( promise the integrity of fragile adipoc...)QBT Fatty Acid Uptake Assay Kit datasheet

OPTIMIZED ASSAY
The QBT Fatty Acid Uptake Assay is a single-step homogeneous fluorescent assay that provides real-time uptake kinetics and is ideally suited for high throughput screening applications in both 96-well and 384-well formats. The kit employs a BODIPY-dodecanoic acid fluorescent fatty acid analog coupled with Molecular Devices proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids: it becomes activated by acyl-CoA attachment; is incorporated into di-and triglycerides; and accumulates in intracellular lipid droplets. In addition, the BODIPY analog is a known substrate for fatty acid transporters since its uptake by adipocytes can be competed by non-labeled fatty acids. The elimination of radioactive compounds results in easier reagent handling, reduced disposal costs and eliminates safety risks associated with radiolabel assays.

PATENTED TECHNOLOGY
QBT Assay Kits from Molecular Devices employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of QBT Assay Kits.

OBSERVE TRUE BIOLOGICAL ACTIVITY
Consistent with the observation that FATPs are only expressed by differentiated cells, 3T3 L1 adipocytes take up the BODIPY analog and accumulate it in lipid droplets. In contrast, undifferentiated 3T3L1 fibroblasts do not transport or store the dye. (See Figure 1.) Known activators and inhibitors of fatty acid uptake can be seen to stimulate or inhibit uptake in adipocytes in a dose-dependent manner, but show no effect on uptake in undifferentiated fibroblasts. (See Figures 2 and 3.)

RAPID AS
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