For fast purification of up to 10 mg ultrapure
plasmid or cosmid DNA
Features and benefits
- Up to 10 mg of ultrapure plasmid DNA
- Cleared lysates from up to 2.5 liters of LB culture without centrifugation
- Reduced plasmid purification time
- Fast, easy, and convenient handling for small to large culture volumes
* Actual yields depend on plasmid copy number, size of insert, host strain,
culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host
strain, and culture medium.
For purification of low-copy plasmids and cosmids, QIAfilter Plasmid
Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the
large culture volumes required and the limited capacity of the QIAfilter
QIAfilter Cartridges, provided in QIAfilter, HiSpeed
and EndoFree Plasmid
, are special filter units designed to replace centrifugation following
alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove
SDS precipitates and clear bacterial lysates in a fraction of the time needed
for centrifugation, reducing plasmid-purification time by up to 1 hour.
QIAfilter Midi and Maxi Cartridges have a syringe-format (see figure
"QIAfilter Midi Cartridge") and lysates
are cleared in a matter of seconds by pushing the liquid through the filter.
QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently
clear even large volumes of bacterial lysate with minimal effort (see
figure "QIAfilter Mega-Giga Cartridge").
Neutralized bacterial lysates are incubated directly in the QIAfilter
Cartridge and cleared in seconds by filtration. The filtrate is then applied
directly to a QIAGEN-tip for plasmid DNA purification.
QIAfilter Midi Cartridge
QIAfilter Mega-Giga Cartridge
DNA obtained with QIAfilter Plasmid Kits provides excellent results in
all applications, from cloning to transfection and automated fluorescent
sequencing, including capillary sequencing.
The syringe-format QIAfilter Midi Cartridge in use.
The vacuum-operated QIAfilter Mega- Giga Cartridge in use.
Please note that the bottle is not included in the kits.
Source:Page: All 1 2 Related biology technology :1
. Efficiently Insert Unique Restriction Sites into Plasmid Vectors2
. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors3
. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications
and Agarose Gels4
. Generate High-Quality, Directional Plasmid cDNA Libraries5
. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction
. New Fusion Trans-Activator Plasmids for Studying Signal Transduction
. Comparison of Growth Techniques and Media for the Purpose of Plasmid
Isolation from E. coli Using the Eppendorf
Perfectprep Plasmid Mini
. Basic Plasmid DNA Isolation Protocol9
. RNA-free Plasmid DNA Isolation Protocol10
. Basic Plasmid DNA Isolation Protocol11
. RNA-free Plasmid DNA Isolation Protocol