Navigation Links
Quantification of the Purinergic Receptor P2X3 using ICAT and ,,, Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

r. The supernatant was removed and the pellet resuspended in 1 M NaCl PBS by sonication and then pelleted again at 100,000 xg for 1 hour.

The resulting cell membranes were solubilized by sonication in 500 L of buffer containing 6 M urea and 0.1% SDS in 50 mM TRIS pH 8.0. Figure 1. Fluidic path configuration of the orthogonal 2D LC-MS/MS system used for analysis of complex protein mixtures. ICAT Labeling and Proteolysis The cell membrane suspension was reduced with TCEP (5 mM) for 30 minutes at room temperature. The sample was diluted to 3 mL to reduce the urea concentration and then split into two fractions prior to alkylation with ICAT. Fraction A (2 mL) was labeled with d0-ICAT and Fraction B (1 mL) with d8-ICAT. Fractions A and B were alkylated with ICAT reagent for 1 hour in the dark at room temperature. After alkylation, both fractions were recombined to yield a theoretical ratio of 1: 0.5 for d0: d8. A total of 90 g of trypsin was added to the mixture followed by overnight incubation at 37C.

After digestion, the samples were acidified with 40 L of formic acid and 5 L of TFA to a pH < 2. A cloudy precipitate was formed which was removed by centrifugation. The supernatant was removed and examined by RP-HPLC to verify the presence of peptides prior to analysis by 2D LC-MS/MS. Figure 2. Graphical user interface for programming of the 2D LC gradients (SCX and RP) and MS acquisition parameters using ProteomeX. Orthogonal 2D LC- MS/MS
Orthogonal 2D LC-MS/MS was performed
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Quantification made easy
2. Quantification of siRNA Silencing Efficiency Using the LightCycler System
3. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
4. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
5. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
6. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
7. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
8. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
9. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
10. Gene-Specific Primers for NMDA Receptor Subunits
11. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
Post Your Comments:
(Date:4/24/2015)... 24, 2015 DryLet, LLC a ... applications such as animal waste reduction, bioremediation, wastewater ... in wastewater treatment plants and restaurant kitchen settings, ... the National Restaurant Association’s NRA Show 2015, in ... of the company will be available to share ...
(Date:4/24/2015)... Telbec/ - Mirego, leader in mobile strategies and solutions, is ... app extensions for the all new Apple Watch. Made in ... and MediaMiser, these extensions will reinvent the way users receive ... wrist use.  Available today in ... tactile smartwatch that makes for an excellent buddy for business, ...
(Date:4/24/2015)... April 24, 2015 Seoul Semiconductor ... for various applications in the lighting market. ... installations of this technology in emerging markets such ... a wide range of applications such as streetlight ... decorative lighting. , First launched in 2005 the ...
(Date:4/23/2015)... 23, 2015 The Thomas Brain ... conduct a groundbreaking pilot study to see if ... in as little as three to six months. ... and nutritional medicine—will aggressively target and simultaneously treat ... our aging brain. These include oxidative stress, neuroinflammation, ...
Breaking Biology Technology:DryLet to Showcase Bio-React at the National Restaurant Association’s NRA Show 2015 May 16-19, 2015 in Chicago, Illinois 2Familiprix, OMSignal and MediaMiser apps available today for Apple Watch 2Acrich Technology from Seoul Semiconductor Adopted in Installations Worldwide 2New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3
... , NEW YORK, Aug. 5 ... biopharmaceutical company focused on the acquisition, development and commercialization ... of life-threatening diseases, including renal disease and cancer (the ... ended June 30, 2009. , , ...
... , , SAN DIEGO, ... ), a biopharmaceutical company focused on in-licensing, developing and commercializing ... today reported financial results for the three and six months ... the second quarter of 2009, Cadence submitted its New Drug ...
... CARLISLE, Mass., Aug. 5 CapitalBio ... of a fully integrated microarray solution platform. CapitalBio ... and advanced microarray instrument systems, in collaboration with ... instrumentation software, now offers a platform that combines ...
Cached Biology Technology:Keryx Biopharmaceuticals, Inc. Announces Second Quarter 2009 Financial Results 2Keryx Biopharmaceuticals, Inc. Announces Second Quarter 2009 Financial Results 3Keryx Biopharmaceuticals, Inc. Announces Second Quarter 2009 Financial Results 4Keryx Biopharmaceuticals, Inc. Announces Second Quarter 2009 Financial Results 5Keryx Biopharmaceuticals, Inc. Announces Second Quarter 2009 Financial Results 6Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 2Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 3Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 4Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 5Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 6Cadence Pharmaceuticals Reports Second Quarter 2009 Financial Results 7CapitalBio Corporation and VigeneTech Inc. Have Announced the Launch of a Fully Integrated Microarray Solution Platform 2CapitalBio Corporation and VigeneTech Inc. Have Announced the Launch of a Fully Integrated Microarray Solution Platform 3
(Date:4/10/2015)... 2015 Research and Markets ( ... "Security Competitive Profiles - NEC" report to their ... NEC will continue to supply a range of IT ... a company focus on the development of a Big ... opportunities in the Asia-Pacific region ...
(Date:4/2/2015)... , April 2, 2015 At its ... Salt Lake City , the American College of ... five new directors to its Board.  Members of the ... ACMG and for forming and advancing its policies and ... genetics profession. "It,s an eventful time in ...
(Date:3/31/2015)... March 31, 2015  Elephant Talk Communications Corp. (NYSE ... provider of Software Defined Network Architecture (ET Software DNA® ... revenue of approximately $20.4 million for the year ended ... million for the year ended December 31, 2013. ... Company had completed its multi-year transition away from its ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... a clear agenda when it comes to selecting a mating ... preferring large females, they are likely to have more and ... Mattle and Tony Wilson from the Zoological Museum at the ... just been published online in Springer,s journal Behavioral Ecology ...
... two common dietary oil supplements affect body composition suggests ... in obese postmenopausal women with Type 2 diabetes. ... cooking oil, and conjugated linoleic acid (CLA), a compound ... has been associated with weight loss in previous studies. ...
... Tiny flying machines can be used for everything ... simply making smaller versions of planes and helicopters ... Carolina State University are mimicking nature,s small flyers ... maneuverability and performance. Small flyers, or micro-aerial ...
Cached Biology News:Male seahorses like big mates 2Two dietary oils, two sets of benefits for older women with diabetes 2Two dietary oils, two sets of benefits for older women with diabetes 3Two dietary oils, two sets of benefits for older women with diabetes 4Robo-bats with metal muscles may be next generation of remote control flyers 2
PKC (pSer345), phospho-specific...
Anti-ADAMTS-4, disintegrin-like domain Immunogen: Synthetic peptide - KLH conjugated Available Date: 38569...
Rabbit polyclonal to Streptococcus Enterotoxin C ( Abpromise for all tested applications)....
... 100 g monoclonal antibody which can ... V from heart mitochondria. Also included are ... control immunocapture. The Complex V immunocapture kit ... (E.C. 3.6.3.14) from small amounts of tissue. ...
Biology Products: