Navigation Links
Quantification of Nucleosomes in Serum,,,by the Cell Death Detection ELISAplus

proved the comparability of the results by establishing a standard curve, standardizing the test performance and the preanalytic handling of blood samples [16]. Despite the presence of interfering factors in circulation, the concentration of nucleosomes in serum might reflect the rate of cell death particularly in active, well perfused tumors and the rate of induced cell death in individuals during antitumor therapy.

Materials and Methods

The Cell Death Detection ELISAplus is based on a quantitative sandwich-enzyme-immunoassay principle: Monoclonal mouse antibodies directed against DNA (single-stranded [ss] and double-stranded [ds] DNA) and histones (H1, H2A, H2B, H3 and H4) detect specifically mono- and oligonucleosomes.

The sample was placed in a streptavidin-coated microtiter plate and incubated with a mixture of biotinylated anti-histone antibodies, peroxidase-labeled anti-DNA antibodies and incubation buffer (1% BSA, 0.5% Tween, 1mM EDTA in PBS) for two hours. The antibodies were bound to the histone- and DNA-component of the nucleosomes, and the immunocomplexes were fixed to the microtiter plate by streptavidin-biotin interaction. After the incubation period, unbound antibodies were removed by a washing step. The peroxidase-linked complexes retained were incubated with ABTS (2,2-Azino-di[3-ethylbenzthiazoline-sulfonate]) for 30 minutes, resulting in color development proportional to the number of nucleosomes captured in the antibody sandwich. Quantification of the nucleosomes was performed by photometrical determination of the absorbance at 405 nm against substrate solution as blank (reference wavelength 492 nm). Serum was used as matrix. One to two hours after venipuncture, the sample was centrifuged, treated with 10mM EDTA (pH 8) and stored at -20 C. After thawing, the samp
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Quantification made easy
2. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
3. Quantification of siRNA Silencing Efficiency Using the LightCycler System
4. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
5. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
6. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
7. In Situ Cell Death Detection Kit
8. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
9. A New PCR-based Mycoplasma Detection Method
10. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
11. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
Post Your Comments:
(Date:7/24/2014)... leader Yung-Eun Sung has announced that they have ... graphenes which can be applied as high performance ... Sung is both a group leader at the ... Science* (IBS) and a professor at the Seoul ... with regards to the development of relative simplicity, ...
(Date:7/24/2014)... DUBLIN , July 24, 2014 ... the "Global Genomics and Proteomics Analytical Instruments Market ... http://photos.prnewswire.com/prnh/20130307/600769 Genomics is the ... is the study of the structure and functions of ... and bioinformatics tools and technology. Genomics involves the mapping ...
(Date:7/24/2014)... July 24, 2014 2014 Deep ... Industryā€¯ is a professional and in-depth research report ... Potassium Sulphate basic information, including its definition, ... overview. This research covers the international market analysis, ... industry analysis covering macroeconomic environment & economic situation ...
(Date:7/24/2014)... Francisco, CA (PRWEB) July 24, 2014 ... http://www.draperuniversity.com , is proud to announce that ... from 17 different countries will converge on the ... Valley for an intensive, experiential program focused on ... The highly selective school, currently running its sixth ...
Breaking Biology Technology:Global Genomics and Proteomics Analytical Instruments Market 2014-2018: Key Vendors are Agilent, Bio-Rad , F. Hoffmann, La Roche, Illumina, PerkinElmer and Thermo Fisher Scientific 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 2Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 3
... Abstract , ... in the biological sciences. In drug discovery research, immunoassays ... culture supernatants of treated cells. However, scientists often avoid ... cumbersome manipulations. Applied Biosystems has developed a singlestep, mix-and-read ...
... Purpose , ... of protease inhibitor (PI) and non-nucleoside reverse transcriptase inhibitor (NNRTI) ... of assessing inter-individual and intra-individual metabolism variability. To be of ... to monitor these drugs is fast and easy to perform, ...
... Purpose , ... plays an important role in a number of functions, including the ... characterized by impaired, reduced or total absence of peroxisomes in cells, ... and hexacosanoic acids, in plasma. Some variants of these disorders are ...
Cached Biology Technology:Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System 2Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System 3Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System 4Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System 5A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors) 2A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors) 3A simple and rapid LC-MS/MS method for the simultaneous determination of nine antiretroviral drugs commonly used in Europe (protease Inhibitors and non-nucleoside reverse transcriptase inhibitors) 4A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders 2A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders 3A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders 4
(Date:7/24/2014)... NY - July 24, 2014 -- Keryx Biopharmaceuticals, Inc. ... the long-term, randomized, active control Phase 3 study of ... phosphate binder, for the treatment of hyperphosphatemia in patients ... study (PhosphatE binding and iRon delivery with FErric CiTrate ... Journal of the American Society of Nephrology ( ...
(Date:7/24/2014)... Scientists at The New York Stem Cell Foundation ... creating a viable cell replacement therapy for multiple ... For the first time, NYSCF scientists generated induced ... of patients with primary progressive multiple sclerosis and ... these stem cells into becoming oligodendrocytes, the myelin-forming ...
(Date:7/24/2014)... average over a 35 year period in which the ... of humans on declining animal numbers. This decline matters ... spiders, crustaceans, slugs and worms bring to our day-to-day ... for nutrient cycling, water filtration and human health. , ... by UCL, Stanford and UCSB, focused on the demise ...
Breaking Biology News(10 mins):Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 4NYSCF scientists 1 step closer to cell therapy for multiple sclerosis patients 2Invertebrate numbers nearly halve as human population doubles 2Invertebrate numbers nearly halve as human population doubles 3
... to biobanks vast collections of human tissue samples that ... have a right to basic information about how their donations ... a new paper. The idea behind biobanks is that ... to medical records and other health information, can yield discoveries ...
... and terrorist plots are usually the preserve of Hollywood ... is the stuff of real life. As controversy about ... new paper argues for a complete overhaul of current ... Reconfiguring Global Responses to Influenza, by Dr Paul Forster, ...
... modify their body movements to be in tune with others, ... to be in synchrony with their peers. For example, we ... unison at the end of a concert. This phenomenon is ... the RIKEN Brain Science Institute report today that pairs of ...
Cached Biology News:Safeguards needed for tissue donors 2Pandemic controversies: The global response to pandemic influenza must change 2Primates too can move in unison 2
... special preparation of bovine pancreatic DNase I that ... DNA molecules. In the presence of Mn2+ and ... breaks at random sites in DNA. The average ... by the enzyme concentration and incubation time. This ...
... protease from human rhinovirus (HRV 3C) is ... line of restriction grade proteases. The recombinant ... protein, which recognizes the same cleavage site ... 22 KDa size of the protease, optimal ...
... Thermo Hybaid's unique Electro-4 design allows ... the Electro-4 it is possible to run ... tank, by stacking up to four,horizontal gels. ... adjustable according to the number of gels ...
... The IEC Centra CL3 series ... in typical research and clinical applications ... broad accessory package includes quick connect ... An advanced yet intuitive interface allows ...
Biology Products: