For cleanup of DNA from PCR or other enzymatic
reactions
Application:
PCR cleanup
Format:
Spin columns
Throughput:
124 samples
DNA size range:
100 bp 10 kb
Equipment required:
Microcentrifuge or vacuum manifold
Features and benefits
Purified ds or ssDNA in 5 minutes from PCR amplification
9095% recovery of fragments (100 bp 10 kb)
>99.5% primer removal
Separation of >100 bp product from 40mer primer
No removal of mineral oil necessary
Elution in as little as 30 l Tris buffer or water
Complete Primer Removal after PCR
Analysis of PCR products before (b) and after (a) purification
with the QIAquick PCR Purification Kit. Samples were analyzed
on a 1% TAE agarose gel. M: markers.