( ...)QIAGEN Multiplex PCR Handbook

For fast and efficient multiplex PCR


Contents
Kit Contents
Shipping Conditions
Storage and Stability
Technical Assistance
Safety Information
Product Use Limitations
Product Warranty and Satisfaction Guarantee
Introduction
HotStarTaq DNA Polymerase
QIAGEN Multiplex PCR Buffer
Q-Solution
Product Description
Quality Control
Solutions and Reagents to Be Supplied by User
Protocols for Multiplex PCR Standard Multiplex PCR Protocol
Multiplex PCR Protocol for Amplification of Microsatellite Loci
Multiplex PCR Protocol Using Q-Solution Troubleshooting Guide
Appendix 1. Starting template
2. General guidelines for multiplex PCR primer design
3. Handling and storage of primers
4. Number of PCR cycles
5. Separation of multiplex PCR products using agarose gel elect
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