matched for age (Figure 4). Similar results were obtained when 2 subgroups of 10
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
9. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
10. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
11. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
Post Your Comments:
(Date:7/30/2015)... 30, 2015 According to a ... Molecular Modeling, PB/PK, PKPD, Trial Design, Toxicity Prediction, In-house, ... Clinical Trial), by End Users - Global Forecast to ... to reach USD 2,107.99 million by 2020 from USD ... Browse more than 75 ...
(Date:7/30/2015)... , July 30, 2015 Ascendis ... company that applies its innovative TransCon technology to ... top-line results from a six-month Phase 2 study ... TransCon Growth Hormone in 53 treatment-naïve, pre-pubertal children ... "We are extremely pleased with the top-line results ...
(Date:7/29/2015)... Sanofi, a global and ... quarter of 2015. CEO Olivier Brandicourt ... in different businesses. Watch video interview ... Topics covered in the interview include:  ... Performance drivers - Diabetes ...
(Date:7/29/2015)... , July 29, 2015 /PRNewswire/ - BioAmber Inc. (NYSE: ... , the Company,s Chief Financial Officer has resigned.  The ... Chief Financial Officer who retired in December 2014, as ... for a permanent successor.     Andy Ashworth ... Laurin and ensure a smooth transition.  Andy was the ...
Breaking Biology Technology:Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3
... Imaging and Printing Group and its Personal Systems Group into ... milestone in HPs journey to become the consumer ... a unified and convincing product message for every room in ... HPs consumer message into overdrive. , ,The consumer technology market ...
... School Press) takes a detailed look at how video games ... ,Authors John C. Beck and Mitchell Wade ... a lesson on how to handle the increasing number of ... competitiveness, risk-taking and everything else are shaped by video ...
... Wis. Three of the top video-game researchers will ... Thursday at the Fluno Center in Madison. , ,The ... Madison are holding an event titled Discover how Games ... rapidly growing market for games that can be applied to ...
Cached Biology Technology:HP charges toward the living room 2HP charges toward the living room 3Got Game makes big claims; can 90 million people think the same? 2Businesses and educators to preview video games for training 2Businesses and educators to preview video games for training 3
(Date:7/8/2015)... FRANKLIN LAKES , N.J. and NEW YORK ... and Company) and Guidepoint today announced ... selected start-up healthcare companies with free access to Guidepoint,s ... mentoring several start-ups that are developing cutting-edge technologies to ... of a dedicated Guidepoint research manager, each start-up entrepreneur ...
(Date:7/7/2015)... , July 7, 2015  Based on its ... & Sullivan recognizes Credence ID, LLC with the ... Company of the Year Award. Credence ID has ... in its mission of offering enrollment and identification ... As Credence ID was formed by experts from ...
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ...
Breaking Biology News(10 mins):BD and Guidepoint Team to Connect Healthcare Start-up Companies With Business and Scientific Experts 2BD and Guidepoint Team to Connect Healthcare Start-up Companies With Business and Scientific Experts 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... in the United States will receive a diagnosis of breast ... cause of cancer-related death in women. Now a new test ... patient. "Our research shows that there is a subset ... at all, other than a lumpectomy," explains Philip Bernard, M.D., ...
... free, two-day colloquium begins February 12, 2009 with the ... originator of the modern theory of evolution of species ... foundation of all modern biology. The invited speakers provide ... and empirical research. As such, the symposium emphasizes how ...
... direct chemical modifications to specific sites on recombinant proteins ... pharmaceutical industry has been developed by Carolyn Bertozzi ... Lawrence Berkeley National Laboratory and the University of California ... diabetes, can be made in bacterial systems like ...
Cached Biology News:New test for breast cancer will help guide treatment choices 2Darwin's legacy in 21st century biology 2Tailor-made recombinant proteins in mammals 2Tailor-made recombinant proteins in mammals 3Tailor-made recombinant proteins in mammals 4
BD BioCoat BD MatrigelTM Matrix 100 mm Culture Dishes for hepatocytes, tissue-culture treated polystyrene with a uniform application of MATRIGEL Matrix....
...
... internal standard for the quantification of 5-OxoETE ... is a polyunsaturated keto acid formed by ... It stimulates cytosolic calcium levels in neutrophils ... selectively stimulates the migration and degranulation of ...
... a 10.0 kb Autographa californica (AcNPV) polyhedrin ... AcNPV polyhedrin promoter and two AcNPV p10 ... a Sma I cloning site, followed by ... inverted polyhedrin promoter has been inserted containing ...
Biology Products: