For purification of up to 10 mg ultrapure plasmid
or cosmid DNA
Classic QIAGEN Plasmid Kits use gravity-flow anion-exchange tips for efficient
purification of plasmid DNA
Features and benefits
- Purity equivalent to that obtained by 2x CsCl-gradient centrifugation
- Reproducible yields of ultrapure DNA
- No ethidium bromide, no phenol, no chloroform, no CsCl
- Cost effective mini,* midi, maxi, mega, and giga preparations
* Note: For other miniprep spin and vacuum formats, See Plasmid
Miniprep Kit Selection guide
Actual yields depend on plasmid copy number, size of insert, host
strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host
strain, and culture medium.
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation
and the cleared lysate is applied to the QIAGEN-tip for plasmid DNA purification.
Eluted ultrapure DNA is recovered by isopropanol precipitation and centrifugation.
Plasmid DNA purified with QIAGEN Plasmid Kits is ideal for use in applications
such as transfection cloning, manual or automated sequencing, including
capillary sequencing, and in vitro transcription.
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