With the introduction of the Pwo Master, Roche Applied Science now offers a new, ready-to-use proofreading master mix for the robust amplification of fragments up to 3 kb, providing the highest possible fidelity during PCR synthesis.

Pwo Master is a premixed double-concentrated formulation that contains PwoSUPER YIELD DNA Polymerase, a new optimized buffer system, and PCR-Grade Nucleotides. After simply adding the template DNA and the primers and adjusting the final volume to 50 l with PCR-Grade Water (provided with the Pwo Master), the reaction can be started.

The reaction setup of a large number of samples for PCR analysis requires a multitude of pipetting and handling steps. Every step in PCR is critical to the quality of the final result; pipetting and handling errors, as well as assay contamination, can negatively impact the experimental outcome, leading to false-negative or false-positive results. As a result, PCRs must often be repeated, resulting in wasted effort and reagent expense. The availability of master mixes simplifies the PCR setup and circumvents many problems.

Enzymology of Pwo Master

The core component of the Pwo Master is the proofreading polymerase Pwo DNA Polymerase. Pwo DNA Polymerase is a highly processive 5′3′ DNA polymerase that possesses 3′5′ exonuclease activity, also known as proofreading activity. This proofreading activity results in an approximate 18-fold increase in the fidelity of DNA synthesis compared with Taq DNA polymerase.

PwoSUPER YIELD DNA Polymerase combines Pwo DNA Polymerase with a new optimized buffer system which enhances the enzy
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