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QIAamp DNA Blood BioRobot MDx Kit

For automated purification of genomic and mitochondrial DNA from whole blood*

Format: 96-well plates (for 3296 samples in increments of 8) QIAamp DNA Blood MDX Protocol Sample sources: Fresh or frozen whole blood (with common anticoagulants such as citrate and EDTA) Sample volume: 200 l Preparation time: 2.5 hours (including load check of 20 minutes) Typical yield: 36 g per 200 l healthy whole blood Elution volume: 200 l The QIAamp DNA Blood BioRobot MDx Kit provides automated, high-throughput DNA purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. The resulting DNA performs well in downstream amplification and Southern blotting assays (see figures "No PCR Inhibition" and "Reliable Real-Time PCR"). Advantages of the system include high process reliability and walkaway automation, providing a highly efficient DNA purification system for clinical laboratories.

No PCR Inhibition



A 1.2 kb fragment of the single-copy Hugl gene was amplified from DNA purified with the QIAamp DNA Blood BioRobot MDx Kit, using HotStarTaq DNA Polymerase in a 50 l reaction volume. No inhibition was observed using either A 1 l of eluate or B 20 l of eluate as template. (Data generated by QIAGEN R&D.)

Reliable Real-Time PCR



The QIAamp DNA Blood BioRobot MDx Kit was used to purify genomic DNA from 96 whole blood samples (each 200 l) collected from a single donor. The -actin gene was amplified from the purified DNA using the TaqMan system. An amplification plot of 96 PCR reactions is shown. (Data generated by QIAGEN R&D.)

* QIAamp Kits are intended as general-purpose reagents. No claim or representation is intended for their use to identify any specific organism or for clinical use (diagnostic, prognostic, therapeutic, or blood banking). It is the users responsibility to validate the performance of QIAamp Kits for any particular use, since the performance characteristics of these kits have not been validated for any specific organism.
Wash buffers are labeled with bar codes and expiration date as stated on the Q-card in the kit.

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