Navigation Links
Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

Offset: 6 V
Scan Mode: Selective Reaction Monitoring
Q2 Pressure: 1.0 mTorr argon
SRM Transitions: m/z 72→55 for acrylamide; m/z 75→58 for d3-acrylamide
Collision Energy: 13 eV
Scan Width: 1.0 u
Scan Time: 0.3 s (each SRM transition)
Q1, Q3 Resolution: Unit (0.7 u FWHM)

Results and Discussion
Prior to the acquisition of acrylamide standards, it was important to determine if there was any detectable native acrylamide contribution originating from the deuterated internal standard. As shown in Figure 1, there is no acrylamide signal observed for the m/z 72→55 SRM transition at the same retention time as the 50 ng/mL d3-acrylamide standard.

The limit of quantitation (LOQ) for acrylamide on the Finnigan TSQ Quantum Discovery was 0.25 ng/mL acrylamide or 2.5 pg on column (Figure 2). This compares favorably to LOQs previously reported by other research groups, including an 8-fold improvement over the mass LOQ by LC/ESI-MS/MS (20 pg)1 and a 40-fold improvement over the concentration LOQ on the TSQ 7000 (10 ng/mL),5 which used an LC/APCI-MS/MS method.

The calibration curve for acrylamide from 0.25 ng/mL to 2500 ng/mL is displayed in Figure 3. This calibration curve was generated using the column-switching LC method just prior to the acquisition of the food extracts data. A linear regression fit to these data using 1/x weighting yielded the following equation: y = 5.5997 104 + 0.0206125x. The correlation coefficient for this curve was r2 =0.9999, indicating excellent linearity across the four order-of-magnitude dynamic range. Table 1 lists the statistical results for the acrylamide calibration curve. At the LOQ, the accuracy, as a percent relative error, is 1.1% and the precision, as a percent coefficient of variance (%
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:9/21/2014)... the University of Basel in Switzerland have succeeded ... charged molecule for the first time. These extremely ... of molecular properties and may also contribute to ... The results were published in the scientific journal ... of the interaction between matter and light, is ...
(Date:9/20/2014)... USA (PRWEB) September 20, 2014 Entries ... 2015 Prism Awards for Photonics Innovation . The ... the multi-billion dollar optics and photonics industry, and is ... and photonics , and Photonics Media . , ... the versatility of photonics, said SPIE CEO Eugene Arthurs. ...
(Date:9/19/2014)...  InterMune, Inc. (Nasdaq: ITMN ) today ... to settle the litigation in the Delaware Court of ... dated as of August 22, 2014, among Roche Holdings, ... term of the Memorandum of Understanding, the Company agreed ... attached exhibit reflecting the amendment to the Company,s Schedule ...
(Date:9/19/2014)... rather than electricity, to move data would consume ... a growing concern as chips, transistor counts rise. ... circuits light emitters, modulators, and detectors ... light source for optical chips is molybdenum disulfide ... as a single, atom-thick layer. Other experimental on-chip ...
Breaking Biology Technology:Uncovering the forbidden side of molecules 2Entry deadline is 10 October for international photonics Prism Awards 2Entry deadline is 10 October for international photonics Prism Awards 3Entry deadline is 10 October for international photonics Prism Awards 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 2InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 5InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 6InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 7InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 8InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 9InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 10InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 11InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 12InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 13InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 14InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 15InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 16InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 17InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 18InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 19Toward optical chips 2Toward optical chips 3
... PRINCETON, N.J., May 1 Ranbaxy Pharmaceuticals ... voluntary recall of all lots of Nitrofurantoin (Monohydrate/Macrocrystals) Capsules, ... U.S. Although certain lots of the product were ... laboratory specifications, Ranbaxy decided to recall all the lots, ...
... May 1 /PRNewswire-Asia/ -- Yongye Biotechnology International, Inc. (OTC ... distributor of plant and animal nutrient products located in ... of the independently owned Yongye branded retail stores in ... day sales.A Yongye branded retail store in Qianjiang City ...
... 1 Tigris Pharmaceuticals, Inc., a privately held drug ... a Phase I clinical trial of GGTI-2418. GGTI-2418 ... I) that induces apoptosis by downregulating several pivotal oncogenic ... by Dr. Peter O,Dwyer, Professor of Medicine at the ...
Cached Biology Technology:Yongye Biotechnology Distributor has Record Breaking Single Store Sales Day 2Yongye Biotechnology Distributor has Record Breaking Single Store Sales Day 3First Patient Dosed in Phase I Clinical Trial of Tigris Pharmaceuticals' GGTI-2418 2First Patient Dosed in Phase I Clinical Trial of Tigris Pharmaceuticals' GGTI-2418 3
(Date:9/19/2014)... will study multifunctional and specialized spinal cord nerve ... Science Foundation grant in the amount of $680,000 ... in the Department of Biology, OU College of ... between the multifunctional and specialized spinal cord nerve ... what connections they make. Berkowitz will collaborate ...
(Date:9/19/2014)... Inc. (Nasdaq: NXTD and NXTDW) ("Nxt-ID" or the ... updates the "Wocket in Your Pocket" celebrity ad campaign, featuring former ... classes, Vinny Pazienza (PAZ). , ... actor Ciaran Hinds and Sons of Anarchy ... have been cast in Bleed for This . ...
(Date:9/18/2014)... A new GSA Bulletin study uses ... Rio Puerco and Chaco Wash in northern New Mexico, ... salt cedar and willow, investigators were able to precisely ... They then combined this data with aerial imagery, LiDAR, ... of these arroyos. , Arroyos are deep, oversized channels ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3Tree rings and arroyos 2Tree rings and arroyos 3Tree rings and arroyos 4Tree rings and arroyos 5Tree rings and arroyos 6Tree rings and arroyos 7
... and biological aspects of cellular self-organization are well-studied; less ... how their behavior and communication are affected by ... the University of California San Diego, in a paper ... of the National Academy of Sciences, have begun ...
... Japanese researchers were awarded the 2008 SNM Wagner-Torizuka ... States through 2010 as part of the two-year ... Medical College, undertaking a fellowship at Johns Hopkins ... the University of Washington, Seattle; Ryo Nakazato, Toho ...
... has clearly illustrated, the top priority for the next ... Believing that expanding the nation,s commitment to basic ... the next President can take to ensure our long-term ... questions (attached) to the moderator of tomorrow,s presidential debate. ...
Cached Biology News:UC San Diego bioengineers fill holes in science of cellular self-organization 22008 SNM Wagner-Torizuka Fellowships announced 22008 SNM Wagner-Torizuka Fellowships announced 3The Science Coalition's '10 questions' for the presidential debate 2
...
Rabbit polyclonal to SMARCA2 / BRM - ChIP Grade ( Abpromise for all tested applications). entrezGeneID: 6595 SwissProtID: P51531...
Identify and characterize Phosphoinositide (PI(4,5)P2) binding...
... These skirted micro test tubes are ... of polypropylene, these tubes have uniform walls ... nonsterile, with separate O-ring screw caps. The ... and have an operating range from -55 ...
Biology Products: