( which can be incorporated into any ex...)QIAGEN Multiplex PCR Kit

, which can be incorporated into any existing thermal-cycler program.

Successful 16plex PCR Using the QIAGEN Multiplex PCR Kit
Multiplex PCR of 16 targets (99-955 bp)
was carried out for 35 cycles using standard
conditions for the QIAGEN Multiplex PCR Kit
without further optimization or using a variety
of conditions with a hot-start DNA polymerase
from Supplier AII. A Comparison using 2.5 units
per 50 lreaction of the hot-start DNA polymerase
from Supplier AII and with the indicated Mg2+
concentrations. B Comparison using the optimized
Mg2+ concentration (3.5 mM) for the hot-start
DNA polymerase from Supplier AII (part A) and
the indicated amounts of enzyme per 50 l reaction.
M: markers. Procedure

The QIAGEN Multiplex PCR Kit contains a ready-to-use pre-optimized master mix, to minimize pipetting steps and eliminate tedious calculations. Only primers and template need to be added to prepare the final amplification mix. The master mix can be stored at 2-8C allowing even faster setup of multiplex PCR assays.

Applications

The QIAGEN Multiplex PCR Kit is highly suited for many types of multiplex applications, including:

• Typing and analysis of transgenic organisms (see figure: "Genotyping Transgenic Mice")
  
• Amplification and analysis of microsatellites (see figure: "Microsatellite Analysis with Optimized QI
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