Navigation Links
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

termine whether bacterial density affected the quality or quantity of plasmid DNA isolated, we tested the effect of using different ODml quantities of bacteria in Aurum minipreps. We found that plasmid yield increased until the maximum recommended load of bacterial culture (12 ODml) was reached (Table 4). In all cases, the plasmid yield was lower than was normally attained with cells grown in LB Amp medium, and we attribute this to using 2x YT medium. Using 50% or 100% more culture than recommended reduced the plasmid yield without greatly affecting the quality of the preps (Figure 2). We concluded that for Aurum minipreps, optimum quality and quantity of plasmid are obtained using 12 ODml of cells grown in LB Amp. Henceforth, LB Amp was used exclusively as a growth medium.

We were interested in testing how the new Aurum kits compared with other plasmid purification kits commonly used in our laboratory. We found that plasmid yields using the other kits were dramatically lower than what we achieved from similar cultures with the Aurum plasmid kits (Table 5). Aurum preps were of high quality, equivalent or better than those obtained from other kits (Figure 3, Tables 5 and 6).

We tested the quality of isolated plasmid DNA by four criteria: restriction digests, DNA sequencing, and bacterial or yeast transformations. All kits tested gave plasmid DNA that was digested equivalently by restriction enzymes (Figure 4). Two plasmid samples gave poor or unusual restriction digestion patterns (Figure 4, lanes 3 and 11), and both of these plasmids were isolated from endA+ bacterial strains. We noticed no differences in the ability of isolated plasmids to transform either bacteria or yeast.
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:9/15/2014)... of researchers led by Northeastern University has developed ... junctions and a variety of nanocarbon structures in ... easily scalable, which will allow the researchers to ... use in applications ranging from electronic devices to ... to sports equipment. , Their findings were published ...
(Date:9/15/2014)... Sept. 15, 2014 Research and Markets ... Market by Product, by Services, by Technology, by Application - ... Whole Exome Sequencing (WES) is next-generation technology used ... the exon. The exome, which is the total of all ... genome and is actually converted into proteins after translation. It ...
(Date:9/15/2014)... Maryland , 15 septembre 2014 ... Awareness Week (IPAW) (semaine internationale sur la ... 18 octobre. Initiative conjointe de la Plasma ... sociétés membres , l,IPAW vise à : ... au sujet de la collecte du plasma-aphérèse ...
(Date:9/14/2014)... MD (PRWEB) September 15, 2014 ... be held on Tuesday, October 21, 2014, from 12:00 ... overview of the program’s innovative curriculum and practice-oriented instruction, ... a graduate degree can help advance their career in ... RSVP online . , About UMBC’s Biotechnology ...
Breaking Biology Technology:Northeastern University Researchers Develop Novel Method for Working with Nanotubes 2Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 2Global Whole Exome Sequencing (Systems, Kits, Library Preparation, Target Enrichment) Market by Product, by Services, by Technology, by Application - Forecast to 2018 3L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 2L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 3UMBC Biotechnology Graduate Programs’ Virtual Info Session, October 21, 2014 2
... AMDL, Inc. (Amex:,ADL), headquartered in Tustin, California, ... through its wholly owned subsidiary Jade,Pharmaceutical Inc., ... with JPI, engages in the development, manufacture ... diagnostic products. The,Company announced today that JPI,s ...
... Jan. 30 Phosphagenics,Limited ("Phosphagenics") (ASX: POH, AIM: ... study demonstrating that the,combination of its APA- 01 ... serum lipids and inflammatory proteins,involved in the development ... of the study was to determine whether a ...
... SCHAUMBURG, Ill., Jan. 30 Sagent Pharmaceuticals, Inc.,a ... products, today announced that Ronald E. Pauli, chief ... Pharmaceutical,Biotechnology and Medical Device Conference on Tuesday, February ... be held February 5-7, 2008 at the Grand,Hyatt ...
Cached Biology Technology:AMDL Wins Chinese Military Supply Agreement for Its Domperidone Product for up to $6.6 Million 2Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 2Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 3Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 4
(Date:9/15/2014)... Australia,s leading coral reef ecologists fears that reef biodiversity ... survival that we once thought. , In an international ... Centre of Excellence for Coral Reef Studies (Coral CoE) ... important species within reef ecosystems. , In coral reefs, ... jobs that keep the ecosystem safe and functioning. ...
(Date:9/15/2014)... an assistant professor in the Department of Science and ... and Human Sciences at Virginia Tech, has won a ... to study the prospects and problems of creating a ... of Japan,s Fukushima I Nuclear Power Plant in March ... community viewed nuclear emergencies, Schmid said. Three of the ...
(Date:9/15/2014)... CAMBRIDGE, MA -- Speed and agility are hallmarks of the ... Earth, able to accelerate to 60 mph in just a ... cheetah pumps its legs in tandem, bounding until it reaches ... algorithm for bounding that they,ve successfully implemented in a robotic ... electric motors that weighs about as much as its feline ...
Breaking Biology News(10 mins):Specialized species critical for reefs 2'Nuclear disasters don't respect national boundaries' 2Run, cheetah, run 2Run, cheetah, run 3
... human walking and running motions and whether the hips, knees ... researchers at North Carolina State University show that the hips ... generate more of the power when humans run. Knees provide approximately ... The research could help inform the best ...
... Mongolia are a new species of birch mouse, indicating that ... reported, according to paleontologist Yuri Kimura at Southern Methodist University ... in sediments that are 17 million years old, said Kimura, ... primus to include the Latin word for "first." ...
... from Galicia in Spain have used forensic mitochondrial DNA ... mackerel. This method makes it possible to genetically differentiate ... processed, which makes it easier to monitor the degree ... from the mitochondria cell organelles is ideal ...
Cached Biology News:Hips take walking in stride; ankles put best foot forward in run 2Birch mouse ancestor discovered in Inner Mongolia is new species of rare 'living fossil' 2Birch mouse ancestor discovered in Inner Mongolia is new species of rare 'living fossil' 3Birch mouse ancestor discovered in Inner Mongolia is new species of rare 'living fossil' 4Sardines and horse mackerel identified using forensic techniques 2
Total Proteins from over 50 different human Alzheimer, Diabetic, Liver Cirrhosis, and Lupus diseased tissues are available....
...
Normal Hamster Serum...
... Thrust Slide is designed for heavy duty ... limited and substantial side-loading is present. Standard ... millions of trouble-free cycles even in high ... or recirculating ball bearings , Optional corrosion ...
Biology Products: