( ...)Quantitation of Cabergoline at Extremely Low Plasma Concentrations with ,,, a Triple Quadrupole Mass Spectrometer

Nicola Hughes,¹ Antony Harvey,² Witold Winnik,³ Jean-Jacques Dunyach,² Maan Amad,² Maurizio Splendore,² and Gary Paul³

1Biovail Contract Research,Toronto,Ontario,Canada;
2Thermo Finnigan,San Jose,CA,USA;
3Thermo Finnigan,Somerset, NJ,USA

Systemic Plasma Analysis

The data presented here was acquired on a Thermo Finnigan TSQ Quantum mass spectrometer

Overview

Quantitation of low plasma concentrations of the pharmaceutical cabergoline is performed to demonstrate the sensitivity and selectivity of the TSQ Quantum mass spectrometer. Samples with analyte concentrations ranging five orders of magnitude are analyzed to demonstrate precision and accuracy over a linear dynamic range suitable for pharmacokinetic applications. Analysis of 50 fg of cabergoline on column in minimally treated plasma samples is performed to demonstrate the sensitivity, ruggedness, and practicality of the bioanalytical method in a complex matrix.

Introduction

Pharmaceuticals with potent activity achieve their desired therapeutic effects when administered at low doses. Consequently, their systemic plasma levels are extremely low and require highly sensitive techniques for detection. Cabergoline, a synthetic ergoline derivative with a powerful dopaminergic activity, is usually administered in 0.25 mg, biweekly doses, yielding plasma concentrations in the pg/mL level.^[1,2]

A variety of analytical methods have been employed to quantify low levels of cabergoline in plasma; these include high performance liquid chromatography (HPLC), radioimmunassay (RIA), and liquid chromatogra
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8
Related biology technology :

1. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
2. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
3. Comparison of Different Methods of mRNA Quantitation
4. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
5. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
6. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
7. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
8. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
9. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
10. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
11. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System