( Plasmids from all kits and condi...)Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Plasmids from all kits and conditions transformed strains equivalently with high efficiency. However, we did notice differences when we sequenced plasmids derived by different purification methods (Table 6). Notably, Aurum preps performed the best under these conditions.

Conclusions
Purified plasmid DNA from the new Aurum miniprep and midiprep kits was of high quality and reproducibly performed very well in restriction digestions, bacterial and yeast transformations, and automated fluorescent DNA sequencing reactions. The Aurum kits also equaled or outperformed other commercially available plasmid purification kits for overall yield and quality of plasmid.

Reference
Gietz RD and Woods RA, High efficiency transformation with lithium acetate, In Johnston JR (ed), Molecular Genetics of Yeast: A Practical Approach, Oxford University Press, New York, 121134 (1994)

DH5α and DH10B are trademarks of Life Technologies. QIAprep is a trademark of QIAGEN. Strataprep, XL1-Blue, XL2-Blue, and XL10-Gold are trademarks of Stratagene. Wizard Plus and SV Plus are trademarks of Promega.

back to top
'"/>

Source:

Page: All 1 2 3 4 5
Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications