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QIAGEN Fast Cycling PCR Kit

Cycling PCR Buffer has been developed to eliminate the need for optimization of individual primertemplate systems, saving time and money. Specific amplification is maintained over a wide range of temperatures and Mg2+ concentrations, without the need for timeconsuming optimization


CoralLoad Fast Cycling Dye
QIAGEN Fast Cycling PCR Kit is supplied with new CoralLoad Fast Cycling Dye that enables direct loading of the reaction onto an agarose gel without the need to add a gel-loading buffer (Figure 5). CoralLoad Fast Cycling Dye contains two marker dyes an orange dye and a red dye that facilitate estimation of DNA migration distance and optimization of agarose gel run time.



Fast cycling PCR for everyone
The QIAGEN Fast Cycling PCR Kit enables increased PCR throughput using existing lab equipment. It can be used with any thermal cycler, including fast ramping cyclers, without loss of specificity (Figure 2). In addition, unlike other PCR chemistries, there is no need to modify or change your existing primers or optimized annealing temperatures. Each kit component has been specifically designed to offer convenient and reliable fast cycling PCR (Figure 6 and Table 2).




Q-Solution for amplification of difficult templates

QIAGEN Fast Cycling PCR Kit is also supplied with Q-Solution, an innovative PCR additive that facilitates amplification of difficult templates by modifying the melting behavior of DNA. This unique reagent will often enable or improve a suboptimal PCR caused by templates that have a high degree of secondary structure or that
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