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Quality assurance of RNA derived from,,,laser microdissected tissue samples,,,obtained by the PALM MicroBeam System,,,using the RNA 6000 Pico LabChip kit.

transcriptome to various external factors influencing the cellular behavior. Especially in clinical research there is the need for methods that allow expression analysis from very specific cells. Prerequisite for meaningful results are samples of high purity without contamination from unwanted cells that could potentially interfere with detection of important transcripts. Laser-assisted microdissection with the PALMMicroBeam system allows isolation of individual cells in a simple and fast way. Using this technology, researchers are able to procure pure cell populations from specific areas of tissue sections for isolation of DNA, RNA, and proteins with visual control using a microscope. An UV-A-laser-mediated process dissects selected specimen from various sources and transfers them contact free directly into collection vessels for subsequent extraction (see figure 1). Together with novel methods for linear RNA amplification it is possible to perform gene expression profiling analyses even from very limited amount of cell material. The most

critical parameter for the success of such an experiment is the integrity and purity of the RNA.
Experimental case study
Tissue preparation and microdissection
Snap-frozen mouse liver tissue stored at 80 C was cut in 7 m serial sections on a cryotome at 25 C. The sections were transferred to PALM MembraneSlides (1mm glass slides covered with a 1.35 m thin Polyethylene-naphthalate- membrane to facilitate the laser pressure catapulting procedure) and air dried for 10 seconds. After a fixation step of 5 minutes in 70% ethanol at -20 C and a short wash in RNase free water (10 seconds) the sections were further processed according to standard histochemical procedures. Depending on the subsequent tests eith
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