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Quantification made easy

non-proteins. Additives such as EDTA, ammonia sulfate or Triton X-100 in particular are incompatible with the test. Bicinchoninine acid assay 562nm (BCA)
This test represents a highly regarded alternative to the Lowry assay. It is easier to carry out and sensitivity can be varied using different temperatures. Furthermore, the dye complex is very stable. However, this test is highly susceptible to interference, although on the positive side, its insensitivity to detergents is similar to that of the Lowry method. Bradford assay 595nm
This method is twice as sensitive as the Lowry or BCA test and is thus the most sensitive quantitative dye assay. It is the easiest to handle and most rapid method and has the additional advantage that a series of reducing substances (e.g. DTT and mercaptoethanol), which interfere with the Lowry or BCA test, have no adverse effect on results.However, it is sensitive to detergents. The main disadvantage is that identical amounts of different standard proteins can cause considerable differences in the resulting absorption coefficients. 3. Bacterial cell density The density of bacterial suspensions may be measured photometrically at 595 nm without dyes having to be added. This applies e.g. to the preparation of competent cells (i.e. cells, which are able to absorb plasmid DNA), that must be in a specific phase of growth.



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