QIAshredder spin columns are designed for simple and rapid homogenization
of cell and tissue lysates.Format:
Mini spin columns
Animal or human cell or tissue lysates,
plant cell or tissue lysates, white blood cell lysates
Up to 700 l cell lysate
Accessory for use with the RNeasy Mini Kit and Oligotex
Direct mRNA Kits
Features and benefitsComparison of RNA Yields
with Different Homogenization Methods
Replaces syringe-and-needle homogenization
Reduces loss of sample material
Eliminates cross-contamination between samples
Filters out insoluble debris and reduces viscosity
Northern blot of total RNA isolated from 5 x 106 HeLa cells using
the RNeasy Mini procedure with the indicated homogenization methods.
RNA was eluted with 2 x 40 l water, and 10 l was loaded
per lane. QIAshredder Kit options
QIAshredder Kits are available with 50 or 250 QIAshredder
homogenizers for use with the RNeasy Mini Kit and Oligotex Direct
mRNA Kits. QIAshredder spin columns are also in
cluded in the:
RNeasy Plant Mini Kit
DNeasy Plant Mini Kit
QIAamp RNA Blood Mini Kit
The QIAshredder unit consists of a unique biopolymer shredding system
in a microcentrifuge spin-column format. Isolation of total or poly A+ RNA
from cell or tissue lysates requires homogenization to reduce viscosity
caused by high-molecular-weight cellular components and cell debris. Traditional
methods use syringes and needles, which are tedious, inconvenient, and hazardous
to handle. QIAshredder spin columns replace these homogenization methods
with a fast and simple centrifugation step. In general, similar yields and
RNA quality are obtained for cell lysates as with rotorstator homogenization
(see figure "Comparison of RNA Yields with Different
Up to 700 l of a cell lysate can be homogenized by pipetting or
decanting the lysate into a QIAshredder unit and centrifuging for 2 minutes
at full speed in a microcentrifuge. The homogenized lysate is ready to use
in nucleic acid isolation procedures.
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