Navigation Links
QIAfilter Plasmid Kits

For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA

Features and benefits
  • Up to 10 mg of ultrapure plasmid DNA
  • Cleared lysates from up to 2.5 liters of LB culture without centrifugation
  • Reduced plasmid purification time
  • Fast, easy, and convenient handling for small to large culture volumes


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.
For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

QIAfilter principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Midi and Maxi Cartridges have a syringe-format (see figure "QIAfilter Midi Cartridge") and lysates are cleared in a matter of seconds by pushing the liquid through the filter. QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort (see figure "QIAfilter Mega-Giga Cartridge").

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is then applied directly to a QIAGEN-tip for plasmid DNA purification.

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection and automated fluorescent sequencing, including capillary sequencing.

QIAfilter Midi Cartridge
QIAfilter Mega-Giga Cartridge The syringe-format QIAfilter Midi Cartridge in use. The vacuum-operated QIAfilter Mega- Giga Cartridge in use.
Please note that the bottle is not included in the kits.




'"/>

Source:


Page: All 1 2

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
6. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Basic Plasmid DNA Isolation Protocol
9. RNA-free Plasmid DNA Isolation Protocol
10. Basic Plasmid DNA Isolation Protocol
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/21/2017)... ... February 21, 2017 , ... The medical potential of stem cells is both ... of medicine, due to their differentiating characteristics. Stem cells are unique as the have ... be induced to become tissue or organic-specific cells with special functions. , Stem ...
(Date:2/21/2017)... , ... February 21, 2017 , ... ... cell therapy manufacturing facility at its headquarters laboratory in Poway, California. Based ... the experience of both in-house personnel and consultants, VetStem constructed and validated a ...
(Date:2/21/2017)... and VANCOUVER, British Columbia , ... OGXI ) today announced that apatorsen results from two randomized ... of Clinical Oncology (ASCO) 2017 Genitourinary Cancers Symposium, held February ... . Clinical data from trials in bladder and prostate ... administered in combination with standard-of-care treatments. ...
(Date:2/21/2017)... --  Logicalis Healthcare Solutions , the healthcare-focused practice within ... provider ( www.us.logicalis.com ), today announced a new service ... service will help hospital CIOs make the best use ... and other clinicians immediate access to a pool of ... hospital IT staff to focus on broader issues such ...
Breaking Biology Technology:
(Date:2/13/2017)... , Feb. 13, 2017  RSA Conference -- RSA, ... that is designed to enhance fraud detection and ... in the RSA Fraud & Risk Intelligence Suite. ... to leverage additional insights from internal and external ... better protect their customers from targeted cybercrime attacks. ...
(Date:2/8/2017)... 2017 About Voice Recognition Biometrics Voice recognition ... against a stored voiceprint template. Acoustic features of ... tone are compared to distinguish between individual voices. ... most PCs already have a microphone and can ... biometrics are most likely to be deployed in ...
(Date:2/8/2017)... , Feb. 7, 2017 Report Highlights ... billion by 2021 from $8.3 billion in 2016 at ... 2016 to 2021. Report Includes - An overview ... global market trends, with data from 2015 and 2016, ... 2021. - Segmentation of the market on the basis ...
Breaking Biology News(10 mins):