Navigation Links
Quantification made easy

io A260/A230 should be approximately 2.2. 2. Protein determination

The protein content of a preparation can be determined on the basis of various different analytical procedures. Evaluation can be carried out via factor or via a calibration curve, with up to ten standards in the BioPhotometer. Absorption measurement at 280 nm (A280)
A280 method may be used with in concentrations of up to approximately 4 mg/ml (3.0 A). This method is simple and rapid, but may be disturbed by the parallel absorption of non-proteins (e.g. DNA). Unlike the colorimetric process, this method is less sensitive and requires higher protein concentrations and should thus be used with pure protein solutions. In addition to the direct absorbance display, evaluation is possible with the BioPhotometer via the Warburg formula or via standard. Colorimetric determination (dye tests)
Protein samples often consist of a complex mixture of many different proteins. The quantitative detection of the protein content is usually achieved on the basis of the reactions shown by functional groups of the proteins to dye-forming reagents. The intensity of the dye correlates directly with the concentration of the reacting groups and can be measured exactly. Lowry assay 595nm
Specialist literature contains a multitude of modifications for the Lowry assay. The principal target is to reduce the high susceptibility to interference. In comparison to the pure Biuret assay, the sensitivity of this assay has greatly increased. However, the Lowry method is adversely affected by a wide range of
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
2. Quantification of siRNA Silencing Efficiency Using the LightCycler System
3. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
4. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
5. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
6. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Quantification made easy

(Date:7/30/2014)... Mass. , July 30, 2014  Decision ... pharmacy and medical directors in the ... drivers of biosimilar market penetration will be the ... organizations (MCOs) and the expected lower cost to ... for biosimilars that meet their pricing expectations in ...
(Date:7/30/2014)... (PRWEB) July 30, 2014 Selexis ... for biologic drug discovery and mammalian cell line development, ... CHO-Mplus Libraries ™ will be presented at the 10th ... being held, September 8 – 10, 2014 at the ... , The following abstract will be presented during ...
(Date:7/30/2014)... Wellesley, MA (PRWEB) July 30, 2014 ... report, Global Markets for Enzymes in Industrial Applications ... to reach to nearly $7.1 billion by 2018, registering ... Maximum growth is estimated to be recorded in the ... the forecast period. , Enzyme technology has influenced almost ...
(Date:7/30/2014)... 2014 Researchers often use microelectronic devices ... such devices can do much more – perhaps ... researchers have looked to patterned assemblies of proteins ... engineering to characterize – and potentially control – ... envisioned to recreate animal and human physiological functions ...
Breaking Biology Technology:Surveyed U.S. Payers Expect Cost Savings to MCOs and Patients to be the Strongest Drivers of Biosimilar Uptake 2Surveyed U.S. Payers Expect Cost Savings to MCOs and Patients to be the Strongest Drivers of Biosimilar Uptake 3Selexis SA to Present New Data and a Case Study at IBC’s Cell Line Development and Engineering Conference 2Selexis SA to Present New Data and a Case Study at IBC’s Cell Line Development and Engineering Conference 3Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 2Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4
... (Amex: UPI ),a medical device company that ... dysfunctions, today reported financial,results for the third quarter of ... the third quarter of fiscal 2009 were $3.4 million ... "As we discussed in conjunction ...
... - Victhom Human Bionics Inc., "Victhom" (TSX:VHB), announced ... Mark approval for its Neurostep(TM) System, the first ... on peripheral nerves. CE Mark represents regulatory approval ... any of the Member States of the European ...
... Pharmaceutical Inc. (Nasdaq: BMRN ) announced today ... BioMarin marketing approval for Naglazyme(R) (galsulfase) for the treatment ... pleased to receive marketing approval for Naglazyme in Brazil ... VI patients in the world," said Stephen Aselage, Senior ...
Cached Biology Technology:Uroplasty Reports Third Quarter Fiscal 2009 Results 2Uroplasty Reports Third Quarter Fiscal 2009 Results 3Uroplasty Reports Third Quarter Fiscal 2009 Results 4Uroplasty Reports Third Quarter Fiscal 2009 Results 5Uroplasty Reports Third Quarter Fiscal 2009 Results 6Uroplasty Reports Third Quarter Fiscal 2009 Results 7Uroplasty Reports Third Quarter Fiscal 2009 Results 8Uroplasty Reports Third Quarter Fiscal 2009 Results 9Uroplasty Reports Third Quarter Fiscal 2009 Results 10Uroplasty Reports Third Quarter Fiscal 2009 Results 11Victhom Human Bionics Receives CE Mark for the Neurostep(TM) System, the World's First Fully Implantable Closed-Loop Neuromodulation Therapy for Treatment of Foot Drop 2Victhom Human Bionics Receives CE Mark for the Neurostep(TM) System, the World's First Fully Implantable Closed-Loop Neuromodulation Therapy for Treatment of Foot Drop 3Naglazyme Approved by Brazil's National Health Surveillance Agency 2Naglazyme Approved by Brazil's National Health Surveillance Agency 3Naglazyme Approved by Brazil's National Health Surveillance Agency 4
(Date:7/30/2014)... across-the-board increase in Impact Factors for its molecular ... Reports (Thomson Reuters, 2014) show that Clinical ... in Biochemistry , Biochemical Society Transactions ... in their Impact Factors. The journals are owned ... Press Limited. , 2014 Impact Factors (2013 Impact ...
(Date:7/30/2014)... Americans make better decisions about what they eat, the ... significant changes to the Nutrition Facts label found on ... in Chemical & Engineering News (C&EN), the ... the suggested updates and the fight that has ... points out that while the Nutrition Facts label has ...
(Date:7/30/2014)... from Bjorn Brembs, professor of neurogenetics at the Institute ... figure allowing readers and reviewers to run the underlying ... important leap forward for scientific publishing, by demonstrating a ... scholarly output. , Figure 3 in fact doesn,t really ... to F1000Research , and the figure is generated ...
Breaking Biology News(10 mins):Across-the-board Impact Factor increases for Portland Press Limited 2F1000Research brings static research figures to life 2
... Chemical products, energy sources, oils, paints, and more from biomass ... thought can be obtained. Aware of this, engineer Mara Gonzlez ... as the most suitable processing techniques and technologies for refining ... organic natural resources and thus substitute them for fossil fuels ...
... This release is available in French ... resources, the National Research Council of Canada, the University ... methane-eating bacteria survive in a highly unique spring located ... Lyle Whyte, McGill University microbiologist explains that the Lost ...
... a question asked by marine scientists from the Gulf of Mexico ... and marine habitat once it has been damaged or even killed? ... may be a cheap and simple solution that can be used ... was carried out by Dr Graham Forrester, from the University of ...
Cached Biology News:Ph.D. thesis opens doors to obtaining chemical products and materials using biomass as raw material 2Ph.D. thesis opens doors to obtaining chemical products and materials using biomass as raw material 3Could life survive on Mars? Yes, expert says 2Coral transplantation the simple and cheap solution to reef restoration 2
Goat polyclonal to PINX1 ( Abpromise for all tested applications). entrezGeneID: 54984 SwissProtID: Q548A5...
... membrane-filtered isotonic and enzyme-free aqueous formulations ... agents in either Ca2+- and Mg2+-free ... Ca2+- and Mg2+-free phosphate-buffered saline for ... support substrates and each other for ...
...
... Kit Components , 25 g Blocking Reagent , ... 10 ml T7 Elution Buffer , 0.2 ml ... , 0.2 ml BLT5615 glycerol stock , 1010 ... Negative Control Lysate , 50 l S-protein HRP Conjugate ...
Biology Products: