For automated purification of genomic and mitochondrial
DNA from whole blood*
96-well plates (for 3296 samples in increments
QIAamp DNA Blood MDX Protocol
Fresh or frozen whole blood (with common anticoagulants
such as citrate and EDTA)
2.5 hours (including load check of 20 minutes)
36 g per 200 l healthy whole blood
The QIAamp DNA Blood BioRobot MDx Kit provides automated,
high-throughput DNA purification on the BioRobot
MDx using proven QIAamp technology. The fully automated procedure,
including bar code reading and complete process documentation, requires
less than 2.5 hours to process 96 samples (including a detailed load
check of 20 minutes) with no hands-on time during the run. The resulting
DNA performs well in downstream amplification and Southern blotting
assays (see figures "No PCR Inhibition"
and "Reliable Real-Time PCR"). Advantages
of the system include high process reliability and walkaway automation,
providing a highly efficient DNA purification system for clinical
No PCR Inhibition
A 1.2 kb fragment of the single-copy Hugl gene was amplified from
DNA purified with the QIAamp DNA Blood BioRobot MDx Kit, using HotStarTaq
DNA Polymerase in a 50 l reaction volume. No inhibition was
observed using either A 1 l of eluate or B 20
l of eluate as template. (Data generated by QIAGEN R&D.)
Reliable Real-Time PCR
The QIAamp DNA Blood BioRobot MDx Kit was used to purify genomic DNA
from 96 whole blood samples (each 200 l) collected from a single
donor. The -actin gene was amplified from the purified DNA
using the TaqMan system. An amplification plot of 96 PCR reactions
is shown. (Data generated by QIAGEN R&D.)
* QIAamp Kits are intended as general-purpose
reagents. No claim or representation is intended for their use to
identify any specific organism or for clinical use (diagnostic, prognostic,
therapeutic, or blood banking). It is the users responsibility
to validate the performance of QIAamp Kits for any particular use,
since the performance characteristics of these kits have not been
validated for any specific organism.
Wash buffers are labeled with bar codes and expiration date
as stated on the Q-card in the kit.
(Date:6/23/2016)... , June 23, 2016 Houston Methodist ... the Cy-Fair Sports Association to serve as their ... agreement, Houston Methodist Willowbrook will provide sponsorship support, ... connectivity with association coaches, volunteers, athletes and families. ... the Cy-Fair Sports Association and to bring Houston ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... today announced the launch of the Supplyframe Design Lab . Located in ... to explore the future of how hardware projects are designed, built and brought ...
(Date:6/23/2016)... Andrew D Zelenetz ... Published recently in Oncology ... touchONCOLOGY, Andrew D Zelenetz , discusses the ... is placing an increasing burden on healthcare systems ... With the patents on many biologics expiring, interest ...
(Date:6/23/2016)... , June 22, 2016 Amgen (NASDAQ: ... the QB3@953 life sciences incubator to accelerate ... The shared laboratory space at QB3@953 was created to ... key obstacle for many early stage organizations - access ... the sponsorship, Amgen launched two "Amgen Golden Ticket" awards, ...
(Date:6/22/2016)... 2016 On Monday, the Department of Homeland ... share solutions for the Biometric Exit Program. The Request ... Protection (CBP), explains that CBP intends to add biometrics ... the United States , in order to deter ... Logo - http://photos.prnewswire.com/prnh/20160622/382209LOGO ...
(Date:6/16/2016)... 16, 2016 The global ... to reach USD 1.83 billion by 2024, according ... Inc. Technological proliferation and increasing demand in commercial ... to drive the market growth. ... The development of advanced multimodal techniques for biometric ...
(Date:6/9/2016)... 9, 2016 Paris Police ... video security solution to ensure the safety of people and ... during the major tournament Teleste, an international technology ... services, announced today that its video security solution will be ... back up public safety across the country. The system roll-out ...