Navigation Links
Quantitation of Acrylamide in Food Samples on the Finnigan TSQ Quantum Discovery by LC/APCI-MS/MS

port presents data acquired on the Finnigan TSQ Quantum Discovery for the analysis of acrylamide. A simple LC/MS/MS method using the APCI source is used to measure acrylamide, via selective reaction monitoring (SRM), over a wide concentration range. A small selection of food samples was analyzed for acrylamide content following extraction with water. To preclude the need for a time-consuming solid-phase extraction procedure, a column-switching method was employed to selectively fractionate acrylamide from polar matrix interferences prior to LC/MS/MS detection.

Goals
1. Develop a sensitive and rugged LC/APCI-MS/MS assay for the analysis of acrylamide
2. Apply an on-line column-switching technique to aqueous food extracts as an alternative to solid-phase extraction (SPE) cleanup
3. Measure acrylamide content in selected food samples

Experimental

Chemicals and Reagents : Acrylamide (>99.0%) was purchased from Fluka (Buchs SG, Switzerland). 2,3,3-d3- acrylamide (98%) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). HPLC grade water was acquired from Burdick and Jackson (Muskegon, MI, USA). All chemicals were used as received without further purification.

Sample Preparation: Standards were prepared by dilution of a stock solution of 1.0 mg/mL acrylamide or 1.0 mg/mL d3-acrylamide in water. The stock solutions were stored at 4C for a period of no longer than two weeks.

Two brands of potato chips and two brands of breakfast cereals were purchased and stored at room temperature until processed. After homogenizing approximately 50 grams of a food sample, two grams were weighed into a 35 mL polypropylene centrifuge tube. Aqueous extraction of acrylamide was initiated by the addition of 20 mL water containing 1000 ng d3-acrylamide as the internal standard (final concentration = 50 ng/mL). The sample wa
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:3/30/2015)... England , March 30, 2015 ... developing novel biological drugs to treat cancer, viral ... of Professor Sir John Bell , the ... Chairman of the Office for the Strategic Coordination ... with immediate effect.      (Photo: ...
(Date:3/29/2015)... , March 29, 2015  Caris Life ... data in small cell cervical cancer (SCCC) ... Molecular Intelligence®, the company,s panomic, comprehensive tumor ... mutations which may explain sensitivities and resistance ... identification of certain biomarkers may guide treatment ...
(Date:3/27/2015)... , 27. März 2015  Peter Walter ... Biomedical Science für seine bahnbrechende Arbeit ausgewählt, ... Zellkompartimenten transportiert werden, sowie für die Entschlüsselung ... Reaktion auf Stress nutzen, der mit der ... ist Professor für Biochemie an der University ...
(Date:3/27/2015)... SHANGHAI , March 27, 2015 ... a leading open-access R&D capability and technology platform ... device industries, announced today that an Investigational New ... monoclonal antibody for rheumatoid arthritis has been accepted ... Administration (CFDA).  In September 2012, ...
Breaking Biology Technology:Immunocore Appoints Professor Sir John Bell to its Board of Directors 2Immunocore Appoints Professor Sir John Bell to its Board of Directors 3Immunocore Appoints Professor Sir John Bell to its Board of Directors 4Immunocore Appoints Professor Sir John Bell to its Board of Directors 5Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 2Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 2Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 4Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 2Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 3
... Breweries announced a new partnership, which will create a joint venture ... , ... Lexington, KY (Vocus) March 11, 2009 -- ... announced a new partnership, which will create a joint venture company ...
... Legislation as Pro-Patient and Pro-Pharmaceutical InnovationARLINGTON, Va., ... Pharmaceutical Association (GPhA) released the following statement ... Jaeger regarding the introduction of H.R. 1427, ... and Access to Life-Saving Medicine Act.""For the ...
... for rural Indians - Leverages Satyam,s earlier success with ... (NYSE: SAY ) a leading global consulting ... was honored, along with the LV Prasad Eye Institute, ... program, which provides expert and affordable eye care to ...
Cached Biology Technology:Alltech, Mitsui and Asahi Breweries Create New Venture 2Alltech, Mitsui and Asahi Breweries Create New Venture 3GPhA Statement on the Introduction of the Bipartisan Biogenerics Consensus Bill: 'Promoting Innovation and Access to Life-Saving Medicine Act' 2Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 2Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 3Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 4
(Date:3/12/2015)... Va. , March 12, 2015 IriTech, ... and a member of the Texas Instruments Design Network, ... Naidu has demonstrated its IriShield USB MK2120U device ... scanning facility for pension distribution. The ... assembled to be marketed in India ...
(Date:3/11/2015)... and Markets ( http://www.researchandmarkets.com/research/3p32qm/access_control ) has announced ... Product, Application & By Geography - Global Forecast & ... This report predicts that the access control ... with an estimated CAGR of 10.6%. The ... as contact cards & readers, contactless cards & readers, ...
(Date:3/10/2015)... 2015   Tute Genomics , pioneer in affordable, ... next-generation sequencing company PrimBio Research Institute to ... gene panel interpretation. PrimBio, a certified Life ... current technologies to support the work of experts on ... two types of exome sequencing services: 1) Targeted Exome ...
Breaking Biology News(10 mins):Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2PrimBio Research Selects Tute as Whole Exome Analysis Provider, Creating Integrated, End-to-End Solution for Next-Generation Sequencing & Data Analysis 2PrimBio Research Selects Tute as Whole Exome Analysis Provider, Creating Integrated, End-to-End Solution for Next-Generation Sequencing & Data Analysis 3
... E or a combination of the supplements had a similar ... supplements, according to data from a randomized controlled trial in ... the National Cancer Institute. Epidemiological studies have suggested ... and thus antioxidants, may have a lower risk of cancer. ...
... has the honour of becoming the first student from ... competition that pitted engineering students and young professionals from ... the Institution of Engineering and Technology (IET), UK, the ... Mechanical & Aerospace Engineering beat three other contestants in ...
... Is Sensitive to Irradiation Researchers led by ... irradiation damages fat tissue. They report their findings in ... of Pathology . Radiation therapy directed ... of tissues such as fat tissue has often been ...
Cached Biology News:Vitamins C and E and beta carotene again fail to reduce cancer risk in randomized controlled trial 2NTU undergrad is first Singapore champion of an internationally acclaimed engineering competition 2NTU undergrad is first Singapore champion of an internationally acclaimed engineering competition 3Tips from the American Journal of Pathology 2Tips from the American Journal of Pathology 3Tips from the American Journal of Pathology 4Tips from the American Journal of Pathology 5