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β-Glucuronidase from E. coli K12 New improved quality!

 Roche Applied Science recently introduced an improved β-Glucuronidase (GRD) formulation which replaces the previous product. The new quality exhibits higher purity and specific activity compared with products previously on the market (Figure).



GRD hydrolyses β-glucuronic acid (β-GlcA) esters (β-glucuronosides) of a variety of compounds, including steroids, aryl alcohols, drugs, and metabolites. GRD also hydrolyses polysaccharides that contain β-GlcA linked to other sugars. GRD is highly specific for the carbohydrate portion of the molecule but has very little specificity for the molecule conjugated to β-GlcA. The main research applications are:
  • Analysis of steroid conjugates
  • Doping analysis (horses)
  • Drug and alcohol testing
  • Tests in pathology labs

The performance in steroid analysis and doping analysis was demonstrated by Ute Mareck, German Sport University, Cologne, Germany, and Thierry Rousseau, National Doping Testing Laboratory (LNDD), Chtenay- Malabry, France.




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