Navigation Links
QIAfilter Plasmid Kits

For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA

Features and benefits
  • Up to 10 mg of ultrapure plasmid DNA
  • Cleared lysates from up to 2.5 liters of LB culture without centrifugation
  • Reduced plasmid purification time
  • Fast, easy, and convenient handling for small to large culture volumes


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.
For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

QIAfilter principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Midi and Maxi Cartridges have a syringe-format (see figure "QIAfilter Midi Cartridge") and lysates are cleared in a matter of seconds by pushing the liquid through the filter. QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort (see figure "QIAfilter Mega-Giga Cartridge").

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is then applied directly to a QIAGEN-tip for plasmid DNA purification.

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection and automated fluorescent sequencing, including capillary sequencing.

QIAfilter Midi Cartridge
QIAfilter Mega-Giga Cartridge The syringe-format QIAfilter Midi Cartridge in use. The vacuum-operated QIAfilter Mega- Giga Cartridge in use.
Please note that the bottle is not included in the kits.




'"/>

Source:


Page: All 1 2

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
6. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Basic Plasmid DNA Isolation Protocol
9. RNA-free Plasmid DNA Isolation Protocol
10. Basic Plasmid DNA Isolation Protocol
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/27/2016)... and READING, England , ... Indegene ( http://www.indegene.com ), ein führender Anbieter ... die Life-Science-Branche, Pharmaunternehmen und Gesundheitsorganisationen, und TranScrip ... von innovativen wissenschaftlichen Support-Services für den gesamten ... IntraScience heute den Ausbau ihrer bestehenden Allianz ...
(Date:5/26/2016)... 26, 2016  Agriculture nutrients are in the news ... is running their nitrate removal system on ... and coastal regions nationwide are painting the ... widespread issue. NECi Superior Enzymes, a biotechnology ... developed a new, easy to use device that fits ...
(Date:5/26/2016)... Q BioMed Inc. (OTCQB: QBIO), a biotechnology acceleration ... at the 5th Annual Marcum MicroCap Conference on Thursday, June ... at the Grand Hyatt Hotel. The Company,s presentation ... scheduled to begin at 11a.m ET in the Broadway Room. ... and outline milestones for the balance of 2016 and beyond. ...
(Date:5/26/2016)... ... May 26, 2016 , ... After several promising treatments in ... the City of Knowledge in Panama, a 6 year-old Duchenne’s muscular dystrophy patient ... this year following FDA approval of a second application for a single patient, ...
Breaking Biology Technology:
(Date:3/21/2016)... March 22, 2016 Unique ... passcodes for superior security   ... provider of secure digital communications services, today announced it ... and offer enterprise customers, particularly those in the Financial ... and voice authentication within a mobile app, alongside, and ...
(Date:3/17/2016)... ABI Research, the leader in transformative ... market will reach more than $30 billion by ... Consumer electronics, particularly smartphones, continue to boost the ... reach two billion shipments by 2021 at a ... Research Analyst at ABI Research. "Surveillance is also ...
(Date:3/15/2016)... 15, 2016 Yissum Research Development Company ... company of the Hebrew University, announced today the formation ... technology of various human biological indicators. Neteera Technologies has ... from private investors. ... detection of electromagnetic emissions from sweat ducts, enables reliable ...
Breaking Biology News(10 mins):