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Pwo Master*:,,,Simplify Setup of Your Proofreading PCRs

matic properties of the enzyme, resulting in higher yields and additional robustness of the amplification reaction without changing the fidelity of DNA synthesis.

Advantages of Pwo Master



Avoid the need to determine the optimal buffer conditions for your proofreading PCRs
  • The Mg2+ concentration of the master mix in combination with the specially formulated buffer system allows the amplification of most fragments up to 3 kb with high yield and specificity (Figure 1), and the high fidelity you expect from a proofreading polymerase (Figure 2).
  • There is no need to titrate the optimal ionic conditions for a given assay or to perform cumbersome adjustments of the reagent compositions (Figure 1).

Improve the reproducibility and consistency of your proofreading PCRs
  • The number of pipetting steps during PCR setup is reduced.
  • The risk of contaminating the reaction mix during PCR setup is reduced.
  • The analysis of your results is straightforward.

Save time the Pwo Master is ready when you are
  • The Pwo Master is stored at 2 8C and can be used immediately; no freezing or thawing is necessary.

Contents of Pwo Master
  • Pwo Master: 10 vials of double-concentrated master mix, 10 reactions per vial. Each vial contains 25 units PwoSUPER YIELD DNA Polymerase, reaction buffer with 4mM MgCl2 and PCR-Grade Nucleotides (dATP, dCTP, dGTP, dTTP, each 0.4 mM) in a total volume of 250 l.
  • PCR-Grade Water: four vi
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Related biology technology :

1. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
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