Navigation Links
QIAGEN LongRange PCR and 2Step RT-PCR Kits

For reliable and accurate long-range PCR and RT-PCR

QIAGEN LongRange PCR and LongRange 2Step RT-PCR Kits provide dedicated and highly reliable solutions for long-range amplification from genomic DNA and cDNA. Low error rates are assured using a novel high-fidelity enzyme mix.

QIAGEN LongRange PCR and 2Step RT-PCR Kits provide:

Amplification of extremely long PCR products up to 40 kb DNA or 12.5 kb cDNA

Low error rates ensured by high-fidelity enzyme mix

Minimal PCR optimization due to unique buffer system

Amplification of difficult templates including low-copy targets and GC-rich templates

A dedicated solution for long-range DNA amplification
The QIAGEN LongRange PCR Kit contains all of the components required for the amplification of long PCR fragments. A unique blend of thermostable DNA polymerases, with enhanced processivity, allows the amplification of full-length PCR products. The enzyme mix provides high extension rates and proofreading ability for increased fidelity (up to 5-fold higher fidelity than Taq DNA polymerase). The novel buffer system provides highly specific long-range PCR, even from complex genomic DNA (Figure 1). The kit also contains nucleotides and Q-Solution an additive for the amplification of GC-rich templates. Preoptimized protocols make the QIAGEN LongRange PCR Kit easy and convenient to use.

A dedicated solution for long-range two-step RT-PCR
The QIAGEN LongRange 2Step RT-PCR Kit combines the features and benefits of the QIAGEN LongRange PCR Kit with a high-yield, full-length reverse transcription step. This complete and ready-to- use kit contains an enhanced viral reverse transcriptase, providing highly sensitive synthesis of full-length cDNA (up to 12.5 kb) even from low-abundance targets (Figure 2). The LongRange RT Buffer is specially formulated to provide efficient reverse transcription with no optimization required. The novel RNase H activity quencher binds to the cDNA:RNA hybridbinding site of the reverse transcriptase preventing degradation of the RNA during synthesis and enabling generation of full-length cDNA (Figure 3). The LongRange 2Step RT-PCR Kit also contains all of the components necesssary for subsequent long-range PCR.

Amplification of rare and difficult templates
The unique zwitterionic formulation of LongRange PCR Buffer improves pH buffering at high temperatures (7294C), minimizing pH-driven template degradation. In combination with Q-Solution (provided), it further facilitates amplification of GC-rich and other difficult templates.

The QIAGEN LongRange PCR and 2Step RT-PCR Kits provide accurate long-range amplification of standard and complex templates (even low-abundance or GC-rich templates). Both kits are ideal for all PCR applications including cloning, sequencing, and gene expression.

Find out more about our long-range PCR products at

back to top


Page: All 1 2

Related biology technology :

1. The QIAGEN Guide to Animal Cell Culture
2. The QIAGEN Guide to Animal Cell Culture
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. QIAGEN Instrument Service insist on the best in service and support
8. QIAGEN Multiplex PCR Handbook
9. QIAGEN Multiplex PCR Kit
10. QIAGEN Plasmid Kits
11. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
Post Your Comments:

(Date:11/30/2015)... , Nov. 30, 2015  AbbVie, is introducing ... focuses on a daily routine for managing the life-long ... medication can affect the way the body absorbs it ... their a daily routine are important. The goal of ... patients better manage their hypothyroidism by establishing a daily ...
(Date:11/30/2015)... ... November 30, 2015 , ... ... integration with MarkLogic, the Enterprise NoSQL database platform provider, creating a seamless ... , Smartlogic’s Content Intelligence capabilities provide a robust set of semantic tools ...
(Date:11/27/2015)... , Nov. 27, 2015 /PRNewswire/--  Mallinckrodt plc (NYSE: ... today that it has closed the sale of its ... Guerbet (GBT- NYSE Euronext) in a transaction valued at ... manufacturing facilities and a total of approximately 1,000 employees ... St. Louis area. This entire ...
(Date:11/26/2015)... 26, 2015 ... Accutest Research Laboratories, a leading independent ... (CRO), has formed a strategic partnership ... Temple Health for joint work on ... ) , --> ...
Breaking Biology Technology:
(Date:11/10/2015)... YORK , Nov. 10, 2015 ... to behavioral biometrics that helps to identify and ... fraud. Signature is considered as the secure and ... the identification of a particular individual because each ... more accurate results especially when dynamic signature of ...
(Date:10/29/2015)...   MedNet Solutions , an innovative SaaS-based eClinical ... research, is pleased to announce that it has been ... one of only three finalists for a 2015 ... Growing" category. The Tekne Awards honor Minnesota ... innovation and leadership. iMedNet™ eClinical ...
(Date:10/29/2015)... Connecticut , October 29, 2015 ... a biometric authentication company focused on the growing ... smart wallet announces that StackCommerce, a leading marketplace ... be featuring the Wocket® smart wallet on StackSocial ... NXTD ) ("NXT-ID" or the "Company"), a biometric ...
Breaking Biology News(10 mins):