Navigation Links
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

oximately 8 x 108 cells/ml


Results
A wide variety of E. coli strains are frequently used for the propagation of plasmids. We have found that certain bacterial strains and certain plasmid purification kits can result in poor plasmid yield or poor plasmid quality. Therefore, we assessed the yield and quality of plasmids isolated from 14 common laboratory E. coli strains with the new Aurum miniprep and midiprep kits. We tested both endA- and endA+ bacterial strains because both the quality and quantity of plasmid DNA isolated from endA+ strains can be poor. The yields from these experiments are summarized in Tables 1 and 2. On average, ~20 g and 120 g of plasmid were isolated from minipreps and midipreps, respectively. The yields from minipreps varied more than from midipreps, but this may be because more variables (strains, endA- vs. endA+) were tested. In general, yield correlated most closely to specific plasmid type. Notably, good yields of plasmid could be obtained from endA+ strains.

Bacterial growth media can affect the quality and quantity of isolated plasmid DNA. At least four different media are commonly used for the growth of E. coli. We routinely use LB Amp but were interested in comparing the plasmid yield and quality when LBG Amp, 2x YT, or TB Amp was used. We found that plasmid yield seems to vary considerably with the type of medium used (Table 3). Notably, LBG Amp medium gave poor yields and plasmids that migrated unusually on agarose gels (Table 3, Figure 1). LB Amp and TB Amp gave the best yields and highest quality of plasmid.

To de
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:10/22/2014)... BOSTON , Oct. 22, 2014  Actavis ... specialty pharmaceutical company, and Rhythm, a biopharmaceutical company, today ... to acquire Rhythm,s wholly owned subsidiary, Rhythm Health, Inc., ... for the treatment of diabetic gastroparesis and other GI ... successful Phase 2 trial of relamorelin for the treatment ...
(Date:10/22/2014)... WriteResult, LLC – a premier provider ... with a team of researchers from Yale University’s School ... Collaborative to provide electronic questionnaire data collection through use ... started in May, aims to evaluate the impact of ... to and participation in urban agriculture to impact their ...
(Date:10/22/2014)... ROCKVILLE, Md. , Oct. 22, 2014   ... company developing novel pathogen-specific therapies for serious infections and ... Office has issued a Notice of Allowance for a ... product in its C. difficile program, SYN-004. This ... to SYN-004 in the U.S. and adds to the ...
(Date:10/20/2014)... 20, 2014 The report “Pharmacy ... Systems, Packaging and Labeling Systems, Table-top Counters) by ... Global Forecasts & Trends to 2019” analyzes and ... in North America, Europe, Asia-Pacific, and the Rest ... tables and 30 figures spread through 300 pages ...
Breaking Biology Technology:Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 2Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 3Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 4Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 5myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 4Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4
... CHESTER, Pa., April 1, 2008 VWR International,LLC, ... that it,has acquired Jencons (Scientific) Limited, a UK ... the UK,s largest suppliers of laboratory equipment,and consumables ... company also has,operations in Ireland, Kenya and North ...
... and MONTREAL, April 1 /PRNewswire-FirstCall/ - AEterna,Zentaris ... a global biopharmaceutical company,focused on endocrine therapy ... a leading Canadian specialty pharmaceutical company, announced ... the transactions under their,previously announced purchase and ...
... 1, 2008 Piedmont Heart Institute (PHI),expands its ... practice, Cardiology of Georgia, P.C. (COG), through a ... With eight,locations including Buckhead, Canton, Fayetteville and North ... the Piedmont Heart Institute and an,important addition to ...
Cached Biology Technology:VWR International, LLC Acquires Jencons (Scientific) Ltd, UK 2AEterna Zentaris and Paladin Labs Complete Sale and Purchase of Miltefosine Rights 2AEterna Zentaris and Paladin Labs Complete Sale and Purchase of Miltefosine Rights 3Piedmont Heart Institute Welcomes Cardiology of Georgia 2Piedmont Heart Institute Welcomes Cardiology of Georgia 3Piedmont Heart Institute Welcomes Cardiology of Georgia 4
(Date:10/15/2014)... to assess the pandemic risk from strains of influenza ... not allow ourselves to become complacent that the most ... of scientists. , Influenza pandemics arise when a new ... develop widespread immunity – spreads in the human population. ... 100 years, the worst of which – the 1918 ...
(Date:10/15/2014)... Gutenberg University Mainz (JGU) as a full member, ... maintain a significant nationwide standing in the field ... Johannes Gutenberg University Mainz as a full member ... national and international competition for the best minds ... be proud that Mainz University has been recognized ...
(Date:10/15/2014)... have linked increased resistance to bacterial pneumonia in female ... hormone estrogen. , Females are naturally more resistant to ... scientists has shown that increased resistance to bacterial pneumonia ... oxide synthase 3 (NOS3). They also show that this ... female sex hormone estrogen. , The team, lead by ...
Breaking Biology News(10 mins):Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3
... seen the story in the news before. Whether it,s the death ... in August, or of an elderly woman gardening in the middle ... condition for which there is no treatment beyond submersion in ice ... to a normal temperature. But, in a new ...
... of what living cells do is carried out by "molecular ... carry out some biological function. How the minute steps of ... a favorite target for creationists. In a study published ... a team of scientists from the University of Chicago and ...
... warmest since global climate has been measured, and while ... plant communities that reflect this warming trend, no study ... until now. With the publication of "Continent-wide ... Advance Online Publication (AOP) in Nature Climate Change ...
Cached Biology News:'Couch potato pill' might stop heat stroke too 2'Couch potato pill' might stop heat stroke too 3Evolution of complexity recreated using 'molecular time travel' 2Evolution of complexity recreated using 'molecular time travel' 3Evolution of complexity recreated using 'molecular time travel' 4European mountain vegetation shows effects of warmer climate 2