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QIAGEN Multiplex PCR Kit

AGEN Protocol")
  • Typing and detection of bacteria and viruses
  • Amplification of multiple DNA regions for SNP analysis Genotyping Transgenic Mice

    Transgenic mice were screened using the QIAGEN Multiplex PCR Kit and sets of 3 primers to distinguish wild-type (wt), heterozygous mutant (ht), and homozygous mutant (hm) mice. A Using a primer set for the recombination activating gene 2 locus. B Using a primer set for the interferon- V gene locus. M: markers. (Data kindly provided by S. zur Lage and S. Weiss, National Research Center for Biotechnology, Braunschweig, Germany.) Microsatellite Analysis with Optimized QIAGEN Protocol

    Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluoresceinlabeled primers. Reactions were analyzed on the ABI PRISM 377 Sequencer. Top: High sensitivity and uniform signal intensity using the QIAGEN Multiplex PCR Kit. Bottom: Results using a hot-start DNA polymerase from Supplier AII.



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    Plasmid expressing PLAP reporter gene....
    Puromycin resistance gene (pac), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
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