Navigation Links
QuantiTect SYBR Green RT-PCR Kit

In one-step quantification of RNA using the QuantiTect SYBR Green RT-PCR Kit, sensitivity is enhanced by combining HotStarTaq DNA Polymerase with a unique blend of Sensiscript and Omniscript Reverse Transcriptases. Sensiscript RT is designed for use with very small amounts of RNA, while Omniscript RT provides highly efficient reverse transcription with RNA amounts greater than 50 ng. The combination of both of these high-affinity reverse transcriptases ensures highly efficient, sensitive, and reproducible reverse transcription over a wide range of template amounts (see figure "High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit"). High Reproducibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit One-step RT-PCR of a cyclophilin fragment from 100 ng, 1 ng, and 10 pg of total HeLa RNA. Twelve samples were amplified in parallel from each template amount. Coefficient of variation (CV) of the CT value was below 1.1% for all 3 template amounts. Reactions were carried out on the ABI PRISM 7700 Detection System.


'"/>

Source:


Page: All 1

Related biology technology :

1. QuantiTect Probe PCR Kit
2. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
3. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
4. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
5. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
6. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
7. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
8. Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
9. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
10. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
11. Brilliant Core Reagent Kits for QPCR and QRT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/9/2018)... ... August 08, 2018 , ... ActX introduces GenoACT(sm), a ... updated by a talented scientific team that includes geneticists, PharmD’s, genetic counselors, and ... Information on the medical impact of a patient’s genetic variants , 2. Educational ...
(Date:8/9/2018)... ... August 09, 2018 , ... Rockland Immunochemicals, ... Dr. Birte Aggeler, Director of Antibody Development at Bio-Techne, composed a peer-reviewed article ... published online on August 9, 2018 and will be printed in the September ...
(Date:8/1/2018)... ... August 01, 2018 , ... ... announces research results on the significant impact of a proprietary biofield energy ... , The preclinical research assessed biomarkers for systemic and organ-specific inflammation, as ...
(Date:7/27/2018)... ... July 27, 2018 , ... ... and representatives of the Biotechnology Innovation Organization (BIO) met with U.S. Environmental ... “Proposal for a Toxic Substances Control Act (TSCA) Inventory Representation and Equivalency ...
Breaking Biology Technology:
(Date:7/31/2018)... Calif. (PRWEB) , ... July 31, 2018 , ... ... to connect seamlessly with LabArchives , the leading cloud-based Electronic Lab Notebook (ELN) ... and LabArchives enables researchers to store all of their research data records in a ...
(Date:7/28/2018)... ... July 28, 2018 , ... Inthirani Arul, an evidence-based ... treated nutraceutical to combat inflammation and autoimmune disorders. , Biomarkers for immune function, ... , Over 110% increase in overall immunity as seen by ...
(Date:7/26/2018)... ... ... The Conference Forum has confirmed the agenda for the 5th Annual Mobile in ... Copley Place in Boston, MA. , Daniel Karlin, MD, Head of Experimental Medicine, Informatics, ... the discussion on how and when to implement mobile/digital technology in clinical trials and ...
Breaking Biology News(10 mins):