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Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Plasmids from all kits and conditions transformed strains equivalently with high efficiency. However, we did notice differences when we sequenced plasmids derived by different purification methods (Table 6). Notably, Aurum preps performed the best under these conditions.


Conclusions
Purified plasmid DNA from the new Aurum miniprep and midiprep kits was of high quality and reproducibly performed very well in restriction digestions, bacterial and yeast transformations, and automated fluorescent DNA sequencing reactions. The Aurum kits also equaled or outperformed other commercially available plasmid purification kits for overall yield and quality of plasmid.


Reference
Gietz RD and Woods RA, High efficiency transformation with lithium acetate, In Johnston JR (ed), Molecular Genetics of Yeast: A Practical Approach, Oxford University Press, New York, 121134 (1994)

DH5α and DH10B are trademarks of Life Technologies. QIAprep is a trademark of QIAGEN. Strataprep, XL1-Blue, XL2-Blue, and XL10-Gold are trademarks of Stratagene. Wizard Plus and SV Plus are trademarks of Promega.


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