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QIAGEN LongRange PCR and 2Step RT-PCR Kits

use kit contains an enhanced viral reverse transcriptase, providing highly sensitive synthesis of full-length cDNA (up to 12.5 kb) even from low-abundance targets (Figure 2). The LongRange RT Buffer is specially formulated to provide efficient reverse transcription with no optimization required. The novel RNase H activity quencher binds to the cDNA:RNA hybridbinding site of the reverse transcriptase preventing degradation of the RNA during synthesis and enabling generation of full-length cDNA (Figure 3). The LongRange 2Step RT-PCR Kit also contains all of the components necesssary for subsequent long-range PCR.





Amplification of rare and difficult templates
The unique zwitterionic formulation of LongRange PCR Buffer improves pH buffering at high temperatures (7294C), minimizing pH-driven template degradation. In combination with Q-Solution (provided), it further facilitates amplification of GC-rich and other difficult templates.


Applications
The QIAGEN LongRange PCR and 2Step RT-PCR Kits provide accurate long-range amplification of standard and complex templates (even low-abundance or GC-rich templates). Both kits are ideal for all PCR applications including cloning, sequencing, and gene expression.







Find out more about our long-range PCR products at www.qiagen.com/goto/PCR


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