Navigation Links
QIAGEN Multiplex PCR Kit

The QIAGEN Multiplex PCR Kit is designed for highly specific and sensitive multiplex PCR without the need for time-consuming optimization

Features and benefits
  • No optimization required easy assay development with convenient ready-to-use master mix
  • High specificity and sensitivity stringent hot start with HotStarTaq DNA Polymerase and increased sensitivity with the unique new multiplex PCR buffer
  • Versatile - highly suited for many types of multiplex PCR applications
  • Easy-to-use and cost-effective simple reaction setup for fast and reproducible results
Principle

The QIAGEN Multiplex PCR Kit is the first kit specifically developed for multiplex PCR and provides an easy-to-use master-mix format. QIAGEN Multiplex PCR Master Mix contains pre-optimized concentrations of HotStarTaq DNA Polymerase and MgCl2, plus dNTPs and an innovative PCR buffer specially developed for multiplex PCR. The new PCR buffer contains a balanced combination of salts and additives, which enables comparable efficiencies for annealing and extension of all primers in the reaction (see figure: "Successful 16plex PCR Using the QIAGEN Multiplex PCR Kit"). Nonspecific annealing is minimized, which leads to maximal yields of specific PCR products. The stringent hot start provided by HotStarTaq DNA Polymerase increases multiplex reaction specificity by preventing extension of nonspecifically annealed primers and primer-dimers. Multiplex assays can be set up at room temperature and HotStarTaq DNA Polymerase in the master mix is then activated by a 15-minute incubation at 95C, which can be incorporated into any existing thermal-cycler program.

Successful 16plex PCR Using the QIAGEN Multiplex PCR Kit
Multiplex PCR of 16 targets (99-955 bp)
was carried out for 35 cycles using standard
conditions for the QIAGEN Multiplex PCR Kit
without further optimization or using a variety
of conditions with a hot-start DNA polymerase
from Supplier AII. A Comparison using 2.5 units
per 50 lreaction of the hot-start DNA polymerase
from Supplier AII and with the indicated Mg2+
concentrations. B Comparison using the optimized
Mg2+ concentration (3.5 mM) for the hot-start
DNA polymerase from Supplier AII (part A) and
the indicated amounts of enzyme per 50 l reaction.
M: markers. Procedure

The QIAGEN Multiplex PCR Kit contains a ready-to-use pre-optimized master mix, to minimize pipetting steps and eliminate tedious calculations. Only primers and template need to be added to prepare the final amplification mix. The master mix can be stored at 2-8C allowing even faster setup of multiplex PCR assays.

Applications

The QIAGEN Multiplex PCR Kit is highly suited for many types of multiplex applications, including:

Genotyping Transgenic Mice

Transgenic mice were screened using the QIAGEN Multiplex PCR Kit and sets of 3 primers to distinguish wild-type (wt), heterozygous mutant (ht), and homozygous mutant (hm) mice. A Using a primer set for the recombination activating gene 2 locus. B Using a primer set for the interferon- V gene locus. M: markers. (Data kindly provided by S. zur Lage and S. Weiss, National Research Center for Biotechnology, Braunschweig, Germany.) Microsatellite Analysis with Optimized QIAGEN Protocol

Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluoresceinlabeled primers. Reactions were analyzed on the ABI PRISM 377 Sequencer. Top: High sensitivity and uniform signal intensity using the QIAGEN Multiplex PCR Kit. Bottom: Results using a hot-start DNA polymerase from Supplier AII.



'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. The QIAGEN Guide to Animal Cell Culture
2. The QIAGEN Guide to Animal Cell Culture
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. QIAGEN Instrument Service insist on the best in service and support
8. QIAGEN Multiplex PCR Handbook
9. QIAGEN Plasmid Kits
10. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
11. QIAGEN PCR CloningPlus Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: QIAGEN Multiplex PCR Kit

(Date:6/1/2015)... , June 1, 2015 ... ) has announced the addition of the ... (Consumables, Instruments, Services), Test Type (Indirect, Enzyme-based, ... Flow Cytometry), End Users - Global Forecast ...      (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ...
(Date:6/1/2015)... 2015 /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" or "the Company") today ... Biorem,s complete 2015 first quarter financial statements and MD&A have been ... March 31,(in CDN$,000 except per share data) , ... 1,974 Gross profit , 1,531 , 329 ... , 528 , (531) Basic earnings (loss) per ...
(Date:6/1/2015)... , May 29, 2015 Research ... addition of the "Global Bioinformatics Market 2015-2019" ... analysts forecast the global bioinformatics market to grow ... 2014-2019. Bioinformatics is a field of science ... consists of information stored in DNA form and ...
(Date:6/1/2015)... Frederick MD (PRWEB) June 01, 2015 ... that they have entered into a commercial collaboration ... both companies will combine their technologies and work ... cell (MSC) regenerative technology. RoosterBio and Sistemic will ... and technologies to build more precise and relevant ...
Breaking Biology Technology:Oxidative Stress Assay Market 2015 - Global Forecast to 2020 2Biorem Reports Increased Revenues and Earnings for First Quarter 2Biorem Reports Increased Revenues and Earnings for First Quarter 3Global Bioinformatics Market 2015-2019 - Rapid Developments in Molecular Diagnostics 2Global Bioinformatics Market 2015-2019 - Rapid Developments in Molecular Diagnostics 3Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 2Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 3Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 4
... Biotechnology, Inc. (OTC: CSBR) ("Champions") announced today the ... Inc. in which Champions will conduct low passage ... an inhibitor of mutant B-Raf, and CEP-37440, a ... determine the activity or response in potential clinical ...
... Laboratory, Inc. (HDL, Inc.) and BG Medicine, Inc. (Nasdaq: ... Inc. will offer galectin-3 testing services based on the BG ... Test was recently cleared by the FDA as an aid ... Elevated galectin-3 levels are associated with an inherently progressive form ...
... The Ontario Institute for Cancer Research (OICR) ... accelerate commercialization of a potentially revolutionary high-throughput screening ... The technology, Smart Well Plate™, developed at the ... utilizes digital microfluidics (DMF), a technique that allows ...
Cached Biology Technology:Champions Announces Technology Collaboration Utilizing Champions' Tumorgraft™ Technology Platform for Oncology Drug Development 2Champions Announces Technology Collaboration Utilizing Champions' Tumorgraft™ Technology Platform for Oncology Drug Development 3Health Diagnostic Laboratory, Inc. (HDL, Inc.) Announces Availability of Galectin-3 Testing Service in Partnership With BG Medicine 2Health Diagnostic Laboratory, Inc. (HDL, Inc.) Announces Availability of Galectin-3 Testing Service in Partnership With BG Medicine 3Health Diagnostic Laboratory, Inc. (HDL, Inc.) Announces Availability of Galectin-3 Testing Service in Partnership With BG Medicine 4Ontario Institute for Cancer Research Announces Investment in Microfluidics Technology for the Discovery of Anti-Cancer Drugs 2Ontario Institute for Cancer Research Announces Investment in Microfluidics Technology for the Discovery of Anti-Cancer Drugs 3
(Date:4/17/2015)... 17, 2015 Increasing ... technological advancement to drive biometric systems market in ...   According to a recently released ... &  Opportunities, 2020 ", biometric systems market in ... growth at CAGR of over 22% through 2020. ...
(Date:4/14/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... growing mobile commerce market, announces that its Wocket® smart ... Design Awards under the category  ,Product Design – Technology,. ... are part of a global multi-disciplinary awards program. The ... marketplace, industry and judging panel. Winners will be announced ...
(Date:4/13/2015)... CHICAGO , April 13, 2015  higi, a ... with ways to more fully engage with their communities ... its industry-leading, privacy protected and secure API.  ... API enables the most accessible, affordable, and convenient vehicle ... The API will ...
Breaking Biology News(10 mins):Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3higi Launches Seamless Access to HIPAA-Compliant Health Outcomes and Activity Data 2higi Launches Seamless Access to HIPAA-Compliant Health Outcomes and Activity Data 3
... expressions of different types are detected in a crowd ... faces are a priority for visual processing ?particularly for ... of the Massachusetts Institute of Technology and Jason Mattingley ... the June 6th issue of Current Biology. , ...
... demonstrated that learning about the appearance of their parents ... parental traits, rather than traits that more exactly match ... help drive the evolution of exaggerated traits and strong ... and other animals. The findings are reported by Carel ...
... of Michigan Kellogg Eye Center have gained new insight into ... itself to a specific role. They have observed a small ... to play a particular role, but has not yet begun ... vantage point for understanding how a healthy visual system develops. ...
Cached Biology News:Zebra finch males prefer females with exaggerated maternal traits 2Study provides first look at the 'birth' of a retina cell 2Study provides first look at the 'birth' of a retina cell 3
Tracker Datamatrix TRxD reads any 1D and 2D barcodes within 0.5 seconds....
Complete cell culture media with cytokines...
... BioChain's monkey normal multiple tissue ... specific cellular localization of genes or ... tissues. Tissues are mounted on positively ... Blots are available in the identical ...
8 different frozen tissues are mounted on positively charged glass slides...
Biology Products: