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QIAGEN Fast Cycling PCR Kit

For fast and specific PCR on any thermal cycler


The QIAGEN Fast Cycling PCR Kit provides the unrivaled performance of HotStarTaq Plus DNA Polymerase with a significantly shortened PCR cycling time on any thermal cycler.


The QIAGEN Fast Cycling PCR Kit provides:

Ultrafast hot-start PCR time savings of up to 75%

Compatible with any thermal cycler ideal for all labs

High specificity and sensitivity successful results with minimal optimization

Easy and convenient handling optional ready-to-load PCR buffer and no primer redesign required





Ultrafast hot-start PCR
The innovative QIAGEN Fast Cycling PCR Kit provides highly specific and rapid amplification of PCR products using any thermal cycler. The significant time saving of up to 75% is achieved using a novel, patentpending PCR buffer formulation that significantly reduces the time required to form the polymerase, primer, and template complex. This allows the polymerase to extend the primers during the PCR annealing step thereby dramatically reducing the duration of annealing steps in every PCR cycle. PCR cycling times can be reduced from over 1 hour (for 35 cycles) to less than 20 minutes (Figure 1 and Table 1). Fast-ramping cyclers can achieve a total PCR time of just 30 minutes (Figure 2). These time savings make the QIAGEN Fast Cycling PCR Kit ideal for increasing PCR throughput without buying additional and expensive lab equipment.




The kit is provided in a master mix format, which includes all of the components necessary for
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