Navigation Links
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Audra Day, Colette Schneider, Lisa Ottmers, and Brandt L. Schneider Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.


Introduction
Small-scale isolation and purification of plasmid DNA from E. coli bacterial strains is a widely used and essential technique in molecular biology laboratories. With the rapid pace of molecular research, it is vitally important that plasmid DNA isolation and purification protocols and commercially available kits satisfy four criteria they should be reliable, efficient, reproducible, and cost-effective. We conducted an extensive test of Bio-Rads new Aurum miniprep and midiprep plasmid DNA isolation kits. We conclude that these kits satisfy all of the above criteria.


Methods
Bacterial strains were transformed with 1 l of 10 pg/l of various plasmids by electroporation (Bio-Rad Gene Pulser electroporator), and plated to LB plates containing 100 g/ml ampicillin (Amp). Single colonies were grown to saturation overnight in 3 ml of liquid LB containing 100 g/ml Amp for minipreps, or 30 ml of the same medium for midipreps. Densities of the cultures were determined according to the Aurum protocol, and 12 ODml* or 50 ODml of each culture were used for plasmid purification for miniprep and midipreps, respectively. Plasmid concentrations were quantitated with Bio-Rads VersaFluor fluorometer and Hoechst dye 33258 DNA quantitation kit. Plasmid quality was assessed by four criteria: restriction digests, transformation efficiency with both bacterial and yeast hosts, and sequencing. Restriction digests were done on 500 ng of plasmid in 20 l with EcoRI (NEB)
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Mx4000 Multiplex Quantitative PCR System*,PPP
2. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
3. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
4. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
5. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
6. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
7. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
10. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
11. Quantitative Analysis of the Cell Cycle in Imaging Applications
Post Your Comments:
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, Inc., ... the relaunch of his highly informative blog, now named Stem ... What are Stem Cells ?” Dr. Harman’s purpose for ... the basics of stem cell therapy so that pet owners ... treatment when considering regenerative medicine. , A veterinarian by trade, ...
(Date:7/10/2014)... Senior supply chain management executives from over 50 ... of “Reducing Cost, Lead Time, & Defects in the ... the partnership of the Bio Supply Management Alliance ( ... SCMI) of the University of San Diego in ... Institute of Peace and Justice in San Diego, Devendra ...
(Date:7/10/2014)... , July 10, 2014  Kainos Capital, a firm ... today announced that it has acquired the Slim-Fast brand ... the business. Terms of the transaction were not disclosed. ... meal replacement business that markets ready-to-drink shakes, powders, bars ... America and in the United ...
(Date:7/10/2014)... Calif., July 10, 2014   Ceres, Inc . ... seed company, announced today financial results for the three ... on its business. Ceres reported that the ... product performance this growing season in Brazil, which concluded ... the company,s sorghum evaluation areas for part of the ...
Breaking Biology Technology:Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4Kainos Capital Acquires Slim-Fast From Unilever 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... Inc. (Nasdaq: PPHM ) today announced that Thomas A. ... on Wednesday. Dr. Waltz had been a director of Peregrine ... of his death, Dr. Waltz served on Peregrine,s Audit Committee, Compensation ... "The sudden loss of our business colleague and friend ...
... - SCRIP Awards Recognises the Best and Brightest in ... - Glenmark is the Only Indian Company to ... The best and brightest of ... London at the 2008 SCRIP Awards hosted,by the leading UK broadcaster ...
... LASIK and refractive eye surgery experts listed in ... at the recently concluded American Academy of Ophthalmology ... Epstein, Dr. Walter Stark, Dr. James McCulley, Dr. ... Stahl, and Dr. Bruce Koffler, all leading refractive ...
Cached Biology Technology:Peregrine Pharmaceuticals Mourns Loss of Chairman Thomas A. Waltz 2SCRIP, the Leading Pharmaceutical Magazine in the World Crowns Glenmark as the "Best Pharma Company in the World - SME" and the "Best Company in Emerging Markets" at the SCRIP Awards 2008 in London 2Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 2Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 3Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 4Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 5Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 6Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 7Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 8Top LASIK and Refractive Experts in Trusted LASIK Surgeons Directory Present Research and Educate Their Peers at Annual AAO Meeting in Atlanta 9
(Date:7/11/2014)... Janeiro, Brazil- In the brains of all vertebrates, ... allows an electric or chemical signal to be ... synapses, which are the most abundant type of ... formation is crucial for learning, memory, perception and ... synapses critical for brain function. For instance, ...
(Date:7/10/2014)... Mass. , June 30, 2014  Aware, Inc. ... biometrics software and services, previously announced on June 26, ... special cash dividend of $1.75 per share, or approximately ... date of July 10, 2014 and a payment date ... by NASDAQ that it had set an ex-dividend date ...
(Date:7/10/2014)... , July 2, 2014 NXT-ID, Inc., (OTCQB: NXTD) a biometric ... CEO Mr. Gino Pereira was interviewed on July ... Tampa Florida . Mr. Pereira discusses the ... Cedric Harris and how the Wocket™ aims to replace ... Cedric Harris tells Gino he has ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2NASDAQ Sets Ex-Dividend Date for Aware, Inc.'s Special Cash Dividend of $1.75 Per Share 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4
... release is available in German . , ... 200 times lighter than glass typically used for PV, ... vs. rigid glass CdTe systems. High-speed and low-cost roll-to-roll ... flexible solar cells on polymer film as substrates. The ...
... adding gravel to a river to replace lost sediments won,t ... water refuges that protect fish from thermal pollution, according to ... The researchfeatured in the June 2011 issue of Science ... to explore the interplay between sub-surface water flow and temperature ...
... often viewed as individuals that compete with one another for ... environments actually benefit from positive, facultative interactions? The authors ... for certain tree speciesand that it may take the form ... been observed in over 150 species of plants around the ...
Cached Biology News:Efficiency record for flexible CdTe solar cell due to novel polyimide film 2'Thermal pollution' in rivers not fully mediated by gravel augmentation 2Is root grafting a positive, cooperative behavior in trees? 2Is root grafting a positive, cooperative behavior in trees? 3
ANTI MYCOBACTERIUM TUBERCULOSIS...
IMAGEQUANT 100 IQTL, 1 EA. Category: ImageQuant CCD Imagers....
ImageQuant 100 UV, 1 EA. Category: ImageQuant CCD Imagers....
The FlowTACS Apoptosis Detection Kit is designed specifically for in situdetection of apoptotic...
Biology Products: