Navigation Links
QuantiTect SYBR Green RT-PCR Kit

In one-step quantification of RNA using the QuantiTect SYBR Green RT-PCR Kit, sensitivity is enhanced by combining HotStarTaq DNA Polymerase with a unique blend of Sensiscript and Omniscript Reverse Transcriptases. Sensiscript RT is designed for use with very small amounts of RNA, while Omniscript RT provides highly efficient reverse transcription with RNA amounts greater than 50 ng. The combination of both of these high-affinity reverse transcriptases ensures highly efficient, sensitive, and reproducible reverse transcription over a wide range of template amounts (see figure "High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit"). High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit One-step RT-PCR of a cyclophilin fragment from 100 ng, 1 ng, and 10 pg of total HeLa RNA. Twelve samples were amplified in parallel from each template amount. Coefficient of variation (CV) of the CT value was below 1.1% for all 3 template amounts. Reactions were carried out on the ABI PRISM 7700 Detection System.


'"/>

Source:


Page: All 1

Related biology technology :

1. QuantiTect Probe PCR Kit
2. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
3. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
4. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
5. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
6. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
7. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
8. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
9. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
10. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
11. Brilliant Core Reagent Kits for QPCR and QRT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/27/2016)... RESEARCH TRIANGLE PARK, N.C. , April 27, ... ) announced today that Martine Rothblatt , Ph.D., ... provide an overview and update on the company,s business ... Care Conference. The presentation will take place ... Time, and can be accessed via a live webcast ...
(Date:4/27/2016)... , ... April 27, 2016 , ... ... Jon Clark has joined the company as an Expert Consultant. Mr. Clark ... industry collaborations and managing the development of small molecule monographs based on analytical ...
(Date:4/27/2016)... (PRWEB) , ... April 27, 2016 , ... ... for simultaneous preclinical PET (Positron Emission Tomography) and MRI (Magnetic Resonance Imaging) in ... understanding disease and testing novel treatments in small animal subjects. Simultaneous PET/MRI imaging ...
(Date:4/27/2016)... MIAMI (PRWEB) , ... April 27, 2016 , ... ... a mutual endorsement of an Asia-Pacific Symposium as other research and development initiatives for ... of Santiago officials and top Global Stem Cells Group executives began meeting to establish ...
Breaking Biology Technology:
(Date:4/28/2016)... FRANCISCO and BANGALORE, India , ... of EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... provider, today announced a global partnership that will ... way to use mobile banking and payment services. ... is a key innovation area for financial services, but it ...
(Date:4/28/2016)... April 28, 2016 First quarter 2016:   ... 966% compared with the first quarter of 2015 The ... 589.1 M (loss: 18.8) and the operating margin was 40% (-13) ... Cash flow from operations was SEK 249.9 M (21.2) ... guidance is unchanged, SEK 7,000-8,500 M. The operating margin ...
(Date:4/26/2016)... India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a product ... and Onegini today announced a partnership to integrate ... solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... to provide their customers enhanced security to access ...
Breaking Biology News(10 mins):