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QIAfilter Plasmid Kits

For fast purification of up to 10 mg ultrapure plasmid or cosmid DNA

Features and benefits
  • Up to 10 mg of ultrapure plasmid DNA
  • Cleared lysates from up to 2.5 liters of LB culture without centrifugation
  • Reduced plasmid purification time
  • Fast, easy, and convenient handling for small to large culture volumes


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.
For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

QIAfilter principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Midi and Maxi Cartridges have a syringe-format (see figure "QIAfilter Midi Cartridge") and lysates are cleared in a matter of seconds by pushing the liquid through the filter. QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort (see figure "QIAfilter Mega-Giga Cartridge").

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is then applied directly to a QIAGEN-tip for plasmid DNA purification.

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection and automated fluorescent sequencing, including capillary sequencing.

QIAfilter Midi Cartridge
QIAfilter Mega-Giga Cartridge The syringe-format QIAfilter Midi Cartridge in use. The vacuum-operated QIAfilter Mega- Giga Cartridge in use.
Please note that the bottle is not included in the kits.




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