Navigation Links
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

termine whether bacterial density affected the quality or quantity of plasmid DNA isolated, we tested the effect of using different ODml quantities of bacteria in Aurum minipreps. We found that plasmid yield increased until the maximum recommended load of bacterial culture (12 ODml) was reached (Table 4). In all cases, the plasmid yield was lower than was normally attained with cells grown in LB Amp medium, and we attribute this to using 2x YT medium. Using 50% or 100% more culture than recommended reduced the plasmid yield without greatly affecting the quality of the preps (Figure 2). We concluded that for Aurum minipreps, optimum quality and quantity of plasmid are obtained using 12 ODml of cells grown in LB Amp. Henceforth, LB Amp was used exclusively as a growth medium.

We were interested in testing how the new Aurum kits compared with other plasmid purification kits commonly used in our laboratory. We found that plasmid yields using the other kits were dramatically lower than what we achieved from similar cultures with the Aurum plasmid kits (Table 5). Aurum preps were of high quality, equivalent or better than those obtained from other kits (Figure 3, Tables 5 and 6).

We tested the quality of isolated plasmid DNA by four criteria: restriction digests, DNA sequencing, and bacterial or yeast transformations. All kits tested gave plasmid DNA that was digested equivalently by restriction enzymes (Figure 4). Two plasmid samples gave poor or unusual restriction digestion patterns (Figure 4, lanes 3 and 11), and both of these plasmids were isolated from endA+ bacterial strains. We noticed no differences in the ability of isolated plasmids to transform either bacteria or yeast.
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:9/2/2014)... PA (PRWEB) September 02, 2014 ... a new highly efficient, cost effective approach to ... Patent No. 8,820,265 by the U.S. Patent and ... to Opertech. , “Opertech’s taste evaluation ... the first automated high throughput system for rapid ...
(Date:9/2/2014)... Md. , Sept. 2, 2014  Synthetic ... novel anti-infective biologic and drug candidates targeting specific ... today positive results from its final preclinical toxicology ... Administration (FDA) guidance, this bridging study was required ... candidate designed to prevent the devastating effects of ...
(Date:9/2/2014)... Massachusetts , September 2, 2014 ... to BioData,s research and lab management services  ... management service, is pleased to announce the appointment of ... Culot most recently served as VP Marketing and Business ... life science research and clinical applications. Previously, ...
(Date:9/1/2014)... September 01, 2014 The Collaborative ... Diagnostics report provides comprehensive understanding and unprecedented access ... into by the worlds leading life science companies. ... analysis of how and why companies enter collaborative ... prospective partner’s negotiated deals terms provides critical insight ...
Breaking Biology Technology:Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5Louis Culot Appointed as BioData's CEO 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 3Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 4
... Bioengineers in the United States have modified a strain ... The research, published in Biotechnology and Bioengineering, ... for strain improvement, a positive development for the biofuel ... mobilis, a bacterium noted for its bio-ethanol producing potential. ...
... Inc. today announced a collaboration with Syngenta Ventures ... low-cost sugars for a variety of industrial applications. ... to Agrivida access to crop technology and intellectual ... Agrivida will bolster its existing technology portfolio, ...
... 2010) -- Nanotechnologists, marine biologists and signal-processing experts from ... Mass., and other U.S. universities have won a $6 ... unlock the secrets of nature,s best camouflage artists. Ultimately, ... of the elegant skin colors and patterns produced by ...
Cached Biology Technology:Bioengineers develop bacterial strain to increase ethanol biofuel production 2Agrivida Announces Collaboration With Syngenta Ventures to Deliver Low-Cost Sugars for Cellulosic Biofuels and Biochemicals 2Nano squid skin: DOD awards $6M for metamaterials research 2Nano squid skin: DOD awards $6M for metamaterials research 3
(Date:9/2/2014)... Washington D.C., September 2, 2014 A study ... Journal of the American Academy of Child ... adolescents diagnosed with a psychiatric diagnosis had an ... exposed to antipsychotics. , Using data from the ... by Dr. Ren Ernst Nielsen, Psychiatry, Aalborg University ...
(Date:9/2/2014)... DURHAM, N.C. -- When you accidentally touch a hot ... know the basic neural circuits involved in sensing and ... out the molecular players. , Duke researchers have made ... molecule involved in pain in worms, and have built ... Sept. 2 in Nature Communications , may help ...
(Date:9/1/2014)... that corals whose calcium-carbonate skeletons form the ... that rely entirely on ocean currents to deliver ... now scientists at MIT and the Weizmann Institute ... they are far from passive, engineering their environment ... enhance their ability to exchange nutrients and dissolved ...
Breaking Biology News(10 mins):Risk of diabetes in children and adolescents exposed to antipsychotics 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3Nature's tiny engineers 2Nature's tiny engineers 3
... been prescribed by traditional healers in Brazil to treat a range ... , Now for the first time, researchers at Newcastle University have ... Hyptis crenata otherwise known as Brazilian mint. , Testing ... researcher Graciela Rocha was able to show that when prepared as ...
... Scientists have crystallised a protein that may help gut bacteria ... probiotic producers to identify strains that are likely to be of ... lining the gut to have a beneficial effect, and if they ... stick around long enough to exert their activity," says Dr Nathalie ...
... American College of Medical Toxicology (ACMT) have signed an agreement ... Medical Toxicology (JMT), the official journal of the ACMT, ... the University of Pennsylvania Press. The Journal ... to advancing the science and practice of medical toxicology. ...
Cached Biology News:Got a pain? -- Have a cup of Brazilian mint 2A sticky solution for identifying effective probiotics 2Springer and the American College of Medical Toxicology to work together 2
... Contents include: Microarray hybridization chamber assembly ... top, clamp, thumbscrew) Microarray chamber user guide ... are intended to be cleaned and reused. ... Agilents Catalog Oligo Microarray kits and all ...
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells....
... PEP-126 is an 18 amino acid synthetic peptide whose sequence ... of this peptide is (amino to carboxy terminus): M(1) - A ... P - A - S - P - R - D ... This peptide may be used for neutralization and control experiments ...
Rabbit polyclonal antibody to Glutathione-S-Transferase (GST)...
Biology Products: