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For fast and specific PCR on any thermal cycler
The QIAGEN Fast Cycling PCR Kit provides the unrivaled performance of
HotStarTaq Plus DNA Polymerase with a significantly shortened PCR
cycling time on any thermal cycler.
The QIAGEN Fast Cycling PCR Kit provides:
Ultrafast hot-start PCR time savings of up to 75%
Compatible with any thermal cycler ideal for all labs
High specificity and sensitivity successful results with minimal optimization
Easy and convenient handling optional ready-to-load PCR buffer and no primer redesign required
Ultrafast hot-start PCR
The innovative QIAGEN Fast Cycling PCR Kit provides highly specific and
rapid amplification of PCR products using any thermal cycler. The significant
time saving of up to 75% is achieved using a novel, patentpending PCR
buffer formulation that significantly reduces the time required to form
the polymerase, primer, and template complex. This allows the polymerase
to extend the primers during the PCR annealing step thereby dramatically
reducing the duration of annealing steps in every PCR cycle. PCR cycling
times can be reduced from over 1 hour (for 35 cycles) to less than 20
minutes (Figure 1 and Table 1). Fast-ramping cyclers can achieve a total
PCR time of just 30 minutes (Figure 2). These time savings make the QIAGEN
Fast Cycling PCR Kit ideal for increasing PCR throughput without buying
additional and expensive lab equipment.
The kit is provided in a master mix format, which includes all of the
components necessary for
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