( ...)QIAGEN Multiplex PCR Kit

The QIAGEN Multiplex PCR Kit is designed for highly specific and sensitive multiplex PCR without the need for time-consuming optimization

Features and benefits

• No optimization required easy assay development with convenient ready-to-use master mix
  
• High specificity and sensitivity stringent hot start with HotStarTaq DNA Polymerase and increased sensitivity with the unique new multiplex PCR buffer
  
• Versatile - highly suited for many types of multiplex PCR applications
  
• Easy-to-use and cost-effective simple reaction setup for fast and reproducible results

Principle

The QIAGEN Multiplex PCR Kit is the first kit specifically developed for multiplex PCR and provides an easy-to-use master-mix format. QIAGEN Multiplex PCR Master Mix contains pre-optimized concentrations of HotStarTaq DNA Polymerase and MgCl2, plus dNTPs and an innovative PCR buffer specially developed for multiplex PCR. The new PCR buffer contains a balanced combination of salts and additives, which enables comparable efficiencies for annealing and extension of all primers in the reaction (see figure: "Successful 16plex PCR Using the QIAGEN Multiplex PCR Kit"). Nonspecific annealing is minimized, which leads to maximal yields of specific PCR products. The stringent hot start provided by HotStarTaq DNA Polymerase increases multiplex reaction specificity by preventing extension of nonspecifically annealed primers and primer-dimers. Multiplex assays can be set up at room temperature and HotStarTaq DNA Polymerase in the master mix is then activated by a 15-minute incubation at 95C
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