Navigation Links
Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

Offset: 6 V
Scan Mode: Selective Reaction Monitoring
Q2 Pressure: 1.0 mTorr argon
SRM Transitions: m/z 72→55 for acrylamide; m/z 75→58 for d3-acrylamide
Collision Energy: 13 eV
Scan Width: 1.0 u
Scan Time: 0.3 s (each SRM transition)
Q1, Q3 Resolution: Unit (0.7 u FWHM)

Results and Discussion
Prior to the acquisition of acrylamide standards, it was important to determine if there was any detectable native acrylamide contribution originating from the deuterated internal standard. As shown in Figure 1, there is no acrylamide signal observed for the m/z 72→55 SRM transition at the same retention time as the 50 ng/mL d3-acrylamide standard.

The limit of quantitation (LOQ) for acrylamide on the Finnigan TSQ Quantum Discovery was 0.25 ng/mL acrylamide or 2.5 pg on column (Figure 2). This compares favorably to LOQs previously reported by other research groups, including an 8-fold improvement over the mass LOQ by LC/ESI-MS/MS (20 pg)1 and a 40-fold improvement over the concentration LOQ on the TSQ 7000 (10 ng/mL),5 which used an LC/APCI-MS/MS method.

The calibration curve for acrylamide from 0.25 ng/mL to 2500 ng/mL is displayed in Figure 3. This calibration curve was generated using the column-switching LC method just prior to the acquisition of the food extracts data. A linear regression fit to these data using 1/x weighting yielded the following equation: y = 5.5997 104 + 0.0206125x. The correlation coefficient for this curve was r2 =0.9999, indicating excellent linearity across the four order-of-magnitude dynamic range. Table 1 lists the statistical results for the acrylamide calibration curve. At the LOQ, the accuracy, as a percent relative error, is 1.1% and the precision, as a percent coefficient of variance (%
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:11/21/2014)... Texas (PRWEB) November 21, 2014 ... is a professional and in-depth research report on ... including its definition, classification, application, and industry chain ... covers the international market analysis, including China’s domestic ... macroeconomic environment & economic situation analysis. The report ...
(Date:11/21/2014)... , Nov. 20, 2014 /CNW/ - Aequus Pharmaceuticals ... announce that it has closed a brokered private ... for approximately C$3.7 million.  Cormark Securities Inc. and ... the Brokered Offering for a syndicate of agents ... Financial Corp. (collectively the "Agents"). Concurrently with the ...
(Date:11/21/2014)... , Nov. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or ... month periods ended September 30, 2014.  Biorem,s complete 2014 third quarter ... ). Financial Summary:Three-months ended September 30, , ... share data) , 2014 , 2013 ... , 5,281 , 6,715 , ...
(Date:11/18/2014)... FL (PRWEB) November 17, 2014 ... point-of-care diagnostic tests – today announces its third ... and Prevention’s (CDC) Get Smart About Antibiotics Week ... Week is a national campaign designed to highlight ... health departments, and non-profit and for-profit partners to ...
Breaking Biology Technology:International Transformer Industry - Analysis on Applications, Development & Forecasts Now Available at ReportsnReports.com 2International Transformer Industry - Analysis on Applications, Development & Forecasts Now Available at ReportsnReports.com 3Aequus Pharmaceuticals closes $4.2 million private placement financing 2Aequus Pharmaceuticals closes $4.2 million private placement financing 3Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3
... July 24 Abaxis, Inc.,(Nasdaq: ABAX ), a medical ... for the first,fiscal quarter ended June 30, 2008., ... 7% over last year,s comparable quarter. -- Total sales ... units or 19% over last year,s ...
... hospital physicians, pulmonary and critical care clinicians, ... about overuse of antibiotics, DURHAM, N.C., July ... conferences of the American Thoracic Society and,Society of ... care physicians, and researchers are unanimous in their ...
... 24 Glasses embedded with a telescope,promise to ... to drive and do,other activities requiring sharper distance ... of these innovative glasses,over earlier devices in an ... Biomedical Optics, mailed to subscribers in print form ...
Cached Biology Technology:Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 2Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 3Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 4Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 5Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 6Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 7Abaxis Reports Financial Performance for the First Quarter of Fiscal 2009 8U.S. Clinicians Indicate a Strong Need for New Tools to Combat Sepsis 2U.S. Clinicians Indicate a Strong Need for New Tools to Combat Sepsis 3Telescope Embedded in Spectacle Lens Promises to Make Driving Easier for Visually Impaired 2Telescope Embedded in Spectacle Lens Promises to Make Driving Easier for Visually Impaired 3
(Date:11/15/2014)... -- While we may still be a few years away from ... to gain instant access to all that ailed his patients, ... tablets for monitoring and measuring our health are cropping up ... a tad Orwellian to some, but a new survey suggests ... opportunities into their healthcare regime. These are some ...
(Date:11/7/2014)... by Dr. Debra Auguste, associate professor, biomedical engineering, ... City College of New York, have identified a ... one of the most aggressive forms of breast ... a high mortality rate owing to aggressive proliferation ... options. However, Professor Auguste,s team, discovered the overexpression ...
(Date:11/6/2014)... deaths occur because of metastasis, yet progress in ... slow. , "It,s been particularly challenging to design ... research fellow in systems biology at Harvard Medical ... until after they,ve already metastasized." , Gujral and ... should help researchers better understand how metastasis begins. ...
Breaking Biology News(10 mins):Americans May Be Ready for a Brave New World of Healthcare 2Americans May Be Ready for a Brave New World of Healthcare 3Americans May Be Ready for a Brave New World of Healthcare 4Migration negation 2Migration negation 3
... has been shown to improve the neurological function ... University of Traditional Chinese Medicine, China showed that ... cells positive for 5-bromodeoxyuridine, a cell proliferation-related marker, ... growth-associated protein 43, a marker of synaptic plasticity ...
... potential is the direct current potential difference between the ... Its initial amplitude is a significant indicator of the ... well as injury current, can be modulated by direct ... electrical field are hard to define. Dr. Guanghao Zhang ...
... 6, 2013 /PRNewswire-iReach/ -- IdentiSys Inc., North America,s ... card issuance, emergency response, and access control security systems, ... world,s leader in Customer and Patient Experience Management systems. ... from Qmatic to the marketplace and will be the ...
Cached Biology News:IdentiSys brings Qmatic Q-Guide Queuing Systems to the North American market 2IdentiSys brings Qmatic Q-Guide Queuing Systems to the North American market 3
Kit containing MACS equipment and reagents for immunopurification of proteins....
... LCMSsolution software offers an intuitive, ... instrument control, diverse data handling, ... requirements. Comprehensive functions include data ... data management in both standalone ...
... a modern flow cytometer, a scientist can ... labeled with fluorescent probes. However, the emission ... cell labeling are broader than the light ... filters. Thus, the fluorescence signal of a ...
... Sigmas FLAG Immunoprecipitation kit allows a ... of an active FLAG-tagged protein. The ... gel, which is a highly specific ... resin. The use of affinity resin ...
Biology Products: