Navigation Links
QIAamp DNA Blood BioRobot MDx Kit

For automated purification of genomic and mitochondrial DNA from whole blood*

Format: 96-well plates (for 3296 samples in increments of 8) QIAamp DNA Blood MDX Protocol Sample sources: Fresh or frozen whole blood (with common anticoagulants such as citrate and EDTA) Sample volume: 200 l Preparation time: 2.5 hours (including load check of 20 minutes) Typical yield: 36 g per 200 l healthy whole blood Elution volume: 200 l The QIAamp DNA Blood BioRobot MDx Kit provides automated, high-throughput DNA purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. The resulting DNA performs well in downstream amplification and Southern blotting assays (see figures "No PCR Inhibition" and "Reliable Real-Time PCR"). Advantages of the system include high process reliability and walkaway automation, providing a highly efficient DNA purification system for clinical laboratories.

No PCR Inhibition



A 1.2 kb fragment of the single-copy Hugl gene was amplified from DNA purified with the QIAamp DNA Blood BioRobot MDx Kit, using HotStarTaq DNA Polymerase in a 50 l reaction volume. No inhibition was observed using either A 1 l of eluate or B 20 l of eluate as template. (Data generated by QIAGEN R&D.)

Reliable Real-Time PCR



The QIAamp DNA Blood BioRobot MDx Kit was used to purify genomic DNA from 96 whole blood samples (each 200 l) collected from a single donor. The -actin gene was amplified from the purified DNA using the TaqMan system. An amplification plot of 96 PCR reactions is shown. (Data generated by QIAGEN R&D.)

* QIAamp Kits are intended as general-purpose reagents. No claim or representation is intended for their use to identify any specific organism or for clinical use (diagnostic, prognostic, therapeutic, or blood banking). It is the users responsibility to validate the performance of QIAamp Kits for any particular use, since the performance characteristics of these kits have not been validated for any specific organism.
Wash buffers are labeled with bar codes and expiration date as stated on the Q-card in the kit.

'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
3. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
4. EppendorfPerfect gDNA Blood Mini Kit
5. Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
8. Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume
9. EppendorfPerfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/20/2017)... ... June 20, 2017 , ... National ... well-versed leader with extensive assay development and biomarker expertise, as VP of Scientific ... Boston CRO specializing in bio-analytical assay development and sample testing services. The organization ...
(Date:6/20/2017)... , ... June 20, 2017 , ... ... discovery of antibody therapeutics from millions-diverse immune repertoires, announces launch of its new ... Diego, California. Dave Johnson, PhD, CEO of GigaGen, will present on Surge at ...
(Date:6/19/2017)... ... 19, 2017 , ... EDETEK, Inc., a clinical technology company ... launching two new additions of its award-winning cloud-based platform CONFORM™: Information Hub and ... 2017 Annual Meeting in Chicago, IL, June 19-22, 2017. , “Modern clinical trials ...
(Date:6/19/2017)... ... ... A colony of healthy honey bees is like a superorganism--individual bees provide ... containing nutrients necessary for growth and survival. Better nutrition gives the colony a strong ... a decline in honey bee health. Sick and weakened bees diminish the colony's resiliency, ...
Breaking Biology Technology:
(Date:4/5/2017)... -- KEY FINDINGS The global market for ... of 25.76% during the forecast period of 2017-2025. The ... the growth of the stem cell market. ... INSIGHTS The global stem cell market is segmented on ... stem cell market of the product is segmented into ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
(Date:3/30/2017)... 2017 The research team of The Hong ... fingerprint identification by adopting ground breaking 3D fingerprint minutiae recovery and ... speed and accuracy for use in identification, crime investigation, immigration control, ... ... A research team led ...
Breaking Biology News(10 mins):