( fast and specific amplification (see T...)QIAGEN Fast Cycling PCR Kit

High specificity and sensitivity
The QIAGEN Fast Cycling PCR Kit contains HotStarTaq Plus DNA Polymerase, which offers unrivalled hot-start PCR performance. HotStarTaq Plus DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. This prevents the formation of misprimed products and primerdimers at low temperatures (Figure 3) and also enables convenient room-temperature reaction setup.

Unlike other fast-cycling chemistries which often result in amplification failure, HotStarTaq Plus DNA Polymerase, in combination with the new QIAGEN Fast Cycling PCR Buffer promotes specific primertemplate annealing and simultaneously reduces nonspecific annealing (Figure 3). Amplification of nonspecific products, primer-dimer formation, and background smear are minimized in every PCR cycle. Q-Solution, a novel additive that enables efficient amplification of difficult (e.g., GC-rich) templates, is also provided.

Unique Fast Cycling PCR Buffer
The patent-pending Fast Cycling PCR Buffer contains the novel Q-Bond Molecule, which dramatically increases the binding affinity of DNA polymerase to single-stranded DNA, thereby facilitating the reduction of annealing time to just 5 seconds (Figure 4). The unique buffer formulation and optimized DNA polymerase concentration also enables a significant reduction in the denaturation and extension times.

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