With the introduction of the Pwo Master, Roche Applied
Science now offers a new, ready-to-use proofreading
master mix for the robust amplification of fragments up to
3 kb, providing the highest possible fidelity during PCR
Pwo Master is a premixed double-concentrated formulation
that contains PwoSUPER YIELD DNA Polymerase,
a new optimized buffer system, and PCR-Grade
Nucleotides. After simply adding the template DNA and
the primers and adjusting the final volume to 50 l with
PCR-Grade Water (provided with the Pwo Master), the
reaction can be started.
The reaction setup of a large number of samples for PCR
analysis requires a multitude of pipetting and handling
steps. Every step in PCR is critical to the quality of the final
result; pipetting and handling errors, as well as assay contamination,
can negatively impact the experimental outcome,
leading to false-negative or false-positive results.
As a result, PCRs must often be repeated, resulting in
wasted effort and reagent expense. The availability of master mixes simplifies the PCR setup
and circumvents many problems.
Enzymology of Pwo Master
The core component of the Pwo Master is the proofreading
polymerase Pwo DNA Polymerase. Pwo DNA
Polymerase is a highly processive 5′3′ DNA polymerase
that possesses 3′5′ exonuclease activity, also known as proofreading activity. This proofreading activity
results in an approximate 18-fold increase in the
fidelity of DNA synthesis compared with Taq DNA polymerase.
PwoSUPER YIELD DNA Polymerase combines Pwo DNA
Polymerase with a new optimized buffer system which
enhances the enzy
Source:Page: All 1 2 3 Related biology technology :1
. Efficient and Reliable PCR Setup Using Eppendorf MasterMix2
. Comparing Fidelity and Performance of Proofreading PCR Enzymes