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Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

port presents data acquired on the Finnigan TSQ Quantum Discovery for the analysis of acrylamide. A simple LC/MS/MS method using the APCI source is used to measure acrylamide, via selective reaction monitoring (SRM), over a wide concentration range. A small selection of food samples was analyzed for acrylamide content following extraction with water. To preclude the need for a time-consuming solid-phase extraction procedure, a column-switching method was employed to selectively fractionate acrylamide from polar matrix interferences prior to LC/MS/MS detection.

Goals
1. Develop a sensitive and rugged LC/APCI-MS/MS assay for the analysis of acrylamide
2. Apply an on-line column-switching technique to aqueous food extracts as an alternative to solid-phase extraction (SPE) cleanup
3. Measure acrylamide content in selected food samples

Experimental

Chemicals and Reagents : Acrylamide (>99.0%) was purchased from Fluka (Buchs SG, Switzerland). 2,3,3-d3- acrylamide (98%) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). HPLC grade water was acquired from Burdick and Jackson (Muskegon, MI, USA). All chemicals were used as received without further purification.

Sample Preparation: Standards were prepared by dilution of a stock solution of 1.0 mg/mL acrylamide or 1.0 mg/mL d3-acrylamide in water. The stock solutions were stored at 4C for a period of no longer than two weeks.

Two brands of potato chips and two brands of breakfast cereals were purchased and stored at room temperature until processed. After homogenizing approximately 50 grams of a food sample, two grams were weighed into a 35 mL polypropylene centrifuge tube. Aqueous extraction of acrylamide was initiated by the addition of 20 mL water containing 1000 ng d3-acrylamide as the internal standard (final concentration = 50 ng/mL). The sample wa
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Attachments: Clip bar for tubes 10 to 19 mm in diameter Overall Dimension Height IC: 6 (15.2) Overall Dimension Length IC: 18.5 (46.9) Overall Dimension Width IC: 4 (10.2)...
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