Navigation Links
QIAGEN LongRange PCR and 2Step RT-PCR Kits

For reliable and accurate long-range PCR and RT-PCR


QIAGEN LongRange PCR and LongRange 2Step RT-PCR Kits provide dedicated and highly reliable solutions for long-range amplification from genomic DNA and cDNA. Low error rates are assured using a novel high-fidelity enzyme mix.


QIAGEN LongRange PCR and 2Step RT-PCR Kits provide:

Amplification of extremely long PCR products up to 40 kb DNA or 12.5 kb cDNA

Low error rates ensured by high-fidelity enzyme mix

Minimal PCR optimization due to unique buffer system

Amplification of difficult templates including low-copy targets and GC-rich templates


A dedicated solution for long-range DNA amplification
The QIAGEN LongRange PCR Kit contains all of the components required for the amplification of long PCR fragments. A unique blend of thermostable DNA polymerases, with enhanced processivity, allows the amplification of full-length PCR products. The enzyme mix provides high extension rates and proofreading ability for increased fidelity (up to 5-fold higher fidelity than Taq DNA polymerase). The novel buffer system provides highly specific long-range PCR, even from complex genomic DNA (Figure 1). The kit also contains nucleotides and Q-Solution an additive for the amplification of GC-rich templates. Preoptimized protocols make the QIAGEN LongRange PCR Kit easy and convenient to use.


A dedicated solution for long-range two-step RT-PCR
The QIAGEN LongRange 2Step RT-PCR Kit combines the features and benefits of the QIAGEN LongRange PCR Kit with a high-yield, full-length reverse transcription step. This complete and ready-to- use kit contains an enhanced viral reverse transcriptase, providing highly sensitive synthesis of full-length cDNA (up to 12.5 kb) even from low-abundance targets (Figure 2). The LongRange RT Buffer is specially formulated to provide efficient reverse transcription with no optimization required. The novel RNase H activity quencher binds to the cDNA:RNA hybridbinding site of the reverse transcriptase preventing degradation of the RNA during synthesis and enabling generation of full-length cDNA (Figure 3). The LongRange 2Step RT-PCR Kit also contains all of the components necesssary for subsequent long-range PCR.





Amplification of rare and difficult templates
The unique zwitterionic formulation of LongRange PCR Buffer improves pH buffering at high temperatures (7294C), minimizing pH-driven template degradation. In combination with Q-Solution (provided), it further facilitates amplification of GC-rich and other difficult templates.


Applications
The QIAGEN LongRange PCR and 2Step RT-PCR Kits provide accurate long-range amplification of standard and complex templates (even low-abundance or GC-rich templates). Both kits are ideal for all PCR applications including cloning, sequencing, and gene expression.







Find out more about our long-range PCR products at www.qiagen.com/goto/PCR


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. The QIAGEN Guide to Animal Cell Culture
2. The QIAGEN Guide to Animal Cell Culture
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. QIAGEN Instrument Service insist on the best in service and support
8. QIAGEN Multiplex PCR Handbook
9. QIAGEN Multiplex PCR Kit
10. QIAGEN Plasmid Kits
11. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/12/2017)... 12, 2017 The report "Direct-Fed Microbials Market by Type (Lactic Acid ... Liquid), and Region - Global Forecast to 2022", published by MarketsandMarkets, the global ... projected to reach USD 1,399.6 Million by 2022, at a CAGR of 6.96% ... ... MarketsandMarkets Logo ...
(Date:1/11/2017)... ... January 11, 2017 , ... ... specialists with accurate and reliable preimplantation genetic screening (PGS). , “Our genetics and ... results,” says Ovation Fertility Genetics Scientific Director Amy Jones, M.S., ELD (ABB) ...
(Date:1/11/2017)... Jan. 11, 2017  Brian Mehling, M.D., world-renowned stem ... Blue Horizon International (BHI), will be attending the 47th ... from January 17-20, 2017. This will be ... The theme of this year,s forum is Responsive ... sessions will address strategies for fostering greater social inclusion ...
(Date:1/11/2017)... Francisco, CA (PRWEB) , ... January 11, 2017 ... ... series of in-kind scientific grants to ground-breaking microbiome studies. Its most recent microbiome ... School of Medicine, who will study the effect of long-term use of oral ...
Breaking Biology Technology:
(Date:12/22/2016)... Calif. , Dec. 20, 2016  As part of ... 23andMe, the leading personal genetics company, recently released its latest ... . The book focuses on the topics of inheritance ... Generation Science Standards (NGSS) taught in elementary school classrooms in ... in a series by illustrator Ariana Killoran , whose ...
(Date:12/19/2016)... TORONTO , 19 de diciembre de 2016  Mosaic Biomedicals ... el desarrollo acelerado de MSC-1, un anticuerpo humanizado que se espera ... en 2017, con múltiples sitios previstos a lo largo de Europa ... MSC-1 ... factor inhibidor de leucemia (LIF), una citoquina pleiotrópica que se sobreexpresa ...
(Date:12/15/2016)... Germany , December 15, 2016 ... today announced an agreement with NuData Security, an award-winning ... The partnership will enable clients to focus on good customer ... data protection regulation. ... In order to provide a one-stop fraud prevention ...
Breaking Biology News(10 mins):