Navigation Links
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

al 25 l PCR contained 5 l of the relevant cDNA (diluted 25-fold with water), 200 nM of molecular beacon for GAD67 or 100 nM for β-actin, 500 nM of forward and reverse primers, 1 U of AmpliTaq Gold DNA polymerase (Applied Biosystems), 250 M of each dNTP, 4 mM MgCl2, 50 mM KCl, and 10 mM Tris-HCl (pH 8.3). The thermal cycling program consisted of 10 min at 95C to activate the polymerase, followed by 10 cycles of 15 sec at 95C, 30 sec at 6859C (touchdown PCR, annealing temperature was decreased 1C after each cycle), and 30 sec at 72C. This touchdown step was followed by 35 cycles of 15 sec at 95C, 30 sec at 58C, and 30 sec at 72C. Fluorescence was monitored during the 58C annealing steps.

Assessment of DNA Contamination in RNA Samples
In order to measure the level of contaminating chromosomal DNA, all RNA samples not treated with reverse transcriptase and their respective cDNA samples were subjected to PCR using GAD67 primers. The products of the PCR reactions were analyzed on ethidium bromide-stained agarose gels. In contrast to their respective cDNA templates, no PCR products could be detected with gel electrophoresis when RNA samples were used. Random RNA samples (n = 10) were also subjected to real-time PCR using β-actin primers and a molecular beacon. The RNA samples showed at least 3 orders of magnitude lower amounts of the initial template molecules than their respective cDNAs (the difference in CT) between the RNA and cDNA in each sample was at least 10 cycles), demonstrating negligible amounts of genomic DNA contamination (not shown).

Dynamic Range of Real-Time PCR
To determine sensitivity of the assays, the amplification of each mRNA
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
9. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
10. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
11. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
Post Your Comments:
(Date:2/26/2015)... 2015 Protiviti ( www.protiviti.com ), a global ... Governance Portal , designed to increase the efficiency ... tasks across multiple business units and projects. The Governance ... better align their controls with the COSO ( Committee ... In response to client feedback, Protiviti has also included ...
(Date:2/26/2015)... 2015 The healthcare landscape is rapidly and radically ... Sullivan will host its 20 th Anniversary Medical ... 8 to 10, at the Hilton San Diego Resort and ... This event is a platform for global medical devices, ... disruptions and how to succeed in an uncertain future. ...
(Date:2/26/2015)... MD (PRWEB) February 26, 2015 RURO, ... provider, released FreezerPro® version 6.2 – the latest update ... FreezerPro® reputation is that of a quick and easy ... work. With the latest version, RURO engineering has enhanced ... import to move large amounts of sample records. ...
(Date:2/26/2015)... ANGELES , Feb. 26, 2015 Tauriga ... life sciences company with interests in the natural wellness ... technology, announced today that its products will be featured ... Roth Capital Partners, 27 th Annual Growth Stock ... Carlton, located at 1 Ritz Carlton Drive, ...
Breaking Biology Technology:Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 2Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 3Embrace the Future of Medical Technologies with Frost & Sullivan 2Embrace the Future of Medical Technologies with Frost & Sullivan 3RURO Releases FreezerPro® version 6.2 2SEC Compliance Featuring Products From Tauriga Sciences, Inc. at ROTH Capital Conference 2SEC Compliance Featuring Products From Tauriga Sciences, Inc. at ROTH Capital Conference 3SEC Compliance Featuring Products From Tauriga Sciences, Inc. at ROTH Capital Conference 4
... to Enroll in Phase 4 Study Initiated Pre-Registration,-, ... a,division of Wyeth (NYSE: WYE ), today ... Trial in Adults a major study in,adults of ... pneumococcal pneumonia -- the leading cause of bacterial ...
... 23 /PRNewswire-FirstCall/ - Spectral Diagnostics Inc.,(TSX: SDI) today ... with Toray,Industries Inc. of Japan to obtain the ... Toraymyxin(TM), a therapeutic for the treatment of,sepsis that ... of the,agreement, the Company will seek FDA approval ...
... InspireMD, Ltd.,announced today the enrollment of the ... stent in acute myocardial infarction. The GUARD Study,(MGuard(TM) ... evaluate,the safety and efficacy of MGuard(TM) in patients ... patients were implanted,successfully with the MGuard(TM) Coronary Stent, ...
Cached Biology Technology:Major Adult Vaccine Clinical Trial for Pneumonia Prevention Begins 2Major Adult Vaccine Clinical Trial for Pneumonia Prevention Begins 3Major Adult Vaccine Clinical Trial for Pneumonia Prevention Begins 4Major Adult Vaccine Clinical Trial for Pneumonia Prevention Begins 5Spectral to bring anti-sepsis therapy to the U.S. market 2Spectral to bring anti-sepsis therapy to the U.S. market 3InspireMD Announces Enrollment of First Patients in the MGuard(TM) Acute Myocardial Infarction Clinical Trial Data Will be Used to Confirm Initial Positive Results 2InspireMD Announces Enrollment of First Patients in the MGuard(TM) Acute Myocardial Infarction Clinical Trial Data Will be Used to Confirm Initial Positive Results 3
(Date:2/5/2015)... N.C. , Feb. 5, 2015  Marken is ... logistics company and has launched a new marketing campaign ... Logistics Organization (CLO).  The new campaign focuses on ... patient, protocol and shipments. The first headline ... , aligns Marken,s priorities with its client,s priorities. Marken ...
(Date:2/5/2015)... FRANCISCO , January 28, 2015 ... Market Analysis, Size And Segment Forecasts To 2020 has ... global IR camera market is expected to reach USD ... by Grand View Research, Inc. IR cameras help identify ... and are expected to witness surging demand in medical ...
(Date:2/5/2015)... VIEW, Calif. , Feb. 3, 2015 /PRNewswire/ ... printer technology, demand from industries such as consumer ... global market for surface mount technology (SMT) screen ... equipment to automatic control will push the adoption ... dispensers will remain steady as glue dispensers are ...
Breaking Biology News(10 mins):Marken Launches New Patient-Centric Campaign 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3
... (Sept. 22, 2011) -- Maintaining fluid balance in the ... to the mightiest of mammals. By researching recent genomic ... this intricate process to a turning point in evolution. ... Professor Emeritus, University of Lausanne, along with colleagues Romain ...
... in the bacterium E.coli that plays a critical ... has been discovered to have the shape of a seahorse ... National Laboratory (Berkeley Lab). This discovery holds far more implications ... its never-ending battle to protect you from infections by bacteria, ...
... Medical School have found that a common cancer protein leads ... It helps regulates memory formation and may be implicated in ... drives many types of solid tumors and blood cancers. The ... first revelation that cyclin E has a crucial role in ...
Cached Biology News:Fluid equilibrium in prehistoric organisms sheds light on a turning point in evolution 2Close up look at a microbial vaccination program 2Close up look at a microbial vaccination program 3Close up look at a microbial vaccination program 4Cancer protein's surprising role as memory regulator 2
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
Rabbit Anti-Mouse Ym1 Polyclonal Antibody,...
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
... may be carried out using this high ... System control and data processing is provided ... The elegant, powerful and very easy to use ... and 2000., The supplied automatic sample injector has ...
Biology Products: