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QIAGEN LongRange PCR and 2Step RT-PCR Kits

For reliable and accurate long-range PCR and RT-PCR


QIAGEN LongRange PCR and LongRange 2Step RT-PCR Kits provide dedicated and highly reliable solutions for long-range amplification from genomic DNA and cDNA. Low error rates are assured using a novel high-fidelity enzyme mix.


QIAGEN LongRange PCR and 2Step RT-PCR Kits provide:

Amplification of extremely long PCR products up to 40 kb DNA or 12.5 kb cDNA

Low error rates ensured by high-fidelity enzyme mix

Minimal PCR optimization due to unique buffer system

Amplification of difficult templates including low-copy targets and GC-rich templates


A dedicated solution for long-range DNA amplification
The QIAGEN LongRange PCR Kit contains all of the components required for the amplification of long PCR fragments. A unique blend of thermostable DNA polymerases, with enhanced processivity, allows the amplification of full-length PCR products. The enzyme mix provides high extension rates and proofreading ability for increased fidelity (up to 5-fold higher fidelity than Taq DNA polymerase). The novel buffer system provides highly specific long-range PCR, even from complex genomic DNA (Figure 1). The kit also contains nucleotides and Q-Solution an additive for the amplification of GC-rich templates. Preoptimized protocols make the QIAGEN LongRange PCR Kit easy and convenient to use.


A dedicated solution for long-range two-step RT-PCR
The QIAGEN LongRange 2Step RT-PCR Kit combines the features and benefits of the QIAGEN LongRange PCR Kit with a high-yield, full-length reverse transcription step. This complete and ready-to- use kit contains an enhanced viral reverse transcriptase, providing highly sensitive synthesis of full-length cDNA (up to 12.5 kb) even from low-abundance targets (Figure 2). The LongRange RT Buffer is specially formulated to provide efficient reverse transcription with no optimization required. The novel RNase H activity quencher binds to the cDNA:RNA hybridbinding site of the reverse transcriptase preventing degradation of the RNA during synthesis and enabling generation of full-length cDNA (Figure 3). The LongRange 2Step RT-PCR Kit also contains all of the components necesssary for subsequent long-range PCR.





Amplification of rare and difficult templates
The unique zwitterionic formulation of LongRange PCR Buffer improves pH buffering at high temperatures (7294C), minimizing pH-driven template degradation. In combination with Q-Solution (provided), it further facilitates amplification of GC-rich and other difficult templates.


Applications
The QIAGEN LongRange PCR and 2Step RT-PCR Kits provide accurate long-range amplification of standard and complex templates (even low-abundance or GC-rich templates). Both kits are ideal for all PCR applications including cloning, sequencing, and gene expression.







Find out more about our long-range PCR products at www.qiagen.com/goto/PCR


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